Evaluation of floral volatile patterns in the genus Narcissus using gas chromatography–coupled ion mobility spectrometry
Abstract Premise Daffodils (Narcissus, Amaryllidaceae) are iconic ornamentals with a complex floral biology and many fragrant species; however, little is known about floral plant volatile organic compounds (pVOCs) across the genus and additional sampling is desirable. The present study investigates...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aps3.11506 |
| _version_ | 1797904666962427904 |
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| author | Florian Losch Sascha Liedtke Wolfgang Vautz Maximilian Weigend |
| author_facet | Florian Losch Sascha Liedtke Wolfgang Vautz Maximilian Weigend |
| author_sort | Florian Losch |
| collection | DOAJ |
| description | Abstract Premise Daffodils (Narcissus, Amaryllidaceae) are iconic ornamentals with a complex floral biology and many fragrant species; however, little is known about floral plant volatile organic compounds (pVOCs) across the genus and additional sampling is desirable. The present study investigates whether the floral scent of 20 species of Narcissus can be characterized using gas chromatography–coupled ion mobility spectrometry (GC–IMS), with the aim of building a comparative pVOC data set for ecological and evolutionary studies. Methods We used a commercial GC–IMS equipped with an integrated in‐line enrichment system for a fast, sensitive, and automated pVOC analysis. This facilitates qualitative and (semi)‐quantitative measurements without sample preparation. Results The GC–IMS provided detailed data on floral pVOCs in Narcissus with very short sampling times and without floral enclosure. A wide range of compounds was recorded and partially identified. The retrieved pVOC patterns showed a good agreement with published data, and five “chemotypes” were characterized as characteristic combinations of floral volatiles. Discussion The GC–IMS setup can be applied to rapidly generate large amounts of pVOC data with high sensitivity and selectivity. The preliminary data on Narcissus obtained here indicate both considerable pVOC variability and a good correspondence of the pVOC patterns with infrageneric classification, supporting the hypothesis that floral scent could represent a considerable phylogenetic signal. |
| first_indexed | 2024-04-10T09:52:36Z |
| format | Article |
| id | doaj.art-e9f88515e41a4fee8233d0472e3b681c |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-04-10T09:52:36Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-e9f88515e41a4fee8233d0472e3b681c2023-02-16T18:44:42ZengWileyApplications in Plant Sciences2168-04502023-01-01111n/an/a10.1002/aps3.11506Evaluation of floral volatile patterns in the genus Narcissus using gas chromatography–coupled ion mobility spectrometryFlorian Losch0Sascha Liedtke1Wolfgang Vautz2Maximilian Weigend3Nees‐Institut für Biodiversität der Pflanzen, Mathematisch‐Naturwissenschaftliche Fakultät Rheinische Friedrich‐Wilhelmsuniversität Bonn 53115 Bonn GermanyION‐GAS GmbH Konrad‐Adenauer‐Allee 11 44263 Dortmund GermanyION‐GAS GmbH Konrad‐Adenauer‐Allee 11 44263 Dortmund GermanyNees‐Institut für Biodiversität der Pflanzen, Mathematisch‐Naturwissenschaftliche Fakultät Rheinische Friedrich‐Wilhelmsuniversität Bonn 53115 Bonn GermanyAbstract Premise Daffodils (Narcissus, Amaryllidaceae) are iconic ornamentals with a complex floral biology and many fragrant species; however, little is known about floral plant volatile organic compounds (pVOCs) across the genus and additional sampling is desirable. The present study investigates whether the floral scent of 20 species of Narcissus can be characterized using gas chromatography–coupled ion mobility spectrometry (GC–IMS), with the aim of building a comparative pVOC data set for ecological and evolutionary studies. Methods We used a commercial GC–IMS equipped with an integrated in‐line enrichment system for a fast, sensitive, and automated pVOC analysis. This facilitates qualitative and (semi)‐quantitative measurements without sample preparation. Results The GC–IMS provided detailed data on floral pVOCs in Narcissus with very short sampling times and without floral enclosure. A wide range of compounds was recorded and partially identified. The retrieved pVOC patterns showed a good agreement with published data, and five “chemotypes” were characterized as characteristic combinations of floral volatiles. Discussion The GC–IMS setup can be applied to rapidly generate large amounts of pVOC data with high sensitivity and selectivity. The preliminary data on Narcissus obtained here indicate both considerable pVOC variability and a good correspondence of the pVOC patterns with infrageneric classification, supporting the hypothesis that floral scent could represent a considerable phylogenetic signal.https://doi.org/10.1002/aps3.11506benzyl acetateion mobility spectrometrylinaloolNarcissusocimeneplant volatile organic compounds |
| spellingShingle | Florian Losch Sascha Liedtke Wolfgang Vautz Maximilian Weigend Evaluation of floral volatile patterns in the genus Narcissus using gas chromatography–coupled ion mobility spectrometry Applications in Plant Sciences benzyl acetate ion mobility spectrometry linalool Narcissus ocimene plant volatile organic compounds |
| title | Evaluation of floral volatile patterns in the genus Narcissus using gas chromatography–coupled ion mobility spectrometry |
| title_full | Evaluation of floral volatile patterns in the genus Narcissus using gas chromatography–coupled ion mobility spectrometry |
| title_fullStr | Evaluation of floral volatile patterns in the genus Narcissus using gas chromatography–coupled ion mobility spectrometry |
| title_full_unstemmed | Evaluation of floral volatile patterns in the genus Narcissus using gas chromatography–coupled ion mobility spectrometry |
| title_short | Evaluation of floral volatile patterns in the genus Narcissus using gas chromatography–coupled ion mobility spectrometry |
| title_sort | evaluation of floral volatile patterns in the genus narcissus using gas chromatography coupled ion mobility spectrometry |
| topic | benzyl acetate ion mobility spectrometry linalool Narcissus ocimene plant volatile organic compounds |
| url | https://doi.org/10.1002/aps3.11506 |
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