RNA binding protein 24 regulates the translation and replication of hepatitis C virus
Abstract The secondary structures of hepatitis C virus (HCV) RNA and the cellular proteins that bind to them are important for modulating both translation and RNA replication. However, the sets of RNA-binding proteins involved in the regulation of HCV translation, replication and encapsidation remai...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2018-01-01
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| Series: | Protein & Cell |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1007/s13238-018-0507-x |
| _version_ | 1797728306127175680 |
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| author | Huang Cao Kaitao Zhao Yongxuan Yao Jing Guo Xiaoxiao Gao Qi Yang Min Guo Wandi Zhu Yun Wang Chunchen Wu Jizheng Chen Yuan Zhou Xue Hu Mengji Lu Xinwen Chen Rongjuan Pei |
| author_facet | Huang Cao Kaitao Zhao Yongxuan Yao Jing Guo Xiaoxiao Gao Qi Yang Min Guo Wandi Zhu Yun Wang Chunchen Wu Jizheng Chen Yuan Zhou Xue Hu Mengji Lu Xinwen Chen Rongjuan Pei |
| author_sort | Huang Cao |
| collection | DOAJ |
| description | Abstract The secondary structures of hepatitis C virus (HCV) RNA and the cellular proteins that bind to them are important for modulating both translation and RNA replication. However, the sets of RNA-binding proteins involved in the regulation of HCV translation, replication and encapsidation remain unknown. Here, we identified RNA binding motif protein 24 (RBM24) as a host factor participated in HCV translation and replication. Knockdown of RBM24 reduced HCV propagation in Huh7.5.1 cells. An enhanced translation and delayed RNA synthesis during the early phase of infection was observed in RBM24 silencing cells. However, both overexpression of RBM24 and recombinant human RBM24 protein suppressed HCV IRES-mediated translation. Further analysis revealed that the assembly of the 80S ribosome on the HCV IRES was interrupted by RBM24 protein through binding to the 5′-UTR. RBM24 could also interact with HCV Core and enhance the interaction of Core and 5′-UTR, which suppresses the expression of HCV. Moreover, RBM24 enhanced the interaction between the 5′- and 3′-UTRs in the HCV genome, which probably explained its requirement in HCV genome replication. Therefore, RBM24 is a novel host factor involved in HCV replication and may function at the switch from translation to replication. |
| first_indexed | 2024-03-12T11:12:07Z |
| format | Article |
| id | doaj.art-e9f98613571442ae90bb3719dace6f61 |
| institution | Directory Open Access Journal |
| issn | 1674-800X 1674-8018 |
| language | English |
| last_indexed | 2024-03-12T11:12:07Z |
| publishDate | 2018-01-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Protein & Cell |
| spelling | doaj.art-e9f98613571442ae90bb3719dace6f612023-09-02T02:46:57ZengOxford University PressProtein & Cell1674-800X1674-80182018-01-0191193094410.1007/s13238-018-0507-xRNA binding protein 24 regulates the translation and replication of hepatitis C virusHuang Cao0Kaitao Zhao1Yongxuan Yao2Jing Guo3Xiaoxiao Gao4Qi Yang5Min Guo6Wandi Zhu7Yun Wang8Chunchen Wu9Jizheng Chen10Yuan Zhou11Xue Hu12Mengji Lu13Xinwen Chen14Rongjuan Pei15State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesDepartment of Infectious Disease, University Hospital Essen, University of Duisburg-EssenState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesState Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of SciencesAbstract The secondary structures of hepatitis C virus (HCV) RNA and the cellular proteins that bind to them are important for modulating both translation and RNA replication. However, the sets of RNA-binding proteins involved in the regulation of HCV translation, replication and encapsidation remain unknown. Here, we identified RNA binding motif protein 24 (RBM24) as a host factor participated in HCV translation and replication. Knockdown of RBM24 reduced HCV propagation in Huh7.5.1 cells. An enhanced translation and delayed RNA synthesis during the early phase of infection was observed in RBM24 silencing cells. However, both overexpression of RBM24 and recombinant human RBM24 protein suppressed HCV IRES-mediated translation. Further analysis revealed that the assembly of the 80S ribosome on the HCV IRES was interrupted by RBM24 protein through binding to the 5′-UTR. RBM24 could also interact with HCV Core and enhance the interaction of Core and 5′-UTR, which suppresses the expression of HCV. Moreover, RBM24 enhanced the interaction between the 5′- and 3′-UTRs in the HCV genome, which probably explained its requirement in HCV genome replication. Therefore, RBM24 is a novel host factor involved in HCV replication and may function at the switch from translation to replication.http://link.springer.com/article/10.1007/s13238-018-0507-xRNA binding proteinRBM24hepatitis C virustranslationreplication |
| spellingShingle | Huang Cao Kaitao Zhao Yongxuan Yao Jing Guo Xiaoxiao Gao Qi Yang Min Guo Wandi Zhu Yun Wang Chunchen Wu Jizheng Chen Yuan Zhou Xue Hu Mengji Lu Xinwen Chen Rongjuan Pei RNA binding protein 24 regulates the translation and replication of hepatitis C virus Protein & Cell RNA binding protein RBM24 hepatitis C virus translation replication |
| title | RNA binding protein 24 regulates the translation and replication of hepatitis C virus |
| title_full | RNA binding protein 24 regulates the translation and replication of hepatitis C virus |
| title_fullStr | RNA binding protein 24 regulates the translation and replication of hepatitis C virus |
| title_full_unstemmed | RNA binding protein 24 regulates the translation and replication of hepatitis C virus |
| title_short | RNA binding protein 24 regulates the translation and replication of hepatitis C virus |
| title_sort | rna binding protein 24 regulates the translation and replication of hepatitis c virus |
| topic | RNA binding protein RBM24 hepatitis C virus translation replication |
| url | http://link.springer.com/article/10.1007/s13238-018-0507-x |
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