Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F m...

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Bibliographic Details
Main Authors: Tshiphiri Senamela, Marleen Kock, Piet Becker, Joachim J.C. Potgieter
Format: Article
Language:English
Published: AOSIS 2018-01-01
Series:African Journal of Laboratory Medicine
Subjects:
Janus kinase 2
locked nucleic acid
Myeloproliferative neoplasms
Polymerase chain reaction
Polycythaemia vera
Online Access:https://ajlmonline.org/index.php/ajlm/article/view/658

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