Biofilm Formation of <i>Staphylococcus aureus</i> from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance
This study aimed to compare the biofilm formation ability of <i>Staphylococcus aureus</i> isolated from a wide range of animals and study the association between biofilm formation and antimicrobial resistance and genetic lineages. A total of 214 <i>S. aureus</i> strains isola...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/6/772 |
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| author | Vanessa Silva Elisete Correia José Eduardo Pereira Camino González-Machado Rosa Capita Carlos Alonso-Calleja Gilberto Igrejas Patrícia Poeta |
| author_facet | Vanessa Silva Elisete Correia José Eduardo Pereira Camino González-Machado Rosa Capita Carlos Alonso-Calleja Gilberto Igrejas Patrícia Poeta |
| author_sort | Vanessa Silva |
| collection | DOAJ |
| description | This study aimed to compare the biofilm formation ability of <i>Staphylococcus aureus</i> isolated from a wide range of animals and study the association between biofilm formation and antimicrobial resistance and genetic lineages. A total of 214 <i>S. aureus</i> strains isolated from pets, livestock, and wild animals were evaluated regarding their ability to form biofilms by the microtiter biofilm assay and their structure via confocal scanning laser microscopy. Statistical analysis was used to find an association between biofilm formation and antimicrobial resistance, multidrug resistance, sequence types (STs), <i>spa</i> and <i>agr</i>-types of the isolates. The antimicrobial susceptibility of 24 h-old biofilms was assessed against minimum inhibitory concentrations (MIC) and 10× MIC of amikacin and tetracycline, and the biomass reduction was measured. The metabolic activity of biofilms after antimicrobial treatment was evaluated by the XTT assay. All isolates were had the ability to form biofilms. Yet, significant differences in biofilm biomass production were detected among animal species. Multidrug resistance had a positive association with biofilm formation as well as methicillin-resistance. Significant differences were also detected among the clonal lineages of the isolates. Both tetracycline and amikacin were able to significantly reduce the biofilm mass. However, none of the antimicrobials were able to eradicate the biofilm at the maximum concentration used. Our results provide important information on the biofilm-forming capacity of animal-adapted <i>S. aureus</i> isolates, which may have potential implications for the development of new biofilm-targeted therapeutics. |
| first_indexed | 2024-03-10T00:35:54Z |
| format | Article |
| id | doaj.art-ea0900a0d5b443a48ecd11f76d688ca7 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T00:35:54Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-ea0900a0d5b443a48ecd11f76d688ca72023-11-23T15:17:01ZengMDPI AGAntibiotics2079-63822022-06-0111677210.3390/antibiotics11060772Biofilm Formation of <i>Staphylococcus aureus</i> from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial ResistanceVanessa Silva0Elisete Correia1José Eduardo Pereira2Camino González-Machado3Rosa Capita4Carlos Alonso-Calleja5Gilberto Igrejas6Patrícia Poeta7Microbiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalCenter for Computational and Stochastic Mathematics (CEMAT), Department of Mathematics, University of Trás-os-Montes and Alto Douro (UTAD), 5001-801 Vila Real, PortugalCECAV—Veterinary and Animal Research Centre, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalDepartment of Food Hygiene and Technology, Veterinary Faculty, University of León, E-24071 León, SpainDepartment of Food Hygiene and Technology, Veterinary Faculty, University of León, E-24071 León, SpainDepartment of Food Hygiene and Technology, Veterinary Faculty, University of León, E-24071 León, SpainDepartment of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalMicrobiology and Antibiotic Resistance Team (MicroART), Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, PortugalThis study aimed to compare the biofilm formation ability of <i>Staphylococcus aureus</i> isolated from a wide range of animals and study the association between biofilm formation and antimicrobial resistance and genetic lineages. A total of 214 <i>S. aureus</i> strains isolated from pets, livestock, and wild animals were evaluated regarding their ability to form biofilms by the microtiter biofilm assay and their structure via confocal scanning laser microscopy. Statistical analysis was used to find an association between biofilm formation and antimicrobial resistance, multidrug resistance, sequence types (STs), <i>spa</i> and <i>agr</i>-types of the isolates. The antimicrobial susceptibility of 24 h-old biofilms was assessed against minimum inhibitory concentrations (MIC) and 10× MIC of amikacin and tetracycline, and the biomass reduction was measured. The metabolic activity of biofilms after antimicrobial treatment was evaluated by the XTT assay. All isolates were had the ability to form biofilms. Yet, significant differences in biofilm biomass production were detected among animal species. Multidrug resistance had a positive association with biofilm formation as well as methicillin-resistance. Significant differences were also detected among the clonal lineages of the isolates. Both tetracycline and amikacin were able to significantly reduce the biofilm mass. However, none of the antimicrobials were able to eradicate the biofilm at the maximum concentration used. Our results provide important information on the biofilm-forming capacity of animal-adapted <i>S. aureus</i> isolates, which may have potential implications for the development of new biofilm-targeted therapeutics.https://www.mdpi.com/2079-6382/11/6/772<i>S. aureus</i>MRSAanimalsbiofilmsantimicrobial resistancegenetic linages |
| spellingShingle | Vanessa Silva Elisete Correia José Eduardo Pereira Camino González-Machado Rosa Capita Carlos Alonso-Calleja Gilberto Igrejas Patrícia Poeta Biofilm Formation of <i>Staphylococcus aureus</i> from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance Antibiotics <i>S. aureus</i> MRSA animals biofilms antimicrobial resistance genetic linages |
| title | Biofilm Formation of <i>Staphylococcus aureus</i> from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance |
| title_full | Biofilm Formation of <i>Staphylococcus aureus</i> from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance |
| title_fullStr | Biofilm Formation of <i>Staphylococcus aureus</i> from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance |
| title_full_unstemmed | Biofilm Formation of <i>Staphylococcus aureus</i> from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance |
| title_short | Biofilm Formation of <i>Staphylococcus aureus</i> from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance |
| title_sort | biofilm formation of i staphylococcus aureus i from pets livestock and wild animals relationship with clonal lineages and antimicrobial resistance |
| topic | <i>S. aureus</i> MRSA animals biofilms antimicrobial resistance genetic linages |
| url | https://www.mdpi.com/2079-6382/11/6/772 |
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