DETERMINATION OF MICS & GENE MUTATIONS AMONG FOSFOMYCIN RESISTANT ISOLATES OF ASYMPTOMATIC BACTERIURIA CASES DURING PREGNANCY
Intro: Asymptomatic bacteriuria is a common finding during pregnancy. Effective antibiotic treatment could reduce its adverse effects during pregnancy. However, antimicrobial resistance limits the treatment options. Fosfomycin might be a promising drug in this regard as its resistance is still low....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-05-01
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| Series: | International Journal of Infectious Diseases |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S120197122300262X |
| _version_ | 1797824608823410688 |
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| author | A. Sultan S. Khatoon F. Khan T. Khan A. Singh |
| author_facet | A. Sultan S. Khatoon F. Khan T. Khan A. Singh |
| author_sort | A. Sultan |
| collection | DOAJ |
| description | Intro: Asymptomatic bacteriuria is a common finding during pregnancy. Effective antibiotic treatment could reduce its adverse effects during pregnancy. However, antimicrobial resistance limits the treatment options. Fosfomycin might be a promising drug in this regard as its resistance is still low. The aim of the study was to assess the MICs of fosfomycin resistant isolates & to investigate the genetic mutations responsible for fosfomycin resistance Methods: This was a 2-year study. Around 10252 urine samples from asymptomatic pregnant females (18-45 years) attending the Antinatal clinics were included in the study. Identification of pathogen & antimicrobial susceptibility testing was done as per standard methods. Fosfomycin resistance was confirmed by agar dilution. Presence of murA, fosA, uhpT & glpT genes were checked by Conventional PCR. Findings: Around 1173 (11.4%) pregnant females were found to have ASB. E. coli 495(42%) being the predominant organism. CRE were 296(40%), MRSA and VRE accounted for 50(66.6%) and 5(2%) respectively. Among oral antibiotics Cotrimoxazole showed (43%), Amoxiclav (6%), Cefixime (37%), Norfloxacin (38%), Nitrofurantoin (60%) susceptibilities while fosfomycin was found sensitive in almost 98% isolates. Only 22 isolates were found resistant to fosfomycin by disc diffusion method. On agar dilution one isolate was found sensitive showing MIC <64μg/ml. Of 21 isolates, fosfomycin MICs were; 256μg/ml (3 E.coli strains), 1024μg/ml (2 E.coli isolates) & rest 16 isolates (5 E.coli, 4 Klebsiella pneumonia, 2 Proteus spp, 2 E.faecalis, 3 Staphylococcus spp) showed MICs >2048μg/ml. On PCR, murA & glpT genes were present in 5& 4GN isolates respectively, while only 1GP isolate was positive for fosA gene. None of the strains showed the amplification of uhpT genes. Conclusion: mur A was commonest genes depicting chromosomal resistance. Fos A was found in only one Enterococcus faecalis indicating transferrable resistance. There was little discrepancy between disk diffusion and agar dilution methods. |
| first_indexed | 2024-03-13T10:41:31Z |
| format | Article |
