OsCBL1 modulates rice nitrogen use efficiency via negative regulation of OsNRT2.2 by OsCCA1
Abstract Background For cereal crop breeding, it is meaningful to improve utilization efficiency (NUE) under low nitrogen (LN) levels while maintaining crop yield. OsCBL1-knockdown (OsCBL1-KD) plants exhibited increased nitrogen accumulation and NUE in the field of low N level. Results OsCBL1-knockd...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04520-4 |
| _version_ | 1827635059672219648 |
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| author | Zhao Hu Yutan Guo Suping Ying Yunting Tang Jiawei Niu Ting Wang Ruifeng Huang Hongwei Xie Wenya Wang Xiaojue Peng |
| author_facet | Zhao Hu Yutan Guo Suping Ying Yunting Tang Jiawei Niu Ting Wang Ruifeng Huang Hongwei Xie Wenya Wang Xiaojue Peng |
| author_sort | Zhao Hu |
| collection | DOAJ |
| description | Abstract Background For cereal crop breeding, it is meaningful to improve utilization efficiency (NUE) under low nitrogen (LN) levels while maintaining crop yield. OsCBL1-knockdown (OsCBL1-KD) plants exhibited increased nitrogen accumulation and NUE in the field of low N level. Results OsCBL1-knockdown (OsCBL1-KD) in rice increased the expression of a nitrate transporter gene OsNRT2.2. In addition, the expression of OsNRT2.2, was suppressed by OsCCA1, a negative regulator, which could directly bind to the MYB-binding elements (EE) in the region of OsNRT2.2 promoter. The OsCCA1 expression was found to be down-regulated in OsCBL1-KD plants. At the low Nitrogen (N) level field, the OsCBL1-KD plants exhibited a substantial accumulation of content and higher NUE, and their actual biomass remained approximately as the same as that of the wild type. Conclusion These results indicated that down-regulation of OsCBL1 expression could upregulate the expression of OsNRT2.2 by suppressing the expression of OsCCA1and then increasing the NUE of OsCBL1-KD plants under low nitrogen availability. |
| first_indexed | 2024-03-09T15:21:56Z |
| format | Article |
| id | doaj.art-e9fcfdb82c1d41f59a79229572260601 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-09T15:21:56Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-e9fcfdb82c1d41f59a792295722606012023-11-26T12:46:52ZengBMCBMC Plant Biology1471-22292023-10-0123111410.1186/s12870-023-04520-4OsCBL1 modulates rice nitrogen use efficiency via negative regulation of OsNRT2.2 by OsCCA1Zhao Hu0Yutan Guo1Suping Ying2Yunting Tang3Jiawei Niu4Ting Wang5Ruifeng Huang6Hongwei Xie7Wenya Wang8Xiaojue Peng9Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang UniversityKey Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang UniversityKey Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang UniversityKey Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang UniversityKey Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang UniversityDepartment of Chemistry, University of KentuckyKey Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang UniversityJiangxi Super-rice Research and Development center, National Engineering Laboratory for RiceMsc Applied Genomics, Imperial College LondonKey Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang UniversityAbstract Background For cereal crop breeding, it is meaningful to improve utilization efficiency (NUE) under low nitrogen (LN) levels while maintaining crop yield. OsCBL1-knockdown (OsCBL1-KD) plants exhibited increased nitrogen accumulation and NUE in the field of low N level. Results OsCBL1-knockdown (OsCBL1-KD) in rice increased the expression of a nitrate transporter gene OsNRT2.2. In addition, the expression of OsNRT2.2, was suppressed by OsCCA1, a negative regulator, which could directly bind to the MYB-binding elements (EE) in the region of OsNRT2.2 promoter. The OsCCA1 expression was found to be down-regulated in OsCBL1-KD plants. At the low Nitrogen (N) level field, the OsCBL1-KD plants exhibited a substantial accumulation of content and higher NUE, and their actual biomass remained approximately as the same as that of the wild type. Conclusion These results indicated that down-regulation of OsCBL1 expression could upregulate the expression of OsNRT2.2 by suppressing the expression of OsCCA1and then increasing the NUE of OsCBL1-KD plants under low nitrogen availability.https://doi.org/10.1186/s12870-023-04520-4Oryza stativaOsCBL1OsCCA1OsNRT2.2NUE |
| spellingShingle | Zhao Hu Yutan Guo Suping Ying Yunting Tang Jiawei Niu Ting Wang Ruifeng Huang Hongwei Xie Wenya Wang Xiaojue Peng OsCBL1 modulates rice nitrogen use efficiency via negative regulation of OsNRT2.2 by OsCCA1 BMC Plant Biology Oryza stativa OsCBL1 OsCCA1 OsNRT2.2 NUE |
| title | OsCBL1 modulates rice nitrogen use efficiency via negative regulation of OsNRT2.2 by OsCCA1 |
| title_full | OsCBL1 modulates rice nitrogen use efficiency via negative regulation of OsNRT2.2 by OsCCA1 |
| title_fullStr | OsCBL1 modulates rice nitrogen use efficiency via negative regulation of OsNRT2.2 by OsCCA1 |
| title_full_unstemmed | OsCBL1 modulates rice nitrogen use efficiency via negative regulation of OsNRT2.2 by OsCCA1 |
| title_short | OsCBL1 modulates rice nitrogen use efficiency via negative regulation of OsNRT2.2 by OsCCA1 |
| title_sort | oscbl1 modulates rice nitrogen use efficiency via negative regulation of osnrt2 2 by oscca1 |
| topic | Oryza stativa OsCBL1 OsCCA1 OsNRT2.2 NUE |
| url | https://doi.org/10.1186/s12870-023-04520-4 |
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