Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense
Cysteine-rich receptor-like kinases (CRKs) are a large subfamily of plant receptor-like kinases that play a critical role in disease resistance in plants. However, knowledge about the CRK gene family in cotton and its function against Verticillium wilt (VW), a destructive disease caused by Verticill...
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2018.01266/full |
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| author | Ting-Gang Li Dan-Dan Zhang Lei Zhou Zhi-Qiang Kong Adamu S. Hussaini Dan Wang Jun-Jiao Li Dylan P. G. Short Dylan P. G. Short Nikhilesh Dhar Nikhilesh Dhar Steven J. Klosterman Bao-Li Wang Chun-Mei Yin Krishna V. Subbarao Krishna V. Subbarao Jie-Yin Chen Xiao-Feng Dai |
| author_facet | Ting-Gang Li Dan-Dan Zhang Lei Zhou Zhi-Qiang Kong Adamu S. Hussaini Dan Wang Jun-Jiao Li Dylan P. G. Short Dylan P. G. Short Nikhilesh Dhar Nikhilesh Dhar Steven J. Klosterman Bao-Li Wang Chun-Mei Yin Krishna V. Subbarao Krishna V. Subbarao Jie-Yin Chen Xiao-Feng Dai |
| author_sort | Ting-Gang Li |
| collection | DOAJ |
| description | Cysteine-rich receptor-like kinases (CRKs) are a large subfamily of plant receptor-like kinases that play a critical role in disease resistance in plants. However, knowledge about the CRK gene family in cotton and its function against Verticillium wilt (VW), a destructive disease caused by Verticillium dahliae that significantly reduces cotton yields is lacking. In this study, we identified a total of 30 typical CRKs in a Gossypium barbadense genome (GbCRKs). Eleven of these (>30%) are located on the A06 and D06 chromosomes, and 18 consisted of 9 paralogous pairs encoded in the A and D subgenomes. Phylogenetic analysis showed that the GbCRKs could be classified into four broad groups, the expansion of which has probably been driven by tandem duplication. Gene expression profiling of the GbCRKs in resistant and susceptible cotton cultivars revealed that a phylogenetic cluster of nine of the GbCRK genes were up-regulated in response to V. dahliae infection. Virus-induced gene silencing of each of these nine GbCRKs independently revealed that the silencing of GbCRK18 was sufficient to compromise VW resistance in G. barbadense. GbCRK18 expression could be induced by V. dahliae infection or jasmonic acid, and displayed plasma membrane localization. Therefore, our expression analyses indicated that the CRK gene family is differentially regulated in response to Verticillium infection, while gene silencing experiments revealed that GbCRK18 in particular confers VW resistance in G. barbadense. |
| first_indexed | 2024-12-10T18:33:06Z |
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| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T18:33:06Z |
| publishDate | 2018-09-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-e9ff6715bf8a4b0082d26f77fef5b0fa2022-12-22T01:37:53ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-09-01910.3389/fpls.2018.01266376408Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadenseTing-Gang Li0Dan-Dan Zhang1Lei Zhou2Zhi-Qiang Kong3Adamu S. Hussaini4Dan Wang5Jun-Jiao Li6Dylan P. G. Short7Dylan P. G. Short8Nikhilesh Dhar9Nikhilesh Dhar10Steven J. Klosterman11Bao-Li Wang12Chun-Mei Yin13Krishna V. Subbarao14Krishna V. Subbarao15Jie-Yin Chen16Xiao-Feng Dai17Laboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Pathology, University of California, Davis, Davis, CA, United StatesU.S. Agricultural Research Station, Salinas, CA, United StatesDepartment of Plant Pathology, University of California, Davis, Davis, CA, United StatesU.S. Agricultural Research Station, Salinas, CA, United StatesCrop Improvement and Protection Research Unit, United States Department of Agriculture, Agricultural Research Service, Salinas, CA, United StatesLaboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Plant Pathology, University of California, Davis, Davis, CA, United StatesU.S. Agricultural Research Station, Salinas, CA, United StatesLaboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaCysteine-rich receptor-like kinases (CRKs) are a large subfamily of plant receptor-like kinases that play a critical role in disease resistance in plants. However, knowledge about the CRK gene family in cotton and its function against Verticillium wilt (VW), a destructive disease caused by Verticillium dahliae that significantly reduces cotton yields is lacking. In this study, we identified a total of 30 typical CRKs in a Gossypium barbadense genome (GbCRKs). Eleven of these (>30%) are located on the A06 and D06 chromosomes, and 18 consisted of 9 paralogous pairs encoded in the A and D subgenomes. Phylogenetic analysis showed that the GbCRKs could be classified into four broad groups, the expansion of which has probably been driven by tandem duplication. Gene expression profiling of the GbCRKs in resistant and susceptible cotton cultivars revealed that a phylogenetic cluster of nine of the GbCRK genes were up-regulated in response to V. dahliae infection. Virus-induced gene silencing of each of these nine GbCRKs independently revealed that the silencing of GbCRK18 was sufficient to compromise VW resistance in G. barbadense. GbCRK18 expression could be induced by V. dahliae infection or jasmonic acid, and displayed plasma membrane localization. Therefore, our expression analyses indicated that the CRK gene family is differentially regulated in response to Verticillium infection, while gene silencing experiments revealed that GbCRK18 in particular confers VW resistance in G. barbadense.https://www.frontiersin.org/article/10.3389/fpls.2018.01266/fullcottonVerticillium wiltcysteine-rich receptor-like kinases (CRKs)expression profilingdefense response |
| spellingShingle | Ting-Gang Li Dan-Dan Zhang Lei Zhou Zhi-Qiang Kong Adamu S. Hussaini Dan Wang Jun-Jiao Li Dylan P. G. Short Dylan P. G. Short Nikhilesh Dhar Nikhilesh Dhar Steven J. Klosterman Bao-Li Wang Chun-Mei Yin Krishna V. Subbarao Krishna V. Subbarao Jie-Yin Chen Xiao-Feng Dai Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense Frontiers in Plant Science cotton Verticillium wilt cysteine-rich receptor-like kinases (CRKs) expression profiling defense response |
| title | Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense |
| title_full | Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense |
| title_fullStr | Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense |
| title_full_unstemmed | Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense |
| title_short | Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense |
| title_sort | genome wide identification and functional analyses of the crk gene family in cotton reveals gbcrk18 confers verticillium wilt resistance in gossypium barbadense |
| topic | cotton Verticillium wilt cysteine-rich receptor-like kinases (CRKs) expression profiling defense response |
| url | https://www.frontiersin.org/article/10.3389/fpls.2018.01266/full |
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