An Epitope Platform for Safe and Effective HTLV-1-Immunization: Potential Applications for mRNA and Peptide-Based Vaccines
Human T-cell lymphotropic virus type 1 (HTLV-1) infection affects millions of individuals worldwide and can lead to severe leukemia, myelopathy/tropical spastic paraparesis, and numerous other disorders. Pursuing a safe and effective immunotherapeutic approach, we compared the viral polyprotein and...
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MDPI AG
2021-07-01
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Online Access: | https://www.mdpi.com/1999-4915/13/8/1461 |
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author | Guglielmo Lucchese Hamid Reza Jahantigh Leonarda De Benedictis Piero Lovreglio Angela Stufano |
author_facet | Guglielmo Lucchese Hamid Reza Jahantigh Leonarda De Benedictis Piero Lovreglio Angela Stufano |
author_sort | Guglielmo Lucchese |
collection | DOAJ |
description | Human T-cell lymphotropic virus type 1 (HTLV-1) infection affects millions of individuals worldwide and can lead to severe leukemia, myelopathy/tropical spastic paraparesis, and numerous other disorders. Pursuing a safe and effective immunotherapeutic approach, we compared the viral polyprotein and the human proteome with a sliding window approach in order to identify oligopeptide sequences unique to the virus. The immunological relevance of the viral unique oligopeptides was assessed by searching them in the immune epitope database (IEDB). We found that HTLV-1 has 15 peptide stretches each consisting of uniquely viral non-human pentapeptides which are ideal candidate for a safe and effective anti-HTLV-1 vaccine. Indeed, experimentally validated HTLV-1 epitopes, as retrieved from the IEDB, contain peptide sequences also present in a vast number of human proteins, thus potentially instituting the basis for cross-reactions. We found a potential for cross-reactivity between the virus and the human proteome and described an epitope platform to be used in order to avoid it, thus obtaining effective, specific, and safe immunization. Potential advantages for mRNA and peptide-based vaccine formulations are discussed. |
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id | doaj.art-e9e582ef8ae14a3fb403e6287d2624f6 |
institution | Directory Open Access Journal |
issn | 1999-4915 |
language | English |
last_indexed | 2024-03-10T08:19:03Z |
publishDate | 2021-07-01 |
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series | Viruses |
spelling | doaj.art-e9e582ef8ae14a3fb403e6287d2624f62023-11-22T10:09:38ZengMDPI AGViruses1999-49152021-07-01138146110.3390/v13081461An Epitope Platform for Safe and Effective HTLV-1-Immunization: Potential Applications for mRNA and Peptide-Based VaccinesGuglielmo Lucchese0Hamid Reza Jahantigh1Leonarda De Benedictis2Piero Lovreglio3Angela Stufano4Department of Neurology, Medical University of Greifswald, 17475 Greifswald, GermanyInterdisciplinary Department of Medicine-Section of Occupational Medicine, University of Bari, 70124 Bari, ItalyInterdisciplinary Department of Medicine-Section of Occupational Medicine, University of Bari, 70124 Bari, ItalyInterdisciplinary Department of Medicine-Section of Occupational Medicine, University of Bari, 70124 Bari, ItalyInterdisciplinary Department of Medicine-Section of Occupational Medicine, University of Bari, 70124 Bari, ItalyHuman T-cell lymphotropic virus type 1 (HTLV-1) infection affects millions of individuals worldwide and can lead to severe leukemia, myelopathy/tropical spastic paraparesis, and numerous other disorders. Pursuing a safe and effective immunotherapeutic approach, we compared the viral polyprotein and the human proteome with a sliding window approach in order to identify oligopeptide sequences unique to the virus. The immunological relevance of the viral unique oligopeptides was assessed by searching them in the immune epitope database (IEDB). We found that HTLV-1 has 15 peptide stretches each consisting of uniquely viral non-human pentapeptides which are ideal candidate for a safe and effective anti-HTLV-1 vaccine. Indeed, experimentally validated HTLV-1 epitopes, as retrieved from the IEDB, contain peptide sequences also present in a vast number of human proteins, thus potentially instituting the basis for cross-reactions. We found a potential for cross-reactivity between the virus and the human proteome and described an epitope platform to be used in order to avoid it, thus obtaining effective, specific, and safe immunization. Potential advantages for mRNA and peptide-based vaccine formulations are discussed.https://www.mdpi.com/1999-4915/13/8/1461HTLV-1vaccinepeptideadjuvantsimilarityautoimmunity |
spellingShingle | Guglielmo Lucchese Hamid Reza Jahantigh Leonarda De Benedictis Piero Lovreglio Angela Stufano An Epitope Platform for Safe and Effective HTLV-1-Immunization: Potential Applications for mRNA and Peptide-Based Vaccines Viruses HTLV-1 vaccine peptide adjuvant similarity autoimmunity |
title | An Epitope Platform for Safe and Effective HTLV-1-Immunization: Potential Applications for mRNA and Peptide-Based Vaccines |
title_full | An Epitope Platform for Safe and Effective HTLV-1-Immunization: Potential Applications for mRNA and Peptide-Based Vaccines |
title_fullStr | An Epitope Platform for Safe and Effective HTLV-1-Immunization: Potential Applications for mRNA and Peptide-Based Vaccines |
title_full_unstemmed | An Epitope Platform for Safe and Effective HTLV-1-Immunization: Potential Applications for mRNA and Peptide-Based Vaccines |
title_short | An Epitope Platform for Safe and Effective HTLV-1-Immunization: Potential Applications for mRNA and Peptide-Based Vaccines |
title_sort | epitope platform for safe and effective htlv 1 immunization potential applications for mrna and peptide based vaccines |
topic | HTLV-1 vaccine peptide adjuvant similarity autoimmunity |
url | https://www.mdpi.com/1999-4915/13/8/1461 |
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