Applicability and Safety of in Vitro Skin Expansion Using a Skin Bioreactor: A Clinical Trial
Background Tissue expansion is an effective and valuable technique for the reconstruction of large skin lesions and scars. This study aimed to evaluate the applicability and safety of a newly designed skin expanding bioreactor system for maximizing the graft area and minimizing the donor site area....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Thieme Medical Publishers, Inc.
2014-11-01
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| Series: | Archives of Plastic Surgery |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.5999/aps.2014.41.6.661 |
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| author | Cheol Jeong Ho Yun Chung Hyun Ju Lim Jeong Woo Lee Kang Young Choi Jung Dug Yang Byung Chae Cho Jeong Ok Lim James J Yoo Sang Jin Lee Anthony J Atala |
| author_facet | Cheol Jeong Ho Yun Chung Hyun Ju Lim Jeong Woo Lee Kang Young Choi Jung Dug Yang Byung Chae Cho Jeong Ok Lim James J Yoo Sang Jin Lee Anthony J Atala |
| author_sort | Cheol Jeong |
| collection | DOAJ |
| description | Background Tissue expansion is an effective and valuable technique for the reconstruction of large skin lesions and scars. This study aimed to evaluate the applicability and safety of a newly designed skin expanding bioreactor system for maximizing the graft area and minimizing the donor site area.
Methods A computer-controlled biaxial skin bioreactor system was used to expand skin in two directions while the culture media was changed daily. The aim was to achieve an expansion speed that enabled the skin to reach twice its original area in two weeks or less. Skin expansion and subsequent grafting were performed for 10 patients, and each patient was followed for 6 months postoperatively for clinical evaluation. Scar evaluation was performed through visual assessment and by using photos.
Results The average skin expansion rate was 10.54%±6.25%; take rate, 88.89%±11.39%; and contraction rate, 4.2%±2.28% after 6 months. Evaluation of the donor and recipient sites by medical specialists resulted in an average score of 3.5 (out of a potential maximum of 5) at 3 months, and 3.9 at 6 months. The average score for patient satisfaction of the donor site was 6.2 (out of a potential maximum of 10), and an average score of 5.2 was noted for the recipient site. Histological examination performed before and after the skin expansion revealed an increase in porosity of the dermal layer.
Conclusions This study confirmed the safety and applicability of the in vitro skin bioreactor, and further studies are needed to develop methods for increasing the skin expansion rate. |
| first_indexed | 2024-12-10T06:21:31Z |
| format | Article |
| id | doaj.art-e9db17ad8ab240c7b0b8872df90a0a1a |
| institution | Directory Open Access Journal |
| issn | 2234-6163 2234-6171 |
| language | English |
| last_indexed | 2024-12-10T06:21:31Z |
| publishDate | 2014-11-01 |
| publisher | Thieme Medical Publishers, Inc. |
| record_format | Article |
| series | Archives of Plastic Surgery |
| spelling | doaj.art-e9db17ad8ab240c7b0b8872df90a0a1a2022-12-22T01:59:20ZengThieme Medical Publishers, Inc.Archives of Plastic Surgery2234-61632234-61712014-11-01410666166710.5999/aps.2014.41.6.661426Applicability and Safety of in Vitro Skin Expansion Using a Skin Bioreactor: A Clinical TrialCheol Jeong0Ho Yun Chung1Hyun Ju Lim2Jeong Woo Lee3Kang Young Choi4Jung Dug Yang5Byung Chae Cho6Jeong Ok Lim7James J Yoo8Sang Jin Lee9Anthony J Atala10Department of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, KoreaDepartment of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, KoreaWake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USADepartment of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, KoreaDepartment of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, KoreaDepartment of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, KoreaDepartment of Plastic and Reconstructive Surgery, Kyungpook National University School of Medicine, Daegu, KoreaBiomedical Research Institute, Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Kyungpook National University School of Medicine, Daegu, KoreaWake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USAWake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USAWake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USABackground Tissue expansion is an effective and valuable technique for the reconstruction of large skin lesions and scars. This study aimed to evaluate the applicability and safety of a newly designed skin expanding bioreactor system for maximizing the graft area and minimizing the donor site area. Methods A computer-controlled biaxial skin bioreactor system was used to expand skin in two directions while the culture media was changed daily. The aim was to achieve an expansion speed that enabled the skin to reach twice its original area in two weeks or less. Skin expansion and subsequent grafting were performed for 10 patients, and each patient was followed for 6 months postoperatively for clinical evaluation. Scar evaluation was performed through visual assessment and by using photos. Results The average skin expansion rate was 10.54%±6.25%; take rate, 88.89%±11.39%; and contraction rate, 4.2%±2.28% after 6 months. Evaluation of the donor and recipient sites by medical specialists resulted in an average score of 3.5 (out of a potential maximum of 5) at 3 months, and 3.9 at 6 months. The average score for patient satisfaction of the donor site was 6.2 (out of a potential maximum of 10), and an average score of 5.2 was noted for the recipient site. Histological examination performed before and after the skin expansion revealed an increase in porosity of the dermal layer. Conclusions This study confirmed the safety and applicability of the in vitro skin bioreactor, and further studies are needed to develop methods for increasing the skin expansion rate.http://www.thieme-connect.de/DOI/DOI?10.5999/aps.2014.41.6.661skin transplantationtissue expander devicescicatrix |
| spellingShingle | Cheol Jeong Ho Yun Chung Hyun Ju Lim Jeong Woo Lee Kang Young Choi Jung Dug Yang Byung Chae Cho Jeong Ok Lim James J Yoo Sang Jin Lee Anthony J Atala Applicability and Safety of in Vitro Skin Expansion Using a Skin Bioreactor: A Clinical Trial Archives of Plastic Surgery skin transplantation tissue expander devices cicatrix |
| title | Applicability and Safety of in Vitro Skin Expansion Using a Skin Bioreactor: A Clinical Trial |
| title_full | Applicability and Safety of in Vitro Skin Expansion Using a Skin Bioreactor: A Clinical Trial |
| title_fullStr | Applicability and Safety of in Vitro Skin Expansion Using a Skin Bioreactor: A Clinical Trial |
| title_full_unstemmed | Applicability and Safety of in Vitro Skin Expansion Using a Skin Bioreactor: A Clinical Trial |
| title_short | Applicability and Safety of in Vitro Skin Expansion Using a Skin Bioreactor: A Clinical Trial |
| title_sort | applicability and safety of in vitro skin expansion using a skin bioreactor a clinical trial |
| topic | skin transplantation tissue expander devices cicatrix |
| url | http://www.thieme-connect.de/DOI/DOI?10.5999/aps.2014.41.6.661 |
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