<i>Qipengyuania pacifica</i> sp. nov., a Novel Carotenoid-Producing Marine Bacterium of the Family <i>Erythrobacteraceae</i>, Isolated from Sponge (<i>Demospongiae</i>), and Antimicrobial Potential of Its Crude Extract
A marine <i>Alphaproteobacterium</i> designated as strain NZ-96<sup>T</sup> was isolated in February 2021, from a sponge species (<i>Demospongiae</i>) collected in muddy sediments with boulders and old chimneys in Otago/Canterbury Slope, Pacific Ocean, New Zealand...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Diversity |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-2818/14/4/295 |
| _version_ | 1797436028947005440 |
|---|---|
| author | Sanaullah Tareen Chandra Risdian Mathias Müsken Joachim Wink |
| author_facet | Sanaullah Tareen Chandra Risdian Mathias Müsken Joachim Wink |
| author_sort | Sanaullah Tareen |
| collection | DOAJ |
| description | A marine <i>Alphaproteobacterium</i> designated as strain NZ-96<sup>T</sup> was isolated in February 2021, from a sponge species (<i>Demospongiae</i>) collected in muddy sediments with boulders and old chimneys in Otago/Canterbury Slope, Pacific Ocean, New Zealand. The isolate was found to be Gram-negative, rod-shaped, aerobic, motile, and produced yellow-colored colonies. The isolate was positive for alkaline phosphatase, leucine arylamidase, trypsin, catalase, and oxidase and negative for α-galactosidase and urease. It was resistant to many antibiotics including hygromycin, trimethoprim, spectinomycin, ampicillin, oxytetracycline, cephalosporin, bacitracin, and polymyxin. The 16S rRNA gene-based phylogenetic analyses exhibited that strain NZ-96<sup>T</sup> belonged to the genus <i>Qipengyuania</i> and showed 98.3–98.8% 16S rRNA gene sequence similarity to its closest relatives. The major respiratory quinone was ubiquinone-10 (Q-10). The polar lipid profile consisted of phosphatidylcholine, sphingoglycolipid, phosphatidylglycerol, one unknown polar lipid, and three unknown glycolipids. The major fatty acids were C<sub>18:1</sub>ω12t, C<sub>16:0</sub>, C<sub>16:1</sub>ω7c, C<sub>17:1</sub>ω6c, C<sub>16:0</sub>2-OH, and C<sub>14:0</sub> 2-OH. Carotenoid were produced. The crude extract showed pronounced activity against <i>Staphylococcus aureus</i> Newman and <i>Bacillus subtilis</i> DSM 10. Pairwise ANI and dDDH values of strain NZ-96<sup>T</sup> and closely related phylogenetic hits were below the threshold values of 95% and 70%, respectively. Genes for trehalose biosynthesis, aspartate-semialdehyde dehydrogenase, flagellar biosynthesis, fatty acid biosynthesis, and antibiotics resistance were present, which aids in isolate survival in a sea or ocean environment. The DNA G+C content was 60.8% (by genome). Based on data obtained by the polyphasic approach, strain NZ-96<sup>T</sup> (= DSM 112811<sup>T</sup> = NCCB 100842<sup>T</sup>) represents a novel species of the genus <i>Qipengyuania</i>, for which the name <i>Qipengyuania pacifica</i> sp. nov. is proposed. |
| first_indexed | 2024-03-09T10:56:46Z |
| format | Article |
| id | doaj.art-e9f3250ef5324c85b356aac75783d898 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-09T10:56:46Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-e9f3250ef5324c85b356aac75783d8982023-12-01T01:37:43ZengMDPI AGDiversity1424-28182022-04-0114429510.3390/d14040295<i>Qipengyuania pacifica</i> sp. nov., a Novel Carotenoid-Producing Marine Bacterium of the Family <i>Erythrobacteraceae</i>, Isolated from Sponge (<i>Demospongiae</i>), and Antimicrobial Potential of Its Crude ExtractSanaullah Tareen0Chandra Risdian1Mathias Müsken2Joachim Wink3Microbial Strain Collection (MISG), Helmholtz Centre for Infection Research (HZI), 38124 Braunschweig, GermanyMicrobial Strain Collection (MISG), Helmholtz Centre for Infection Research (HZI), 38124 Braunschweig, GermanyCentral Facility for Microscopy, Helmholtz Centre for Infection Research (HZI), 38124 Braunschweig, GermanyMicrobial Strain Collection (MISG), Helmholtz Centre for Infection Research (HZI), 38124 Braunschweig, GermanyA marine <i>Alphaproteobacterium</i> designated as strain NZ-96<sup>T</sup> was isolated in February 2021, from a sponge species (<i>Demospongiae</i>) collected in muddy sediments with boulders and old chimneys in Otago/Canterbury Slope, Pacific Ocean, New Zealand. The isolate was found to be Gram-negative, rod-shaped, aerobic, motile, and produced yellow-colored colonies. The isolate was positive for alkaline phosphatase, leucine