Rhizosphere P-Enzyme Activity, Mineral Nutrient Concentrations, and Microbial Community Structure Are Altered by Intra-Hole Cropping of Cowpea With Cereals
In Africa, intercropping and intra-hole cropping systems are common practices used by smallholder farmers to optimize land use and tap the benefits of plant-to-plant interactions. The aim of this study was to evaluate mineral nutrient concentrations, P-enzyme activity and changes in microbial commun...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Agronomy |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fagro.2021.666351/full |
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| author | Sanjay K. Jaiswal Mpelang P. Maredi Felix D. Dakora |
| author_facet | Sanjay K. Jaiswal Mpelang P. Maredi Felix D. Dakora |
| author_sort | Sanjay K. Jaiswal |
| collection | DOAJ |
| description | In Africa, intercropping and intra-hole cropping systems are common practices used by smallholder farmers to optimize land use and tap the benefits of plant-to-plant interactions. The aim of this study was to evaluate mineral nutrient concentrations, P-enzyme activity and changes in microbial communities in the rhizospheres of sole cropped and intra-hole planted cowpea (cvs. TVu 546 and PAN 311), maize (cv. ZM 521), and sorghum (cv. M48). Cowpea cv. TVu546 intra-hole planted with sorghum (i.e., TVu546+M48) produced the highest rhizosphere acid phosphatase (APase) activity (230.0 μg p-nitrophenol.g−1 soil.h−1). From 16S rRNA Miseq Illumina sequencing, the rhizosphere bacterial community structure was altered by intra-hole cropping, and was dominated by Actinobacteria, Acidobacteria, Bacteroidetes, Firmicutes, Planctomycetes, Proteobacteria, and Verrucomicrobia, which together accounted for about >95% of the total sequences. The Sphingobacteria phylum was the dominant microbial group in the rhizosphere soil of all the cropping systems. The Proteobacteria phylum was the second most abundant in this study, which included the beneficial bacteria in all the rhizosphere soils studied. In contrast, typical pathogens like Ralsotonia and Agrobacterium were completely absent, indicating that the intra-hole cropping system can provide protection against soil-borne diseases possibly through elimination by antibiotics and/or phytoalexins present in plant and microbial exudates in the rhizosphere. Mucilaginibacter and Flavobacterium were however selectively present with intra-hole cropping. |
| first_indexed | 2024-12-22T06:09:34Z |
| format | Article |
| id | doaj.art-e9f1e3e2c54440439a3d403ad7be7600 |
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| issn | 2673-3218 |
| language | English |
| last_indexed | 2024-12-22T06:09:34Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Agronomy |
| spelling | doaj.art-e9f1e3e2c54440439a3d403ad7be76002022-12-21T18:36:17ZengFrontiers Media S.A.Frontiers in Agronomy2673-32182021-07-01310.3389/fagro.2021.666351666351Rhizosphere P-Enzyme Activity, Mineral Nutrient Concentrations, and Microbial Community Structure Are Altered by Intra-Hole Cropping of Cowpea With CerealsSanjay K. Jaiswal0Mpelang P. Maredi1Felix D. Dakora2Department of Chemistry, Tshwane University of Technology, Pretoria, South AfricaDepartment of Crop Sciences, Tshwane University of Technology, Pretoria, South AfricaDepartment of Chemistry, Tshwane University of Technology, Pretoria, South AfricaIn Africa, intercropping and intra-hole cropping systems are common practices used by smallholder farmers to optimize land use and tap the benefits of plant-to-plant interactions. The aim of this study was to evaluate mineral nutrient concentrations, P-enzyme activity and changes in microbial communities in the rhizospheres of sole cropped and intra-hole planted cowpea (cvs. TVu 546 and PAN 311), maize (cv. ZM 521), and sorghum (cv. M48). Cowpea cv. TVu546 intra-hole planted with sorghum (i.e., TVu546+M48) produced the highest rhizosphere acid phosphatase (APase) activity (230.0 μg p-nitrophenol.g−1 soil.h−1). From 16S rRNA Miseq Illumina sequencing, the rhizosphere bacterial community structure was altered by intra-hole cropping, and was dominated by Actinobacteria, Acidobacteria, Bacteroidetes, Firmicutes, Planctomycetes, Proteobacteria, and Verrucomicrobia, which together accounted for about >95% of the total sequences. The Sphingobacteria phylum was the dominant microbial group in the rhizosphere soil of all the cropping systems. The Proteobacteria phylum was the second most abundant in this study, which included the beneficial bacteria in all the rhizosphere soils studied. In contrast, typical pathogens like Ralsotonia and Agrobacterium were completely absent, indicating that the intra-hole cropping system can provide protection against soil-borne diseases possibly through elimination by antibiotics and/or phytoalexins present in plant and microbial exudates in the rhizosphere. Mucilaginibacter and Flavobacterium were however selectively present with intra-hole cropping.https://www.frontiersin.org/articles/10.3389/fagro.2021.666351/fullintra-hole and sole croppingacid phosphatase activitymicrobial community16S rRNAMiseq Illumina sequencing |
| spellingShingle | Sanjay K. Jaiswal Mpelang P. Maredi Felix D. Dakora Rhizosphere P-Enzyme Activity, Mineral Nutrient Concentrations, and Microbial Community Structure Are Altered by Intra-Hole Cropping of Cowpea With Cereals Frontiers in Agronomy intra-hole and sole cropping acid phosphatase activity microbial community 16S rRNA Miseq Illumina sequencing |
| title | Rhizosphere P-Enzyme Activity, Mineral Nutrient Concentrations, and Microbial Community Structure Are Altered by Intra-Hole Cropping of Cowpea With Cereals |
| title_full | Rhizosphere P-Enzyme Activity, Mineral Nutrient Concentrations, and Microbial Community Structure Are Altered by Intra-Hole Cropping of Cowpea With Cereals |
| title_fullStr | Rhizosphere P-Enzyme Activity, Mineral Nutrient Concentrations, and Microbial Community Structure Are Altered by Intra-Hole Cropping of Cowpea With Cereals |
| title_full_unstemmed | Rhizosphere P-Enzyme Activity, Mineral Nutrient Concentrations, and Microbial Community Structure Are Altered by Intra-Hole Cropping of Cowpea With Cereals |
| title_short | Rhizosphere P-Enzyme Activity, Mineral Nutrient Concentrations, and Microbial Community Structure Are Altered by Intra-Hole Cropping of Cowpea With Cereals |
| title_sort | rhizosphere p enzyme activity mineral nutrient concentrations and microbial community structure are altered by intra hole cropping of cowpea with cereals |
| topic | intra-hole and sole cropping acid phosphatase activity microbial community 16S rRNA Miseq Illumina sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fagro.2021.666351/full |
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