Development of a Rapid, Antimicrobial Susceptibility Test for <i>E. coli</i> Based on Low-Cost, Screen-Printed Electrodes
Antibiotic resistance has been cited by the World Health Organisation (WHO) as one of the greatest threats to public health. Mitigating the spread of antibiotic resistance requires a multipronged approach with possible interventions including faster diagnostic testing and enhanced antibiotic steward...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/10/11/153 |
| _version_ | 1797550063950495744 |
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| author | Stuart Hannah Alexandra Dobrea Perrine Lasserre Ewen O. Blair David Alcorn Paul A. Hoskisson Damion K. Corrigan |
| author_facet | Stuart Hannah Alexandra Dobrea Perrine Lasserre Ewen O. Blair David Alcorn Paul A. Hoskisson Damion K. Corrigan |
| author_sort | Stuart Hannah |
| collection | DOAJ |
| description | Antibiotic resistance has been cited by the World Health Organisation (WHO) as one of the greatest threats to public health. Mitigating the spread of antibiotic resistance requires a multipronged approach with possible interventions including faster diagnostic testing and enhanced antibiotic stewardship. This study employs a low-cost diagnostic sensor test to rapidly pinpoint the correct antibiotic for treatment of infection. The sensor comprises a screen-printed gold electrode, modified with an antibiotic-seeded hydrogel to monitor bacterial growth. Electrochemical growth profiles of the common microorganism, <i>Escherichia coli</i> (<i>E. coli</i>) (ATCC 25922) were measured in the presence and absence of the antibiotic streptomycin. Results show a clear distinction between the <i>E. coli</i> growth profiles depending on whether streptomycin is present, in a timeframe of ≈2.5 h (<i>p</i> < 0.05), significantly quicker than the current gold standard of culture-based antimicrobial susceptibility testing. These results demonstrate a clear pathway to a low cost, phenotypic and reproducible antibiotic susceptibility testing technology for the rapid detection of <i>E. coli</i> within clinically relevant concentration ranges for conditions such as urinary tract infections. |
| first_indexed | 2024-03-10T15:23:09Z |
| format | Article |
| id | doaj.art-e9ea70fa618c41c7b20c96b3edbb39d8 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T15:23:09Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-e9ea70fa618c41c7b20c96b3edbb39d82023-11-20T18:18:47ZengMDPI AGBiosensors2079-63742020-10-01101115310.3390/bios10110153Development of a Rapid, Antimicrobial Susceptibility Test for <i>E. coli</i> Based on Low-Cost, Screen-Printed ElectrodesStuart Hannah0Alexandra Dobrea1Perrine Lasserre2Ewen O. Blair3David Alcorn4Paul A. Hoskisson5Damion K. Corrigan6Department of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, UKDepartment of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, UKDepartment of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, UKDepartment of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, UKDivision of Anaesthesia, Royal Alexandra Hospital, Corsebar Road, Paisley PA2 9PN, UKStrathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 161 Cathedral Street, Glasgow G4 0RE, UKDepartment of Biomedical Engineering, University of Strathclyde, 40 George Street, Glasgow G1 1QE, UKAntibiotic resistance has been cited by the World Health Organisation (WHO) as one of the greatest threats to public health. Mitigating the spread of antibiotic resistance requires a multipronged approach with possible interventions including faster diagnostic testing and enhanced antibiotic stewardship. This study employs a low-cost diagnostic sensor test to rapidly pinpoint the correct antibiotic for treatment of infection. The sensor comprises a screen-printed gold electrode, modified with an antibiotic-seeded hydrogel to monitor bacterial growth. Electrochemical growth profiles of the common microorganism, <i>Escherichia coli</i> (<i>E. coli</i>) (ATCC 25922) were measured in the presence and absence of the antibiotic streptomycin. Results show a clear distinction between the <i>E. coli</i> growth profiles depending on whether streptomycin is present, in a timeframe of ≈2.5 h (<i>p</i> < 0.05), significantly quicker than the current gold standard of culture-based antimicrobial susceptibility testing. These results demonstrate a clear pathway to a low cost, phenotypic and reproducible antibiotic susceptibility testing technology for the rapid detection of <i>E. coli</i> within clinically relevant concentration ranges for conditions such as urinary tract infections.https://www.mdpi.com/2079-6374/10/11/153antibiotic susceptibility testing (AST)<i>Escherichia coli</i> (<i>E. coli)</i>electrochemistryscreen-printed electrodes (SPEs)streptomycingrowth-profiles |
| spellingShingle | Stuart Hannah Alexandra Dobrea Perrine Lasserre Ewen O. Blair David Alcorn Paul A. Hoskisson Damion K. Corrigan Development of a Rapid, Antimicrobial Susceptibility Test for <i>E. coli</i> Based on Low-Cost, Screen-Printed Electrodes Biosensors antibiotic susceptibility testing (AST) <i>Escherichia coli</i> (<i>E. coli)</i> electrochemistry screen-printed electrodes (SPEs) streptomycin growth-profiles |
| title | Development of a Rapid, Antimicrobial Susceptibility Test for <i>E. coli</i> Based on Low-Cost, Screen-Printed Electrodes |
| title_full | Development of a Rapid, Antimicrobial Susceptibility Test for <i>E. coli</i> Based on Low-Cost, Screen-Printed Electrodes |
| title_fullStr | Development of a Rapid, Antimicrobial Susceptibility Test for <i>E. coli</i> Based on Low-Cost, Screen-Printed Electrodes |
| title_full_unstemmed | Development of a Rapid, Antimicrobial Susceptibility Test for <i>E. coli</i> Based on Low-Cost, Screen-Printed Electrodes |
| title_short | Development of a Rapid, Antimicrobial Susceptibility Test for <i>E. coli</i> Based on Low-Cost, Screen-Printed Electrodes |
| title_sort | development of a rapid antimicrobial susceptibility test for i e coli i based on low cost screen printed electrodes |
| topic | antibiotic susceptibility testing (AST) <i>Escherichia coli</i> (<i>E. coli)</i> electrochemistry screen-printed electrodes (SPEs) streptomycin growth-profiles |
| url | https://www.mdpi.com/2079-6374/10/11/153 |
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