arylamidase, trypsin, catalase, and oxidase and negative for α-galactosidase and urease. It was resistant to many antibiotics including hygromycin, trimethoprim, spectinomycin, ampicillin, oxytetracycline, cephalosporin, bacitracin, and polymyxin. The 16S rRNA gene-based phylogenetic analyses exhibited that strain NZ-96<sup>T</sup> belonged to the genus <i>Qipengyuania</i> and showed 98.3–98.8% 16S rRNA gene sequence similarity to its closest relatives. The major respiratory quinone was ubiquinone-10 (Q-10). The polar lipid profile consisted of phosphatidylcholine, sphingoglycolipid, phosphatidylglycerol, one unknown polar lipid, and three unknown glycolipids. The major fatty acids were C<sub>18:1</sub>ω12t, C<sub>16:0</sub>, C<sub>16:1</sub>ω7c, C<sub>17:1</sub>ω6c, C<sub>16:0</sub>2-OH, and C<sub>14:0</sub> 2-OH. Carotenoid were produced. The crude extract showed pronounced activity against <i>Staphylococcus aureus</i> Newman and <i>Bacillus subtilis</i> DSM 10. Pairwise ANI and dDDH values of strain NZ-96<sup>T</sup> and closely related phylogenetic hits were below the threshold values of 95% and 70%, respectively. Genes for trehalose biosynthesis, aspartate-semialdehyde dehydrogenase, flagellar biosynthesis, fatty acid biosynthesis, and antibiotics resistance were present, which aids in isolate survival in a sea or ocean environment. The DNA G+C content was 60.8% (by genome). Based on data obtained by the polyphasic approach, strain NZ-96<sup>T</sup> (= DSM 112811<sup>T</sup> = NCCB 100842<sup>T</sup>) represents a novel species of the genus <i>Qipengyuania</i>, for which the name <i>Qipengyuania pacifica</i> sp. nov. is proposed.https://www.mdpi.com/1424-2818/14/4/295carotenoid<i>Erythrobacteraceae</i>Pacific Oceanpolyphasic analysissponge |
| spellingShingle | Sanaullah Tareen Chandra Risdian Mathias Müsken Joachim Wink <i>Qipengyuania pacifica</i> sp. nov., a Novel Carotenoid-Producing Marine Bacterium of the Family <i>Erythrobacteraceae</i>, Isolated from Sponge (<i>Demospongiae</i>), and Antimicrobial Potential of Its Crude Extract Diversity carotenoid <i>Erythrobacteraceae</i> Pacific Ocean polyphasic analysis sponge |
| title | <i>Qipengyuania pacifica</i> sp. nov., a Novel Carotenoid-Producing Marine Bacterium of the Family <i>Erythrobacteraceae</i>, Isolated from Sponge (<i>Demospongiae</i>), and Antimicrobial Potential of Its Crude Extract |
| title_full | <i>Qipengyuania pacifica</i> sp. nov., a Novel Carotenoid-Producing Marine Bacterium of the Family <i>Erythrobacteraceae</i>, Isolated from Sponge (<i>Demospongiae</i>), and Antimicrobial Potential of Its Crude Extract |
| title_fullStr | <i>Qipengyuania pacifica</i> sp. nov., a Novel Carotenoid-Producing Marine Bacterium of the Family <i>Erythrobacteraceae</i>, Isolated from Sponge (<i>Demospongiae</i>), and Antimicrobial Potential of Its Crude Extract |
| title_full_unstemmed | <i>Qipengyuania pacifica</i> sp. nov., a Novel Carotenoid-Producing Marine Bacterium of the Family <i>Erythrobacteraceae</i>, Isolated from Sponge (<i>Demospongiae</i>), and Antimicrobial Potential of Its Crude Extract |
| title_short | <i>Qipengyuania pacifica</i> sp. nov., a Novel Carotenoid-Producing Marine Bacterium of the Family <i>Erythrobacteraceae</i>, Isolated from Sponge (<i>Demospongiae</i>), and Antimicrobial Potential of Its Crude Extract |
| title_sort | i qipengyuania pacifica i sp nov a novel carotenoid producing marine bacterium of the family i erythrobacteraceae i isolated from sponge i demospongiae i and antimicrobial potential of its crude extract |
| topic | carotenoid <i>Erythrobacteraceae</i> Pacific Ocean polyphasic analysis sponge |
| url | https://www.mdpi.com/1424-2818/14/4/295 |
| work_keys_str_mv | AT sanaullahtareen iqipengyuaniapacificaispnovanovelcarotenoidproducingmarinebacteriumofthefamilyierythrobacteraceaeiisolatedfromspongeidemospongiaeiandantimicrobialpotentialofitscrudeextract AT chandrarisdian iqipengyuaniapacificaispnovanovelcarotenoidproducingmarinebacteriumofthefamilyierythrobacteraceaeiisolatedfromspongeidemospongiaeiandantimicrobialpotentialofitscrudeextract AT mathiasmusken iqipengyuaniapacificaispnovanovelcarotenoidproducingmarinebacteriumofthefamilyierythrobacteraceaeiisolatedfromspongeidemospongiaeiandantimicrobialpotentialofitscrudeextract AT joachimwink iqipengyuaniapacificaispnovanovelcarotenoidproducingmarinebacteriumofthefamilyierythrobacteraceaeiisolatedfromspongeidemospongiaeiandantimicrobialpotentialofitscrudeextract |