Imipramine Induces Brain-Derived Neurotrophic Factor mRNA Expression in Cultured Astrocytes
Depression is one of the most prevalent and livelihood-threatening forms of mental illnesses and the neural circuitry underlying depression remains incompletely understood. Recent studies suggest that the neuronal plasticity involved with brain-derived neurotrophic factor (BDNF) plays an important r...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2012-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319304323 |
| _version_ | 1811288217840779264 |
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| author | Katsura Takano Hiroshi Yamasaki Kenji Kawabe Mitsuaki Moriyama Yoichi Nakamura |
| author_facet | Katsura Takano Hiroshi Yamasaki Kenji Kawabe Mitsuaki Moriyama Yoichi Nakamura |
| author_sort | Katsura Takano |
| collection | DOAJ |
| description | Depression is one of the most prevalent and livelihood-threatening forms of mental illnesses and the neural circuitry underlying depression remains incompletely understood. Recent studies suggest that the neuronal plasticity involved with brain-derived neurotrophic factor (BDNF) plays an important role in the recovery from depression. Some antidepressants are reported to induce BDNF expression in vivo; however, the mechanisms have been considered solely in neurons and not fully elucidated. In the present study, we evaluated the effects of imipramine, a classic tricyclic antidepressant drug, on BDNF expression in cultured rat brain astrocytes. Imipramine dose-dependently increased BDNF mRNA expression in astrocytes. The imipramine-induced BDNF increase was suppressed with inhibitors for protein kinase A (PKA) or MEK/ERK. Moreover, imipramine exposure activated transcription factor cAMP response element binding protein (CREB) in a dose-dependent manner. These results suggested that imipramine induced BDNF expression through CREB activation via PKA and/or ERK pathways. Imipramine treatment in depression might exert antidepressant action through BDNF production from astrocytes, and glial BDNF expression might be a target of developing novel antidepressants. Keywords:: astrocyte, antidepressant drug, brain-derived neurotrophic factor (BDNF), protein kinase A (PKA), cAMP response element binding protein (CREB) |
| first_indexed | 2024-04-13T03:33:14Z |
| format | Article |
| id | doaj.art-e9dc1a8257c946c7a6fc6c962824691d |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-04-13T03:33:14Z |
| publishDate | 2012-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-e9dc1a8257c946c7a6fc6c962824691d2022-12-22T03:04:25ZengElsevierJournal of Pharmacological Sciences1347-86132012-01-011203176186Imipramine Induces Brain-Derived Neurotrophic Factor mRNA Expression in Cultured AstrocytesKatsura Takano0Hiroshi Yamasaki1Kenji Kawabe2Mitsuaki Moriyama3Yoichi Nakamura4Laboratory of Integrative Physiology in Veterinary Sciences, Osaka Prefecture University, 1-58, Rinku-Ourai Kita, Izumisano, Osaka 598-8531, Japan; Corresponding author. takano@vet.osakafu-u.ac.jpLaboratory of Integrative Physiology in Veterinary Sciences, Osaka Prefecture University, 1-58, Rinku-Ourai Kita, Izumisano, Osaka 598-8531, JapanLaboratory of Integrative Physiology in Veterinary Sciences, Osaka Prefecture University, 1-58, Rinku-Ourai Kita, Izumisano, Osaka 598-8531, JapanLaboratory of Integrative Physiology in Veterinary Sciences, Osaka Prefecture University, 1-58, Rinku-Ourai Kita, Izumisano, Osaka 598-8531, JapanLaboratory of Integrative Physiology in Veterinary Sciences, Osaka Prefecture University, 1-58, Rinku-Ourai Kita, Izumisano, Osaka 598-8531, JapanDepression is one of the most prevalent and livelihood-threatening forms of mental illnesses and the neural circuitry underlying depression remains incompletely understood. Recent studies suggest that the neuronal plasticity involved with brain-derived neurotrophic factor (BDNF) plays an important role in the recovery from depression. Some antidepressants are reported to induce BDNF expression in vivo; however, the mechanisms have been considered solely in neurons and not fully elucidated. In the present study, we evaluated the effects of imipramine, a classic tricyclic antidepressant drug, on BDNF expression in cultured rat brain astrocytes. Imipramine dose-dependently increased BDNF mRNA expression in astrocytes. The imipramine-induced BDNF increase was suppressed with inhibitors for protein kinase A (PKA) or MEK/ERK. Moreover, imipramine exposure activated transcription factor cAMP response element binding protein (CREB) in a dose-dependent manner. These results suggested that imipramine induced BDNF expression through CREB activation via PKA and/or ERK pathways. Imipramine treatment in depression might exert antidepressant action through BDNF production from astrocytes, and glial BDNF expression might be a target of developing novel antidepressants. Keywords:: astrocyte, antidepressant drug, brain-derived neurotrophic factor (BDNF), protein kinase A (PKA), cAMP response element binding protein (CREB)http://www.sciencedirect.com/science/article/pii/S1347861319304323 |
| spellingShingle | Katsura Takano Hiroshi Yamasaki Kenji Kawabe Mitsuaki Moriyama Yoichi Nakamura Imipramine Induces Brain-Derived Neurotrophic Factor mRNA Expression in Cultured Astrocytes Journal of Pharmacological Sciences |
| title | Imipramine Induces Brain-Derived Neurotrophic Factor mRNA Expression in Cultured Astrocytes |
| title_full | Imipramine Induces Brain-Derived Neurotrophic Factor mRNA Expression in Cultured Astrocytes |
| title_fullStr | Imipramine Induces Brain-Derived Neurotrophic Factor mRNA Expression in Cultured Astrocytes |
| title_full_unstemmed | Imipramine Induces Brain-Derived Neurotrophic Factor mRNA Expression in Cultured Astrocytes |
| title_short | Imipramine Induces Brain-Derived Neurotrophic Factor mRNA Expression in Cultured Astrocytes |
| title_sort | imipramine induces brain derived neurotrophic factor mrna expression in cultured astrocytes |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319304323 |
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