Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii
This work was carried out to select and to evaluate efficacy of Trichoderma isolates to control sclerotium wilt (Sclerotium rolfsii) of common-bean (Phaseolus vulgaris). For the experiments were used two isolates of S. rolfsii UB 193 and UB 228. Sixty-five Trichoderma spp. isolates were tested and t...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Uberlândia
2016-04-01
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| Series: | Bioscience Journal |
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| Online Access: | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/32732 |
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| author | Klênia Rodrigues Pacheco Bernardo Souza Mello Viscardi Thais Melissa Macedo de Vasconcelos Geisianny Augusta Monteiro Moreira Helson Mario Martins do Vale Luiz Eduardo Bassay Blum |
| author_facet | Klênia Rodrigues Pacheco Bernardo Souza Mello Viscardi Thais Melissa Macedo de Vasconcelos Geisianny Augusta Monteiro Moreira Helson Mario Martins do Vale Luiz Eduardo Bassay Blum |
| author_sort | Klênia Rodrigues Pacheco |
| collection | DOAJ |
| description | This work was carried out to select and to evaluate efficacy of Trichoderma isolates to control sclerotium wilt (Sclerotium rolfsii) of common-bean (Phaseolus vulgaris). For the experiments were used two isolates of S. rolfsii UB 193 and UB 228. Sixty-five Trichoderma spp. isolates were tested and the following ones were selected in vitro for the in vivo tests: 5, 11, 12, 15, 102, 103, 127, 136, 137, 1525 (T. longibrachiatum), 1637 (T. reesei), 1642, 1643 (T. harzianum), 1649 (T. harzianum), 1700 (T. asperellum) and EST 5. The most promising isolates were identified by Sequencing of the internal transcribed spacer regions ITS1, ITS2, and the 5.8s rRNA genomic region, using the ITS5 and ITS4 primers and compared with sequences in the National Center for Biotechnology Information (NCBI) database. These selected isolates 1649 (T. harzianum), 1525 (T. longibrachiatum) and 1637 (T. reesei) were tested for evaluation of sclerotial germination inhibition under laboratory conditions, and to evaluate the effects of these on disease of bean plants under greenhouse conditions. The Trichoderma isolates 1649, 1525 and 1637 were more efficient in reducing sclerotial germination. In addition to 1649, 1525 and 1637, the isolates 5, 12, 102 and 1525 (T. longibrachiatum) significantly reduced de amount of diseased bean plants under greenhouse conditions. |
| first_indexed | 2024-12-19T21:48:35Z |
| format | Article |
| id | doaj.art-e9fdb02eea2b4aabb60da72af9bb5c77 |
| institution | Directory Open Access Journal |
| issn | 1981-3163 |
| language | English |
| last_indexed | 2024-12-19T21:48:35Z |
| publishDate | 2016-04-01 |
| publisher | Universidade Federal de Uberlândia |
| record_format | Article |
| series | Bioscience Journal |
| spelling | doaj.art-e9fdb02eea2b4aabb60da72af9bb5c772022-12-21T20:04:28ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632016-04-0132210.14393/BJ-v32n2a2016-3273232732Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsiiKlênia Rodrigues PachecoBernardo Souza Mello ViscardiThais Melissa Macedo de VasconcelosGeisianny Augusta Monteiro MoreiraHelson Mario Martins do ValeLuiz Eduardo Bassay BlumThis work was carried out to select and to evaluate efficacy of Trichoderma isolates to control sclerotium wilt (Sclerotium rolfsii) of common-bean (Phaseolus vulgaris). For the experiments were used two isolates of S. rolfsii UB 193 and UB 228. Sixty-five Trichoderma spp. isolates were tested and the following ones were selected in vitro for the in vivo tests: 5, 11, 12, 15, 102, 103, 127, 136, 137, 1525 (T. longibrachiatum), 1637 (T. reesei), 1642, 1643 (T. harzianum), 1649 (T. harzianum), 1700 (T. asperellum) and EST 5. The most promising isolates were identified by Sequencing of the internal transcribed spacer regions ITS1, ITS2, and the 5.8s rRNA genomic region, using the ITS5 and ITS4 primers and compared with sequences in the National Center for Biotechnology Information (NCBI) database. These selected isolates 1649 (T. harzianum), 1525 (T. longibrachiatum) and 1637 (T. reesei) were tested for evaluation of sclerotial germination inhibition under laboratory conditions, and to evaluate the effects of these on disease of bean plants under greenhouse conditions. The Trichoderma isolates 1649, 1525 and 1637 were more efficient in reducing sclerotial germination. In addition to 1649, 1525 and 1637, the isolates 5, 12, 102 and 1525 (T. longibrachiatum) significantly reduced de amount of diseased bean plants under greenhouse conditions.http://www.seer.ufu.br/index.php/biosciencejournal/article/view/32732aethelia rolfsiiphaseolus vulgarisbiocontroltrichoderma spp. |
| spellingShingle | Klênia Rodrigues Pacheco Bernardo Souza Mello Viscardi Thais Melissa Macedo de Vasconcelos Geisianny Augusta Monteiro Moreira Helson Mario Martins do Vale Luiz Eduardo Bassay Blum Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii Bioscience Journal aethelia rolfsii phaseolus vulgaris biocontrol trichoderma spp. |
| title | Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii |
| title_full | Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii |
| title_fullStr | Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii |
| title_full_unstemmed | Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii |
| title_short | Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii |
| title_sort | efficacy of trichoderma asperellum t harzianum t longibrachiatum and t reesei against sclerotium rolfsii |
| topic | aethelia rolfsii phaseolus vulgaris biocontrol trichoderma spp. |
| url | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/32732 |
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