TEL/AML1 Fusion Gene in Childhood Acute Lymphoblastic Leukemia in Southern Taiwan
Chromosomal abnormalities are found in 80–90% of childhood cases of acute lymphoblastic leukemia (ALL). Leukemia-specific chromosome aberrations not only have prognostic value, but also provide important clues for further investigation into leukogenesis, leukemic cell transformation, and proliferati...
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| Format: | Article |
| Language: | English |
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Wiley
2008-06-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1607551X08701554 |
| _version_ | 1819104063701123072 |
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| author | Pei-Chin Lin Tai-Tsung Chang Shiu-Ru Lin Shyh-Shin Chiou Ren-Chin Jang Jiunn-Ming Sheen |
| author_facet | Pei-Chin Lin Tai-Tsung Chang Shiu-Ru Lin Shyh-Shin Chiou Ren-Chin Jang Jiunn-Ming Sheen |
| author_sort | Pei-Chin Lin |
| collection | DOAJ |
| description | Chromosomal abnormalities are found in 80–90% of childhood cases of acute lymphoblastic leukemia (ALL). Leukemia-specific chromosome aberrations not only have prognostic value, but also provide important clues for further investigation into leukogenesis, leukemic cell transformation, and proliferation. This study used reverse transcriptase–polymerase chain reaction techniques to detect transcripts of the leukemia-specific chromosome fusion gene, TEL/AML1, and to monitor the expression levels of the TEL-AML1 fusion transcript in ALL patients at sequential intervals during their treatment course. Twenty-five ALL patients were enrolled, including 20 who were newly diagnosed and five in relapse. The incidence of the TEL/AML1 fusion gene in this study was 32%. The clinical features of our eight TEL/AML1-positive ALL cases were similar to those in other studies. Blotting analysis of the levels of the TEL-AML1 fusion transcript was used to detect minimal residual disease. Reduced levels of TEL/AML1 expression were found in four of the six patients whose bone marrow or peripheral blood samples were obtained after treatment. Further investigation with a larger sample size is warranted. |
| first_indexed | 2024-12-22T02:00:24Z |
| format | Article |
| id | doaj.art-e9d881717fe940c6b9513b07421c51b4 |
| institution | Directory Open Access Journal |
| issn | 1607-551X |
| language | English |
| last_indexed | 2024-12-22T02:00:24Z |
| publishDate | 2008-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-e9d881717fe940c6b9513b07421c51b42022-12-21T18:42:39ZengWileyKaohsiung Journal of Medical Sciences1607-551X2008-06-0124628929610.1016/S1607-551X(08)70155-4TEL/AML1 Fusion Gene in Childhood Acute Lymphoblastic Leukemia in Southern TaiwanPei-Chin Lin0Tai-Tsung Chang1Shiu-Ru Lin2Shyh-Shin Chiou3Ren-Chin Jang4Jiunn-Ming Sheen5Division of Hematology/Oncology, Department of Pediatrics, Kaohsiung Medical University Hospital, TaiwanDivision of Hematology/Oncology, Department of Pediatrics, Kaohsiung Medical University Hospital, TaiwanGraduate Institute of Medical Genetics, Kaohsiung Medical University, Chang Gung Memorial Hospital, Kaohsiung, TaiwanDivision of Hematology/Oncology, Department of Pediatrics, Kaohsiung Medical University Hospital, TaiwanDivision of Hematology/Oncology, Department of Pediatrics, Kaohsiung Medical University Hospital, TaiwanDivision of Hematology/Oncology, Department of Pediatrics, Chang Gung Memorial Hospital, Kaohsiung, TaiwanChromosomal abnormalities are found in 80–90% of childhood cases of acute lymphoblastic leukemia (ALL). Leukemia-specific chromosome aberrations not only have prognostic value, but also provide important clues for further investigation into leukogenesis, leukemic cell transformation, and proliferation. This study used reverse transcriptase–polymerase chain reaction techniques to detect transcripts of the leukemia-specific chromosome fusion gene, TEL/AML1, and to monitor the expression levels of the TEL-AML1 fusion transcript in ALL patients at sequential intervals during their treatment course. Twenty-five ALL patients were enrolled, including 20 who were newly diagnosed and five in relapse. The incidence of the TEL/AML1 fusion gene in this study was 32%. The clinical features of our eight TEL/AML1-positive ALL cases were similar to those in other studies. Blotting analysis of the levels of the TEL-AML1 fusion transcript was used to detect minimal residual disease. Reduced levels of TEL/AML1 expression were found in four of the six patients whose bone marrow or peripheral blood samples were obtained after treatment. Further investigation with a larger sample size is warranted.http://www.sciencedirect.com/science/article/pii/S1607551X08701554acute lymphoblastic leukemiapolymerase chain reactionTEL/AML1 fusion gene |
| spellingShingle | Pei-Chin Lin Tai-Tsung Chang Shiu-Ru Lin Shyh-Shin Chiou Ren-Chin Jang Jiunn-Ming Sheen TEL/AML1 Fusion Gene in Childhood Acute Lymphoblastic Leukemia in Southern Taiwan Kaohsiung Journal of Medical Sciences acute lymphoblastic leukemia polymerase chain reaction TEL/AML1 fusion gene |
| title | TEL/AML1 Fusion Gene in Childhood Acute Lymphoblastic Leukemia in Southern Taiwan |
| title_full | TEL/AML1 Fusion Gene in Childhood Acute Lymphoblastic Leukemia in Southern Taiwan |
| title_fullStr | TEL/AML1 Fusion Gene in Childhood Acute Lymphoblastic Leukemia in Southern Taiwan |
| title_full_unstemmed | TEL/AML1 Fusion Gene in Childhood Acute Lymphoblastic Leukemia in Southern Taiwan |
| title_short | TEL/AML1 Fusion Gene in Childhood Acute Lymphoblastic Leukemia in Southern Taiwan |
| title_sort | tel aml1 fusion gene in childhood acute lymphoblastic leukemia in southern taiwan |
| topic | acute lymphoblastic leukemia polymerase chain reaction TEL/AML1 fusion gene |
| url | http://www.sciencedirect.com/science/article/pii/S1607551X08701554 |
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