| id | doaj.art-e9d51f875a524d8189fea9c1235401fa |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-03-13T10:41:31Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-e9d51f875a524d8189fea9c1235401fa2023-05-18T04:38:23ZengElsevierInternational Journal of Infectious Diseases1201-97122023-05-01130S53DETERMINATION OF MICS & GENE MUTATIONS AMONG FOSFOMYCIN RESISTANT ISOLATES OF ASYMPTOMATIC BACTERIURIA CASES DURING PREGNANCYA. Sultan0S. Khatoon1F. Khan2T. Khan3A. Singh4Aligarh Muslim University, Microbiology, Aligarh, IndiaAligarh Muslim University, Microbiology, Aligarh, IndiaAligarh Muslim University, Microbiology, Aligarh, IndiaAligarh Muslim University, Microbiology, Aligarh, IndiaAligarh Muslim University, Microbiology, Aligarh, IndiaIntro: Asymptomatic bacteriuria is a common finding during pregnancy. Effective antibiotic treatment could reduce its adverse effects during pregnancy. However, antimicrobial resistance limits the treatment options. Fosfomycin might be a promising drug in this regard as its resistance is still low. The aim of the study was to assess the MICs of fosfomycin resistant isolates & to investigate the genetic mutations responsible for fosfomycin resistance Methods: This was a 2-year study. Around 10252 urine samples from asymptomatic pregnant females (18-45 years) attending the Antinatal clinics were included in the study. Identification of pathogen & antimicrobial susceptibility testing was done as per standard methods. Fosfomycin resistance was confirmed by agar dilution. Presence of murA, fosA, uhpT & glpT genes were checked by Conventional PCR. Findings: Around 1173 (11.4%) pregnant females were found to have ASB. E. coli 495(42%) being the predominant organism. CRE were 296(40%), MRSA and VRE accounted for 50(66.6%) and 5(2%) respectively. Among oral antibiotics Cotrimoxazole showed (43%), Amoxiclav (6%), Cefixime (37%), Norfloxacin (38%), Nitrofurantoin (60%) susceptibilities while fosfomycin was found sensitive in almost 98% isolates. Only 22 isolates were found resistant to fosfomycin by disc diffusion method. On agar dilution one isolate was found sensitive showing MIC <64μg/ml. Of 21 isolates, fosfomycin MICs were; 256μg/ml (3 E.coli strains), 1024μg/ml (2 E.coli isolates) & rest 16 isolates (5 E.coli, 4 Klebsiella pneumonia, 2 Proteus spp, 2 E.faecalis, 3 Staphylococcus spp) showed MICs >2048μg/ml. On PCR, murA & glpT genes were present in 5& 4GN isolates respectively, while only 1GP isolate was positive for fosA gene. None of the strains showed the amplification of uhpT genes. Conclusion: mur A was commonest genes depicting chromosomal resistance. Fos A was found in only one Enterococcus faecalis indicating transferrable resistance. There was little discrepancy between disk diffusion and agar dilution methods.http://www.sciencedirect.com/science/article/pii/S120197122300262X |
| spellingShingle | A. Sultan S. Khatoon F. Khan T. Khan A. Singh DETERMINATION OF MICS & GENE MUTATIONS AMONG FOSFOMYCIN RESISTANT ISOLATES OF ASYMPTOMATIC BACTERIURIA CASES DURING PREGNANCY International Journal of Infectious Diseases |
| title | DETERMINATION OF MICS & GENE MUTATIONS AMONG FOSFOMYCIN RESISTANT ISOLATES OF ASYMPTOMATIC BACTERIURIA CASES DURING PREGNANCY |
| title_full | DETERMINATION OF MICS & GENE MUTATIONS AMONG FOSFOMYCIN RESISTANT ISOLATES OF ASYMPTOMATIC BACTERIURIA CASES DURING PREGNANCY |
| title_fullStr | DETERMINATION OF MICS & GENE MUTATIONS AMONG FOSFOMYCIN RESISTANT ISOLATES OF ASYMPTOMATIC BACTERIURIA CASES DURING PREGNANCY |
| title_full_unstemmed | DETERMINATION OF MICS & GENE MUTATIONS AMONG FOSFOMYCIN RESISTANT ISOLATES OF ASYMPTOMATIC BACTERIURIA CASES DURING PREGNANCY |
| title_short | DETERMINATION OF MICS & GENE MUTATIONS AMONG FOSFOMYCIN RESISTANT ISOLATES OF ASYMPTOMATIC BACTERIURIA CASES DURING PREGNANCY |
| title_sort | determination of mics amp gene mutations among fosfomycin resistant isolates of asymptomatic bacteriuria cases during pregnancy |
| url | http://www.sciencedirect.com/science/article/pii/S120197122300262X |
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