Association Between Expression of Interleukin-32 Gene and Various Helicobacter pylori Virulence Factors in Human Infected Gastric Biopsy
Helicobacter pylori (H. pylori) is a spiral bacterium that infects the human gastric mucosa. Various clinical aspects of the infection may mirror distinctive forms of cytokine expression. It has a correlation with immune cell penetration to the gastric mucosa with numerous cytokines production and g...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2020-07-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/8094 |
| Summary: | Helicobacter pylori (H. pylori) is a spiral bacterium that infects the human gastric mucosa. Various clinical aspects of the infection may mirror distinctive forms of cytokine expression. It has a correlation with immune cell penetration to the gastric mucosa with numerous cytokines production and gastric inflammation. IL-1 and IL-8 are directly contribute to H. pylori effected gastritis. IL-32 is a pro-inflammatory cytokine categorized by the training of Immune cells activation which have a vital role in human immunity. H. pylori virulence and danger factors which are critical in gastritis such as the outer inflammatory protein (OipA) and the cytotoxin associated gene A (cagA). We aimed to study the IL-32 mRNA expression in H. pylori-positive and negative patients as well as its relation with bacterial cagA, oipA, and severity of gastritis. Endoscopic biopsies were taken from the antrum of 60 H. pylori-infected patients and 62 uninfected individuals. Mucosal IL-32 mRNA expression was assessed by real-time PCR. With PCR the H. pylori virulence factors were evaluated. Showed that the mRNA expression of IL-32 levels were significantly lower in biopsies of H. pylori-uninfected patients compared to positive individual (p = 0.01). A straight communication between virulence factor oipA, cagA and heightening in IL-32 mRNA expression (p <0.001) was observed. Furthermore. IL-32 mRNA expression levels were approximately equal in both chronic and active gastritis (p = 0.1). IL-32 may have critical role in different situation like inflammation and severity of inflammatory changes in the gastric mucosa. |
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| ISSN: | 0044-6025 1735-9694 |