Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology
The cell membrane is a protective barrier whose configuration determines the exchange both between intracellular and extracellular regions and within the cell itself. Consequently, characterizing membrane properties and interactions is essential for advancements in topics such as limiting nanopartic...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Membranes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0375/11/5/319 |
| _version_ | 1797536081375133696 |
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| author | Joyce El-Beyrouthy Eric Freeman |
| author_facet | Joyce El-Beyrouthy Eric Freeman |
| author_sort | Joyce El-Beyrouthy |
| collection | DOAJ |
| description | The cell membrane is a protective barrier whose configuration determines the exchange both between intracellular and extracellular regions and within the cell itself. Consequently, characterizing membrane properties and interactions is essential for advancements in topics such as limiting nanoparticle cytotoxicity. Characterization is often accomplished by recreating model membranes that approximate the structure of cellular membranes in a controlled environment, formed using self-assembly principles. The selected method for membrane creation influences the properties of the membrane assembly, including their response to electric fields used for characterizing transmembrane exchanges. When these self-assembled model membranes are combined with electrophysiology, it is possible to exploit their non-physiological mechanics to enable additional measurements of membrane interactions and phenomena. This review describes several common model membranes including liposomes, pore-spanning membranes, solid supported membranes, and emulsion-based membranes, emphasizing their varying structure due to the selected mode of production. Next, electrophysiology techniques that exploit these structures are discussed, including conductance measurements, electrowetting and electrocompression analysis, and electroimpedance spectroscopy. The focus of this review is linking each membrane assembly technique to the properties of the resulting membrane, discussing how these properties enable alternative electrophysiological approaches to measuring membrane characteristics and interactions. |
| first_indexed | 2024-03-10T11:54:33Z |
| format | Article |
| id | doaj.art-e9e4e6e485c14724b18c698ca6d12ca2 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-10T11:54:33Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-e9e4e6e485c14724b18c698ca6d12ca22023-11-21T17:27:49ZengMDPI AGMembranes2077-03752021-04-0111531910.3390/membranes11050319Characterizing the Structure and Interactions of Model Lipid Membranes Using ElectrophysiologyJoyce El-Beyrouthy0Eric Freeman1School of Environmental, Civil, Agricultural and Mechanical Engineering, College of Engineering, University of Georgia, Athens, GA 30602, USASchool of Environmental, Civil, Agricultural and Mechanical Engineering, College of Engineering, University of Georgia, Athens, GA 30602, USAThe cell membrane is a protective barrier whose configuration determines the exchange both between intracellular and extracellular regions and within the cell itself. Consequently, characterizing membrane properties and interactions is essential for advancements in topics such as limiting nanoparticle cytotoxicity. Characterization is often accomplished by recreating model membranes that approximate the structure of cellular membranes in a controlled environment, formed using self-assembly principles. The selected method for membrane creation influences the properties of the membrane assembly, including their response to electric fields used for characterizing transmembrane exchanges. When these self-assembled model membranes are combined with electrophysiology, it is possible to exploit their non-physiological mechanics to enable additional measurements of membrane interactions and phenomena. This review describes several common model membranes including liposomes, pore-spanning membranes, solid supported membranes, and emulsion-based membranes, emphasizing their varying structure due to the selected mode of production. Next, electrophysiology techniques that exploit these structures are discussed, including conductance measurements, electrowetting and electrocompression analysis, and electroimpedance spectroscopy. The focus of this review is linking each membrane assembly technique to the properties of the resulting membrane, discussing how these properties enable alternative electrophysiological approaches to measuring membrane characteristics and interactions.https://www.mdpi.com/2077-0375/11/5/319model membraneselectrophysiologymembrane-particle interactionslipid bilayer electrostaticsconductive channelssoft capacitor |
| spellingShingle | Joyce El-Beyrouthy Eric Freeman Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology Membranes model membranes electrophysiology membrane-particle interactions lipid bilayer electrostatics conductive channels soft capacitor |
| title | Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology |
| title_full | Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology |
| title_fullStr | Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology |
| title_full_unstemmed | Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology |
| title_short | Characterizing the Structure and Interactions of Model Lipid Membranes Using Electrophysiology |
| title_sort | characterizing the structure and interactions of model lipid membranes using electrophysiology |
| topic | model membranes electrophysiology membrane-particle interactions lipid bilayer electrostatics conductive channels soft capacitor |
| url | https://www.mdpi.com/2077-0375/11/5/319 |
| work_keys_str_mv | AT joyceelbeyrouthy characterizingthestructureandinteractionsofmodellipidmembranesusingelectrophysiology AT ericfreeman characterizingthestructureandinteractionsofmodellipidmembranesusingelectrophysiology |