Differential proteomics and physiology of <it>Pseudomonas putida</it> KT2440 under filament-inducing conditions
<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas putida</it> exerts a filamentous phenotype in response to environmental stress conditions that are encountered during its natural life cycle. This study assessed whether <it>P. putida</it> fila...
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| Format: | Article |
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BMC
2012-11-01
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| Series: | BMC Microbiology |
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| Online Access: | http://www.biomedcentral.com/1471-2180/12/282 |
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| author | Crabbé Aurélie Leroy Baptiste Wattiez Ruddy Aertsen Abram Leys Natalie Cornelis Pierre Van Houdt Rob |
| author_facet | Crabbé Aurélie Leroy Baptiste Wattiez Ruddy Aertsen Abram Leys Natalie Cornelis Pierre Van Houdt Rob |
| author_sort | Crabbé Aurélie |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Pseudomonas putida</it> exerts a filamentous phenotype in response to environmental stress conditions that are encountered during its natural life cycle. This study assessed whether <it>P. putida</it> filamentation could confer survival advantages. Filamentation of <it>P. putida</it> was induced through culturing at low shaking speed and was compared to culturing in high shaking speed conditions, after which whole proteomic analysis and stress exposure assays were performed.</p> <p>Results</p> <p><it>P. putida</it> grown in filament-inducing conditions showed increased resistance to heat and saline stressors compared to non-filamented cultures. Proteomic analysis showed a significant metabolic change and a pronounced induction of the heat shock protein IbpA and recombinase RecA in filament-inducing conditions. Our data further indicated that the associated heat shock resistance, but not filamentation, was dependent of RecA.</p> <p>Conclusions</p> <p>This study provides insights into the altered metabolism of <it>P. putida</it> in filament-inducing conditions, and indicates that the formation of filaments could potentially be utilized by <it>P. putida</it> as a survival strategy in its hostile, recurrently changing habitat.</p> |
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| format | Article |
| id | doaj.art-e9dbbfdd19d8495093300c76cd0409c9 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-10T10:45:48Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
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| spelling | doaj.art-e9dbbfdd19d8495093300c76cd0409c92022-12-22T01:52:11ZengBMCBMC Microbiology1471-21802012-11-0112128210.1186/1471-2180-12-282Differential proteomics and physiology of <it>Pseudomonas putida</it> KT2440 under filament-inducing conditionsCrabbé AurélieLeroy BaptisteWattiez RuddyAertsen AbramLeys NatalieCornelis PierreVan Houdt Rob<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas putida</it> exerts a filamentous phenotype in response to environmental stress conditions that are encountered during its natural life cycle. This study assessed whether <it>P. putida</it> filamentation could confer survival advantages. Filamentation of <it>P. putida</it> was induced through culturing at low shaking speed and was compared to culturing in high shaking speed conditions, after which whole proteomic analysis and stress exposure assays were performed.</p> <p>Results</p> <p><it>P. putida</it> grown in filament-inducing conditions showed increased resistance to heat and saline stressors compared to non-filamented cultures. Proteomic analysis showed a significant metabolic change and a pronounced induction of the heat shock protein IbpA and recombinase RecA in filament-inducing conditions. Our data further indicated that the associated heat shock resistance, but not filamentation, was dependent of RecA.</p> <p>Conclusions</p> <p>This study provides insights into the altered metabolism of <it>P. putida</it> in filament-inducing conditions, and indicates that the formation of filaments could potentially be utilized by <it>P. putida</it> as a survival strategy in its hostile, recurrently changing habitat.</p>http://www.biomedcentral.com/1471-2180/12/282<it>Pseudomonas putida</it> KT2440FilamentationElongationSOS responseRecAShaking speedStress resistance |
| spellingShingle | Crabbé Aurélie Leroy Baptiste Wattiez Ruddy Aertsen Abram Leys Natalie Cornelis Pierre Van Houdt Rob Differential proteomics and physiology of <it>Pseudomonas putida</it> KT2440 under filament-inducing conditions BMC Microbiology <it>Pseudomonas putida</it> KT2440 Filamentation Elongation SOS response RecA Shaking speed Stress resistance |
| title | Differential proteomics and physiology of <it>Pseudomonas putida</it> KT2440 under filament-inducing conditions |
| title_full | Differential proteomics and physiology of <it>Pseudomonas putida</it> KT2440 under filament-inducing conditions |
| title_fullStr | Differential proteomics and physiology of <it>Pseudomonas putida</it> KT2440 under filament-inducing conditions |
| title_full_unstemmed | Differential proteomics and physiology of <it>Pseudomonas putida</it> KT2440 under filament-inducing conditions |
| title_short | Differential proteomics and physiology of <it>Pseudomonas putida</it> KT2440 under filament-inducing conditions |
| title_sort | differential proteomics and physiology of it pseudomonas putida it kt2440 under filament inducing conditions |
| topic | <it>Pseudomonas putida</it> KT2440 Filamentation Elongation SOS response RecA Shaking speed Stress resistance |
| url | http://www.biomedcentral.com/1471-2180/12/282 |
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