Targeted genome editing in polyploids: lessons from Brassica
CRISPR-mediated genome editing has emerged as a powerful tool for creating targeted mutations in the genome for various applications, including studying gene functions, engineering resilience against biotic and abiotic stresses, and increasing yield and quality. However, its utilization is limited t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1152468/full |
| _version_ | 1827921356201656320 |
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| author | Niaz Ahmad Samia Fatima Muhammad Aamer Mehmood Qamar U. Zaman Qamar U. Zaman Rana Muhammad Atif Rana Muhammad Atif Weijun Zhou Mehboob-ur Rahman Rafaqat Ali Gill |
| author_facet | Niaz Ahmad Samia Fatima Muhammad Aamer Mehmood Qamar U. Zaman Qamar U. Zaman Rana Muhammad Atif Rana Muhammad Atif Weijun Zhou Mehboob-ur Rahman Rafaqat Ali Gill |
| author_sort | Niaz Ahmad |
| collection | DOAJ |
| description | CRISPR-mediated genome editing has emerged as a powerful tool for creating targeted mutations in the genome for various applications, including studying gene functions, engineering resilience against biotic and abiotic stresses, and increasing yield and quality. However, its utilization is limited to model crops for which well-annotated genome sequences are available. Many crops of dietary and economic importance, such as wheat, cotton, rapeseed-mustard, and potato, are polyploids with complex genomes. Therefore, progress in these crops has been hampered due to genome complexity. Excellent work has been conducted on some species of Brassica for its improvement through genome editing. Although excellent work has been conducted on some species of Brassica for genome improvement through editing, work on polyploid crops, including U’s triangle species, holds numerous implications for improving other polyploid crops. In this review, we summarize key examples from genome editing work done on Brassica and discuss important considerations for deploying CRISPR-mediated genome editing more efficiently in other polyploid crops for improvement. |
| first_indexed | 2024-03-13T04:25:21Z |
| format | Article |
| id | doaj.art-e9daa9b287ef4debbc54c2484893f64b |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-13T04:25:21Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-e9daa9b287ef4debbc54c2484893f64b2023-06-20T06:45:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-06-011410.3389/fpls.2023.11524681152468Targeted genome editing in polyploids: lessons from BrassicaNiaz Ahmad0Samia Fatima1Muhammad Aamer Mehmood2Qamar U. Zaman3Qamar U. Zaman4Rana Muhammad Atif5Rana Muhammad Atif6Weijun Zhou7Mehboob-ur Rahman8Rafaqat Ali Gill9National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Faisalabad, PakistanNational Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Faisalabad, PakistanDepartment of Bioinformatics & Biotechnology, Government College University Faisalabad, Faisalabad, PakistanHainan Yazhou Bay Seed Laboratory, Sanya Nanfan Research Institute of Hainan University, Sanya, ChinaCollege of Tropical Crops, Hainan University, Haikou, ChinaNational Center of Genome Editing, Center of Advanced Studies, Agriculture and Food Security, University of Agriculture, Faisalabad, PakistanDepartment of Plant Breeding and Genetics, University of Agriculture Faisalabad, Faisalabad, PakistanMinistry of Agriculture and Rural Affairs Key Lab of Spectroscopy Sensing, Institute of Crop Science, Zhejiang University, Hangzhou, ChinaNational Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Faisalabad, PakistanKey Laboratory for Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, ChinaCRISPR-mediated genome editing has emerged as a powerful tool for creating targeted mutations in the genome for various applications, including studying gene functions, engineering resilience against biotic and abiotic stresses, and increasing yield and quality. However, its utilization is limited to model crops for which well-annotated genome sequences are available. Many crops of dietary and economic importance, such as wheat, cotton, rapeseed-mustard, and potato, are polyploids with complex genomes. Therefore, progress in these crops has been hampered due to genome complexity. Excellent work has been conducted on some species of Brassica for its improvement through genome editing. Although excellent work has been conducted on some species of Brassica for genome improvement through editing, work on polyploid crops, including U’s triangle species, holds numerous implications for improving other polyploid crops. In this review, we summarize key examples from genome editing work done on Brassica and discuss important considerations for deploying CRISPR-mediated genome editing more efficiently in other polyploid crops for improvement.https://www.frontiersin.org/articles/10.3389/fpls.2023.1152468/fullgenome editingCRISPRBrassicapolyploid cropscrop improvement |
| spellingShingle | Niaz Ahmad Samia Fatima Muhammad Aamer Mehmood Qamar U. Zaman Qamar U. Zaman Rana Muhammad Atif Rana Muhammad Atif Weijun Zhou Mehboob-ur Rahman Rafaqat Ali Gill Targeted genome editing in polyploids: lessons from Brassica Frontiers in Plant Science genome editing CRISPR Brassica polyploid crops crop improvement |
| title | Targeted genome editing in polyploids: lessons from Brassica |
| title_full | Targeted genome editing in polyploids: lessons from Brassica |
| title_fullStr | Targeted genome editing in polyploids: lessons from Brassica |
| title_full_unstemmed | Targeted genome editing in polyploids: lessons from Brassica |
| title_short | Targeted genome editing in polyploids: lessons from Brassica |
| title_sort | targeted genome editing in polyploids lessons from brassica |
| topic | genome editing CRISPR Brassica polyploid crops crop improvement |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1152468/full |
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