Changes of BMP-2 and BMP-4 in early phase of sepsis
Objective To study the change of serum bone morphogenetic protein (BMP)-2 and BMP-4 during the development of sepsis and its mechanisms. Methods Blood was collected for quantifying serum BMP-2 and BMP-4 from patients with sepsis (sepsis group), patients without sepsis (non-sepsis group) in 24 hours...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-04-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-4-628.pdf |
| _version_ | 1797361466823671808 |
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| author | WEN Yan, LIN Jin-can, HUANG Ying-hong, XIAO Wen-biao, ZHANG Min-wei, LIN Jian-dong |
| author_facet | WEN Yan, LIN Jin-can, HUANG Ying-hong, XIAO Wen-biao, ZHANG Min-wei, LIN Jian-dong |
| author_sort | WEN Yan, LIN Jin-can, HUANG Ying-hong, XIAO Wen-biao, ZHANG Min-wei, LIN Jian-dong |
| collection | DOAJ |
| description | Objective To study the change of serum bone morphogenetic protein (BMP)-2 and BMP-4 during the development of sepsis and its mechanisms. Methods Blood was collected for quantifying serum BMP-2 and BMP-4 from patients with sepsis (sepsis group), patients without sepsis (non-sepsis group) in 24 hours of ICU admission and from healthy volunteers (control group). Concentration of serum BMP-2 and BMP-4 was determined by ELISA. The expression of BMP-2, pSmad1/5, and BMPRⅡ of lip polysaccharide (LPS)-treated rat pulmonary microvascular endothelial cells (RPMECs) was detected by Western blot. Results Serum BMP-2 and BMP-4 were significantly increased in sepsis group (P＜0.05) and the level of BMP-2 was significantly higher in sepsis group than that in non-sepsis group (P＜0.05). BMP-2 was found to be a marker for differential diagnosis of separating sepsis LPS may significantly increase the expression of BMP-2 of RPMECs but decrease the expression of phosphorylated Smad1/5 (pSmad1/5) and BMP receptor Ⅱ (BMPRⅡ) (P＜0.05). Conclusions BMP-2 is a potential diagnostic marker and act as apro-inflammatory factor in early phase of sepsis. |
| first_indexed | 2024-03-08T15:54:07Z |
| format | Article |
| id | doaj.art-e9dc4aa8445f4e46b877d7ddb9ea5ef4 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T15:54:07Z |
| publishDate | 2022-04-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-e9dc4aa8445f4e46b877d7ddb9ea5ef42024-01-09T02:51:38ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-04-0142462863210.16352/j.issn.1001-6325.2022.04.020Changes of BMP-2 and BMP-4 in early phase of sepsisWEN Yan, LIN Jin-can, HUANG Ying-hong, XIAO Wen-biao, ZHANG Min-wei, LIN Jian-dong01. Department of Intensive Care Unit, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005; ;2. Department of Intensive Care Unit, the First Affiliated Hospital of Xiamen University, Xiamen 361003, ChinaObjective To study the change of serum bone morphogenetic protein (BMP)-2 and BMP-4 during the development of sepsis and its mechanisms. Methods Blood was collected for quantifying serum BMP-2 and BMP-4 from patients with sepsis (sepsis group), patients without sepsis (non-sepsis group) in 24 hours of ICU admission and from healthy volunteers (control group). Concentration of serum BMP-2 and BMP-4 was determined by ELISA. The expression of BMP-2, pSmad1/5, and BMPRⅡ of lip polysaccharide (LPS)-treated rat pulmonary microvascular endothelial cells (RPMECs) was detected by Western blot. Results Serum BMP-2 and BMP-4 were significantly increased in sepsis group (P＜0.05) and the level of BMP-2 was significantly higher in sepsis group than that in non-sepsis group (P＜0.05). BMP-2 was found to be a marker for differential diagnosis of separating sepsis LPS may significantly increase the expression of BMP-2 of RPMECs but decrease the expression of phosphorylated Smad1/5 (pSmad1/5) and BMP receptor Ⅱ (BMPRⅡ) (P＜0.05). Conclusions BMP-2 is a potential diagnostic marker and act as apro-inflammatory factor in early phase of sepsis.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-4-628.pdfsepsis|bmp-2|bmp/psmad|pulmonary microvascular endothelial cells |
| spellingShingle | WEN Yan, LIN Jin-can, HUANG Ying-hong, XIAO Wen-biao, ZHANG Min-wei, LIN Jian-dong Changes of BMP-2 and BMP-4 in early phase of sepsis Jichu yixue yu linchuang sepsis|bmp-2|bmp/psmad|pulmonary microvascular endothelial cells |
| title | Changes of BMP-2 and BMP-4 in early phase of sepsis |
| title_full | Changes of BMP-2 and BMP-4 in early phase of sepsis |
| title_fullStr | Changes of BMP-2 and BMP-4 in early phase of sepsis |
| title_full_unstemmed | Changes of BMP-2 and BMP-4 in early phase of sepsis |
| title_short | Changes of BMP-2 and BMP-4 in early phase of sepsis |
| title_sort | changes of bmp 2 and bmp 4 in early phase of sepsis |
| topic | sepsis|bmp-2|bmp/psmad|pulmonary microvascular endothelial cells |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-4-628.pdf |
| work_keys_str_mv | AT wenyanlinjincanhuangyinghongxiaowenbiaozhangminweilinjiandong changesofbmp2andbmp4inearlyphaseofsepsis |