Characterisation of SNP haplotype structure in chemokine and chemokine receptor genes using CEPH pedigrees and statistical estimation
<p>Abstract</p> <p>Chemokine signals and their cell-surface receptors are important modulators of HIV-1 disease and cancer. To aid future case/control association studies, aim to further characterise the haplotype structure of variation in chemokine and chemokine receptor genes. To...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2004-03-01
|
| Series: | Human Genomics |
| Subjects: | |
| Online Access: | http://www.humgenomics.com/content/1/3/195 |
| _version_ | 1811184872994111488 |
|---|---|
| author | Clark Vanessa J Dean Michael |
| author_facet | Clark Vanessa J Dean Michael |
| author_sort | Clark Vanessa J |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Chemokine signals and their cell-surface receptors are important modulators of HIV-1 disease and cancer. To aid future case/control association studies, aim to further characterise the haplotype structure of variation in chemokine and chemokine receptor genes. To perform haplotype analysis in a population-based association study, haplotypes must be determined by estimation, in the absence of family information or laboratory methods to establish phase. Here, test the accuracy of estimates of haplotype frequency and linkage disequilibrium by comparing estimated haplotypes generated with the expectation maximisation (EM) algorithm to haplotypes determined from Centre d'Etude Polymorphisme Humain (CEPH) pedigree data. To do this, they have characterised haplotypes comprising alleles at 11 biallelic loci in four chemokine receptor genes (<it>CCR3</it>, <it>CCR2</it>, <it>CCR5 </it>and <it>CCRL2</it>), which span 150 kb on chromosome 3p21, and haplotyes of nine biallelic loci in six chemokine genes [<it>MCP</it>-<it>1(CCL2)</it>, <it>Eotaxin(CCL11), RANTES(CCL5)</it>, <it>MPIF-1(CCL23)</it>, <it>PARC(CCL18) </it>and <it>MIP-1α(CCL3) </it>] on chromosome 17q11-12. Forty multi-generation CEPH families, totalling 489 individuals, were genotyped by the TaqMan 5'-nuclease assay. Phased haplotypes and haplotypes estimated from unphased genotypes were compared in 103 grandparents who were assumed to have mated at random.</p> <p>For the 3p21 single nucleotide polymorphism (SNP) data, haplotypes determined by pedigree analysis and haplotypes generated by the EM algorithm were nearly identical. Linkage disequilibrium, measured by the D' statistic, was nearly maximal across the 150 kb region, with complete disequilibrium maintained at the extremes between <it>CCR3</it>-Y17Y and <it>CCRL2</it>-1243V. D'-values calculated from estimated haplotypes on 3p21 had high concordance with pairwise comparisons between pedigree-phased chromosomes. Conversely, there was less agreement between analyses of haplotype frequencies and linkage disequilibrium using estimated haplotypes when compared with pedigree-phased haplotypes of SNPs on chromosome 17q11-12. These results suggest that, while estimations of haplotype frequency and linkage disequilibrium may be relatively simple in the 3p21 chemokine receptor cluster in population samples, the more complex environment on chromosome 17q11-12 will require a higher resolution haplotype analysis.</p> |
| first_indexed | 2024-04-11T13:19:45Z |
| format | Article |
| id | doaj.art-e9e78093f0614c80ae697a49524c27a1 |
| institution | Directory Open Access Journal |
| issn | 1479-7364 |
| language | English |
| last_indexed | 2024-04-11T13:19:45Z |
| publishDate | 2004-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Human Genomics |
