Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression
Microbiome management is a promising way to suppress verticillium wilt, a severe disease in <i>Brassica</i> caused by <i>Verticillium longisporum</i>. In order to improve current biocontrol strategies, we compared bacterial <i>Verticillium</i> antagonists in diffe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/7/866 |
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| author | Daria Rybakova Mariann Wikström Fia Birch-Jensen Joeke Postma Ralf Udo Ehlers Maria Schmuck René Kollmann Jürgen Köhl Gabriele Berg |
| author_facet | Daria Rybakova Mariann Wikström Fia Birch-Jensen Joeke Postma Ralf Udo Ehlers Maria Schmuck René Kollmann Jürgen Köhl Gabriele Berg |
| author_sort | Daria Rybakova |
| collection | DOAJ |
| description | Microbiome management is a promising way to suppress verticillium wilt, a severe disease in <i>Brassica</i> caused by <i>Verticillium longisporum</i>. In order to improve current biocontrol strategies, we compared bacterial <i>Verticillium</i> antagonists in different assays using a hierarchical selection and evaluation scheme, and we integrated outcomes of our previous studies. The result was strongly dependent on the assessment method chosen (in vitro, in vivo, in situ), on the growth conditions of the plants and their genotype. The most promising biocontrol candidate identified was a <i>Brassica</i> endophyte <i>Serratia plymuthica</i> F20. Positive results were confirmed in field trials and by microscopically visualizing the three-way interaction. Applying antagonists in seed treatment contributes to an exceptionally low ecological footprint, supporting efficient economic and ecological solutions to controlling verticillium wilt. Indigenous microbiome, especially soil and seed microbiome, has been identified as key to understanding disease outbreaks and suppression. We suggest that verticillium wilt is a microbiome-driven disease caused by a reduction in microbial diversity within seeds and in the soil surrounding them. We strongly recommend integrating microbiome data in the development of new biocontrol and breeding strategies and combining both strategies with the aim of designing healthy microbiomes, thus making plants more resilient toward soil-borne pathogens. |
| first_indexed | 2024-03-10T18:37:02Z |
| format | Article |
| id | doaj.art-ea0530a3ebd748138d9189cec49de5c6 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T18:37:02Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-ea0530a3ebd748138d9189cec49de5c62023-11-20T06:13:00ZengMDPI AGPlants2223-77472020-07-019786610.3390/plants9070866Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient SuppressionDaria Rybakova0Mariann Wikström1Fia Birch-Jensen2Joeke Postma3Ralf Udo Ehlers4Maria Schmuck5René Kollmann6Jürgen Köhl7Gabriele Berg8Graz University of Technology, Environmental Biotechnology, Petersgasse 12, 8010 Graz, AustriaAgro Plantarum AB, Kärrarpsvägen 410, S-265 90 Åstorp, SwedenAgro Plantarum AB, Kärrarpsvägen 410, S-265 90 Åstorp, SwedenWageningen University & Research, 6708 PB Wageningen, NetherlandsE-nema GmbH, Klausdorfer Str. 28–36, 24223 Schwentinental, GermanyGraz University of Technology, Environmental Biotechnology, Petersgasse 12, 8010 Graz, AustriaStrateco OG, Ruckerlberggasse 13, 8010 Graz, AustriaWageningen University & Research, 6708 PB Wageningen, NetherlandsGraz University of Technology, Environmental Biotechnology, Petersgasse 12, 8010 Graz, AustriaMicrobiome management is a promising way to suppress verticillium wilt, a severe disease in <i>Brassica</i> caused by <i>Verticillium longisporum</i>. In order to improve current biocontrol strategies, we compared bacterial <i>Verticillium</i> antagonists in different assays using a hierarchical selection and evaluation scheme, and we integrated outcomes of our previous studies. The result was strongly dependent on the assessment method chosen (in vitro, in vivo, in situ), on the growth conditions of the plants and their genotype. The most promising biocontrol candidate identified was a <i>Brassica</i> endophyte <i>Serratia plymuthica</i> F20. Positive results were confirmed in field trials and by microscopically visualizing the three-way interaction. Applying antagonists in seed treatment contributes to an exceptionally low ecological footprint, supporting efficient economic and ecological solutions to controlling verticillium wilt. Indigenous microbiome, especially soil and seed microbiome, has been identified as key to understanding disease outbreaks and suppression. We suggest that verticillium wilt is a microbiome-driven disease caused by a reduction in microbial diversity within seeds and in the soil surrounding them. We strongly recommend integrating microbiome data in the development of new biocontrol and breeding strategies and combining both strategies with the aim of designing healthy microbiomes, thus making plants more resilient toward soil-borne pathogens.https://www.mdpi.com/2223-7747/9/7/866oilseed rapecanolamicrobiomebacterial communitybiocontrolendophytes |
| spellingShingle | Daria Rybakova Mariann Wikström Fia Birch-Jensen Joeke Postma Ralf Udo Ehlers Maria Schmuck René Kollmann Jürgen Köhl Gabriele Berg Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression Plants oilseed rape canola microbiome bacterial community biocontrol endophytes |
| title | Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression |
| title_full | Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression |
| title_fullStr | Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression |
| title_full_unstemmed | Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression |
| title_short | Verticillium Wilt in Oilseed Rape—the Microbiome is Crucial for Disease Outbreaks as Well as for Efficient Suppression |
| title_sort | verticillium wilt in oilseed rape the microbiome is crucial for disease outbreaks as well as for efficient suppression |
| topic | oilseed rape canola microbiome bacterial community biocontrol endophytes |
| url | https://www.mdpi.com/2223-7747/9/7/866 |
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