Testing the efficacy of different molecular tools for parasite conservation genetics: a case study using horsehair worms (Phylum: Nematomorpha)
In recent years, parasite conservation has become a globally significant issue. Because of this, there is a need for standardized methods for inferring population status and possible cryptic diversity. However, given the lack of molecular data for some groups, it is challenging to establish procedur...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2023-08-01
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| Series: | Parasitology |
| Subjects: | |
| Online Access: | https://www.cambridge.org/core/product/identifier/S0031182023000641/type/journal_article |
| _version_ | 1797741371455438848 |
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| author | Mattia De Vivo Wei-Yun Chen Jen-Pan Huang |
| author_facet | Mattia De Vivo Wei-Yun Chen Jen-Pan Huang |
| author_sort | Mattia De Vivo |
| collection | DOAJ |
| description | In recent years, parasite conservation has become a globally significant issue. Because of this, there is a need for standardized methods for inferring population status and possible cryptic diversity. However, given the lack of molecular data for some groups, it is challenging to establish procedures for genetic diversity estimation. Therefore, universal tools, such as double-digest restriction-site-associated DNA sequencing (ddRADseq), could be useful when conducting conservation genetic studies on rarely studied parasites. Here, we generated a ddRADseq dataset that includes all 3 described Taiwanese horsehair worms (Phylum: Nematomorpha), possibly one of the most understudied animal groups. Additionally, we produced data for a fragment of the cytochrome c oxidase subunit I (COXI) for the said species. We used the COXI dataset in combination with previously published sequences of the same locus for inferring the effective population size (Ne) trends and possible population genetic structure. |
| first_indexed | 2024-03-12T14:25:48Z |
| format | Article |
| id | doaj.art-e9f3759a804046a0bd6995744043826b |
| institution | Directory Open Access Journal |
| issn | 0031-1820 1469-8161 |
| language | English |
| last_indexed | 2024-03-12T14:25:48Z |
| publishDate | 2023-08-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Parasitology |
| spelling | doaj.art-e9f3759a804046a0bd6995744043826b2023-08-18T10:07:43ZengCambridge University PressParasitology0031-18201469-81612023-08-0115084285110.1017/S0031182023000641Testing the efficacy of different molecular tools for parasite conservation genetics: a case study using horsehair worms (Phylum: Nematomorpha)Mattia De Vivo0https://orcid.org/0000-0002-9115-1941Wei-Yun Chen1Jen-Pan Huang2Department of Life Science, National Taiwan Normal University, Taipei, Taiwan Biodiversity Program, Taiwan International Graduate Program, Taipei, Taiwan Biodiversity Research Center, Academia Sinica, Taipei, TaiwanBiodiversity Research Center, Academia Sinica, Taipei, TaiwanBiodiversity Research Center, Academia Sinica, Taipei, TaiwanIn recent years, parasite conservation has become a globally significant issue. Because of this, there is a need for standardized methods for inferring population status and possible cryptic diversity. However, given the lack of molecular data for some groups, it is challenging to establish procedures for genetic diversity estimation. Therefore, universal tools, such as double-digest restriction-site-associated DNA sequencing (ddRADseq), could be useful when conducting conservation genetic studies on rarely studied parasites. Here, we generated a ddRADseq dataset that includes all 3 described Taiwanese horsehair worms (Phylum: Nematomorpha), possibly one of the most understudied animal groups. Additionally, we produced data for a fragment of the cytochrome c oxidase subunit I (COXI) for the said species. We used the COXI dataset in combination with previously published sequences of the same locus for inferring the effective population size (Ne) trends and possible population genetic structure.https://www.cambridge.org/core/product/identifier/S0031182023000641/type/journal_articleCOXIddRADseqnematomorphaparasite conservationpopulation sizeTaiwan |
| spellingShingle | Mattia De Vivo Wei-Yun Chen Jen-Pan Huang Testing the efficacy of different molecular tools for parasite conservation genetics: a case study using horsehair worms (Phylum: Nematomorpha) Parasitology COXI ddRADseq nematomorpha parasite conservation population size Taiwan |
| title | Testing the efficacy of different molecular tools for parasite conservation genetics: a case study using horsehair worms (Phylum: Nematomorpha) |
| title_full | Testing the efficacy of different molecular tools for parasite conservation genetics: a case study using horsehair worms (Phylum: Nematomorpha) |
| title_fullStr | Testing the efficacy of different molecular tools for parasite conservation genetics: a case study using horsehair worms (Phylum: Nematomorpha) |
| title_full_unstemmed | Testing the efficacy of different molecular tools for parasite conservation genetics: a case study using horsehair worms (Phylum: Nematomorpha) |
| title_short | Testing the efficacy of different molecular tools for parasite conservation genetics: a case study using horsehair worms (Phylum: Nematomorpha) |
| title_sort | testing the efficacy of different molecular tools for parasite conservation genetics a case study using horsehair worms phylum nematomorpha |
| topic | COXI ddRADseq nematomorpha parasite conservation population size Taiwan |
| url | https://www.cambridge.org/core/product/identifier/S0031182023000641/type/journal_article |
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