Antimicrobial Activity of Olea europaea Fatty Oil against Multi-Drug Resistant and Biofilm Forming Microorganisms
The aim of the present study was to investigate the potential antimicrobial activity of Olea europaea fatty oil against a collection of bacterial (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) and fungal (Candida albicans) clinical isolates. The antimicrobial and antibiofilm ac...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2018-12-01
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| Series: | Notulae Scientia Biologicae |
| Subjects: | |
| Online Access: | https://www.notulaebiologicae.ro/index.php/nsb/article/view/10404 |
| _version_ | 1811220248060231680 |
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| author | Haifaa Bawie NAJEE Dunya ALKURJIA Othman ALMAHDAWY Crina KAMERZAN Luminita MARUTESCU Irina GHEORGHE Marcela POPA Mariana Carmen CHIFIRIUC Veronica LAZĂR |
| author_facet | Haifaa Bawie NAJEE Dunya ALKURJIA Othman ALMAHDAWY Crina KAMERZAN Luminita MARUTESCU Irina GHEORGHE Marcela POPA Mariana Carmen CHIFIRIUC Veronica LAZĂR |
| author_sort | Haifaa Bawie NAJEE |
| collection | DOAJ |
| description | The aim of the present study was to investigate the potential antimicrobial activity of Olea europaea fatty oil against a collection of bacterial (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) and fungal (Candida albicans) clinical isolates. The antimicrobial and antibiofilm activity were evaluated by broth microdilution method for establishing the minimal inhibitory concentration (MIC) and microtiter assay for determining the minimal biofilm eradication concentration (MBEC). Some of the potential microbial targets of the fatty oil were investigated by flow cytometry (FCM). The results obtained hereby revealed that Olea europaea fatty oil inhibited microbial planktonic growth (MICs values of 5.23-41.8 mg/ mL) and biofilm development on inert substrata (MBEC values of 1.31-20.9 mg/ mL). The FCM measurements confirmed that the analyzed oil induced microbial membrane damages and inhibited microbial efflux pump activity. |
| first_indexed | 2024-04-12T07:38:29Z |
| format | Article |
| id | doaj.art-e9eb394a78a844c186068fb140760d77 |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-04-12T07:38:29Z |
| publishDate | 2018-12-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-e9eb394a78a844c186068fb140760d772022-12-22T03:41:53ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642018-12-0110449850210.15835/nsb104104048428Antimicrobial Activity of Olea europaea Fatty Oil against Multi-Drug Resistant and Biofilm Forming MicroorganismsHaifaa Bawie NAJEE0Dunya ALKURJIA1Othman ALMAHDAWY2Crina KAMERZAN3Luminita MARUTESCU4Irina GHEORGHE5Marcela POPA6Mariana Carmen CHIFIRIUC7Veronica LAZĂR8University of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUBUniversity of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUBUniversity of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUBResearch Institute of the University of Bucharest-ICUBUniversity of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUBUniversity of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUBUniversity of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUBUniversity of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUBUniversity of Bucharest, Faculty of Biology, Department of Botany-Microbiology, Research Institute of the University of Bucharest-ICUBThe aim of the present study was to investigate the potential antimicrobial activity of Olea europaea fatty oil against a collection of bacterial (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) and fungal (Candida albicans) clinical isolates. The antimicrobial and antibiofilm activity were evaluated by broth microdilution method for establishing the minimal inhibitory concentration (MIC) and microtiter assay for determining the minimal biofilm eradication concentration (MBEC). Some of the potential microbial targets of the fatty oil were investigated by flow cytometry (FCM). The results obtained hereby revealed that Olea europaea fatty oil inhibited microbial planktonic growth (MICs values of 5.23-41.8 mg/ mL) and biofilm development on inert substrata (MBEC values of 1.31-20.9 mg/ mL). The FCM measurements confirmed that the analyzed oil induced microbial membrane damages and inhibited microbial efflux pump activity.https://www.notulaebiologicae.ro/index.php/nsb/article/view/10404antimicrobial activityanti-biofilm activityefflux pumpfatty oilflow cytometry |
| spellingShingle | Haifaa Bawie NAJEE Dunya ALKURJIA Othman ALMAHDAWY Crina KAMERZAN Luminita MARUTESCU Irina GHEORGHE Marcela POPA Mariana Carmen CHIFIRIUC Veronica LAZĂR Antimicrobial Activity of Olea europaea Fatty Oil against Multi-Drug Resistant and Biofilm Forming Microorganisms Notulae Scientia Biologicae antimicrobial activity anti-biofilm activity efflux pump fatty oil flow cytometry |
| title | Antimicrobial Activity of Olea europaea Fatty Oil against Multi-Drug Resistant and Biofilm Forming Microorganisms |
| title_full | Antimicrobial Activity of Olea europaea Fatty Oil against Multi-Drug Resistant and Biofilm Forming Microorganisms |
| title_fullStr | Antimicrobial Activity of Olea europaea Fatty Oil against Multi-Drug Resistant and Biofilm Forming Microorganisms |
| title_full_unstemmed | Antimicrobial Activity of Olea europaea Fatty Oil against Multi-Drug Resistant and Biofilm Forming Microorganisms |
| title_short | Antimicrobial Activity of Olea europaea Fatty Oil against Multi-Drug Resistant and Biofilm Forming Microorganisms |
| title_sort | antimicrobial activity of olea europaea fatty oil against multi drug resistant and biofilm forming microorganisms |
| topic | antimicrobial activity anti-biofilm activity efflux pump fatty oil flow cytometry |
| url | https://www.notulaebiologicae.ro/index.php/nsb/article/view/10404 |
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