Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods
Abstract This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-flotation using Sheather's solu...
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| Format: | Article |
| Language: | English |
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Colégio Brasileiro de Parasitologia Veterinaria
2018-03-01
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| Series: | Revista Brasileira de Parasitologia Veterinária |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018005002104&lng=en&tlng=en |
| _version_ | 1811245620701167616 |
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| author | Vinícius da Silva Camargo Bruna Nicoleti Santana Elis Domingos Ferrari Alex Akira Nakamura Walter Bertequini Nagata Ana Rita Moraes Nardi Marcelo Vasconcelos Meireles |
| author_facet | Vinícius da Silva Camargo Bruna Nicoleti Santana Elis Domingos Ferrari Alex Akira Nakamura Walter Bertequini Nagata Ana Rita Moraes Nardi Marcelo Vasconcelos Meireles |
| author_sort | Vinícius da Silva Camargo |
| collection | DOAJ |
| description | Abstract This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-flotation using Sheather's solution. Cryptosporidium spp. diagnosis was performed using three diagnostic methods: malachite green negative staining, nested PCR targeting the 18S rRNA gene, followed by sequencing the amplified fragments, and duplex real-time PCR targeting the 18S rRNA specific to detect Cryptosporidium galli and Cryptosporidium avian genotype III. The overall positivity for Cryptosporidium spp. (total samples positive in at least one protocol) from the microscopic analysis, nested PCR and duplex real-time PCR protocol results was 13.3% (66/498). The positivity rates were 2.0% (10/498) and 4.6% (23/498) for Cryptosporidium spp. by microscopy and nested PCR, respectively. Sequencing of 20 samples amplified by nested PCR identified C. galli (3.0%; 15/498), Cryptosporidium avian genotype I (0.8%; 4/498) and Cryptosporidium avium (0.2%; 1/498). Duplex real-time PCR revealed a positivity of 7.8% (39/498) for C. galli and 2.4% (12/498) for avian genotype III. Malachite green negative staining differed significantly from nested PCR in detecting Cryptosporidium spp. Duplex real-time PCR was more sensitive than nested PCR/sequencing for detecting gastric Cryptosporidium in canaries. |
| first_indexed | 2024-04-12T14:42:06Z |
| format | Article |
| id | doaj.art-e9d994ae770446bb8d18f8d925b020c6 |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-04-12T14:42:06Z |
| publishDate | 2018-03-01 |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-e9d994ae770446bb8d18f8d925b020c62022-12-22T03:28:48ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-29612018-03-01010.1590/s1984-296120180010S1984-29612018005002104Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methodsVinícius da Silva CamargoBruna Nicoleti SantanaElis Domingos FerrariAlex Akira NakamuraWalter Bertequini NagataAna Rita Moraes NardiMarcelo Vasconcelos MeirelesAbstract This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-flotation using Sheather's solution. Cryptosporidium spp. diagnosis was performed using three diagnostic methods: malachite green negative staining, nested PCR targeting the 18S rRNA gene, followed by sequencing the amplified fragments, and duplex real-time PCR targeting the 18S rRNA specific to detect Cryptosporidium galli and Cryptosporidium avian genotype III. The overall positivity for Cryptosporidium spp. (total samples positive in at least one protocol) from the microscopic analysis, nested PCR and duplex real-time PCR protocol results was 13.3% (66/498). The positivity rates were 2.0% (10/498) and 4.6% (23/498) for Cryptosporidium spp. by microscopy and nested PCR, respectively. Sequencing of 20 samples amplified by nested PCR identified C. galli (3.0%; 15/498), Cryptosporidium avian genotype I (0.8%; 4/498) and Cryptosporidium avium (0.2%; 1/498). Duplex real-time PCR revealed a positivity of 7.8% (39/498) for C. galli and 2.4% (12/498) for avian genotype III. Malachite green negative staining differed significantly from nested PCR in detecting Cryptosporidium spp. Duplex real-time PCR was more sensitive than nested PCR/sequencing for detecting gastric Cryptosporidium in canaries.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018005002104&lng=en&tlng=enCryptosporidiosisbirdsdiagnosisepidemiology |
| spellingShingle | Vinícius da Silva Camargo Bruna Nicoleti Santana Elis Domingos Ferrari Alex Akira Nakamura Walter Bertequini Nagata Ana Rita Moraes Nardi Marcelo Vasconcelos Meireles Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods Revista Brasileira de Parasitologia Veterinária Cryptosporidiosis birds diagnosis epidemiology |
| title | Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods |
| title_full | Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods |
| title_fullStr | Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods |
| title_full_unstemmed | Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods |
| title_short | Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods |
| title_sort | detection and molecular characterization of cryptosporidium spp in captive canaries serinus canaria using different diagnostic methods |
| topic | Cryptosporidiosis birds diagnosis epidemiology |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612018005002104&lng=en&tlng=en |
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