| spelling | doaj.art-e9e78093f0614c80ae697a49524c27a12022-12-22T04:22:15ZengBMCHuman Genomics1479-73642004-03-011319520710.1186/1479-7364-1-3-195Characterisation of SNP haplotype structure in chemokine and chemokine receptor genes using CEPH pedigrees and statistical estimationClark Vanessa JDean Michael<p>Abstract</p> <p>Chemokine signals and their cell-surface receptors are important modulators of HIV-1 disease and cancer. To aid future case/control association studies, aim to further characterise the haplotype structure of variation in chemokine and chemokine receptor genes. To perform haplotype analysis in a population-based association study, haplotypes must be determined by estimation, in the absence of family information or laboratory methods to establish phase. Here, test the accuracy of estimates of haplotype frequency and linkage disequilibrium by comparing estimated haplotypes generated with the expectation maximisation (EM) algorithm to haplotypes determined from Centre d'Etude Polymorphisme Humain (CEPH) pedigree data. To do this, they have characterised haplotypes comprising alleles at 11 biallelic loci in four chemokine receptor genes (<it>CCR3</it>, <it>CCR2</it>, <it>CCR5 </it>and <it>CCRL2</it>), which span 150 kb on chromosome 3p21, and haplotyes of nine biallelic loci in six chemokine genes [<it>MCP</it>-<it>1(CCL2)</it>, <it>Eotaxin(CCL11), RANTES(CCL5)</it>, <it>MPIF-1(CCL23)</it>, <it>PARC(CCL18) </it>and <it>MIP-1α(CCL3) </it>] on chromosome 17q11-12. Forty multi-generation CEPH families, totalling 489 individuals, were genotyped by the TaqMan 5'-nuclease assay. Phased haplotypes and haplotypes estimated from unphased genotypes were compared in 103 grandparents who were assumed to have mated at random.</p> <p>For the 3p21 single nucleotide polymorphism (SNP) data, haplotypes determined by pedigree analysis and haplotypes generated by the EM algorithm were nearly identical. Linkage disequilibrium, measured by the D' statistic, was nearly maximal across the 150 kb region, with complete disequilibrium maintained at the extremes between <it>CCR3</it>-Y17Y and <it>CCRL2</it>-1243V. D'-values calculated from estimated haplotypes on 3p21 had high concordance with pairwise comparisons between pedigree-phased chromosomes. Conversely, there was less agreement between analyses of haplotype frequencies and linkage disequilibrium using estimated haplotypes when compared with pedigree-phased haplotypes of SNPs on chromosome 17q11-12. These results suggest that, while estimations of haplotype frequency and linkage disequilibrium may be relatively simple in the 3p21 chemokine receptor cluster in population samples, the more complex environment on chromosome 17q11-12 will require a higher resolution haplotype analysis.</p>http://www.humgenomics.com/content/1/3/195chemokineSNPhaplotype estimationpedigree analysislinkage disequilibrium |
| spellingShingle | Clark Vanessa J Dean Michael Characterisation of SNP haplotype structure in chemokine and chemokine receptor genes using CEPH pedigrees and statistical estimation Human Genomics chemokine SNP haplotype estimation pedigree analysis linkage disequilibrium |
| title | Characterisation of SNP haplotype structure in chemokine and chemokine receptor genes using CEPH pedigrees and statistical estimation |
| title_full | Characterisation of SNP haplotype structure in chemokine and chemokine receptor genes using CEPH pedigrees and statistical estimation |
| title_fullStr | Characterisation of SNP haplotype structure in chemokine and chemokine receptor genes using CEPH pedigrees and statistical estimation |
| title_full_unstemmed | Characterisation of SNP haplotype structure in chemokine and chemokine receptor genes using CEPH pedigrees and statistical estimation |
| title_short | Characterisation of SNP haplotype structure in chemokine and chemokine receptor genes using CEPH pedigrees and statistical estimation |
| title_sort | characterisation of snp haplotype structure in chemokine and chemokine receptor genes using ceph pedigrees and statistical estimation |
| topic | chemokine SNP haplotype estimation pedigree analysis linkage disequilibrium |
| url | http://www.humgenomics.com/content/1/3/195 |
| work_keys_str_mv | AT clarkvanessaj characterisationofsnphaplotypestructureinchemokineandchemokinereceptorgenesusingcephpedigreesandstatisticalestimation AT deanmichael characterisationofsnphaplotypestructureinchemokineandchemokinereceptorgenesusingcephpedigreesandstatisticalestimation |