PCR Duplication: A One-Step Cloning-Free Method to Generate Duplicated Chromosomal Loci and Interference-Free Expression Reporters in Yeast.

PCR Duplication: A One-Step Cloning-Free Method to Generate Duplicated Chromosomal Loci and Interference-Free Expression Reporters in Yeast.

Here, we report on a novel PCR targeting-based strategy called 'PCR duplication' that enables targeted duplications of genomic regions in the yeast genome using a simple PCR-based approach. To demonstrate its application we first duplicated the promoter of the FAR1 gene in yeast and simult...

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Bibliographic Details
Main Authors: Florian Huber, Matthias Meurer, Daria Bunina, Ilia Kats, Céline I Maeder, Martin Stefl, Cyril Mongis, Michael Knop
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4262419?pdf=render

Internet

http://europepmc.org/articles/PMC4262419?pdf=render

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