Abnormal Blood Bacteriome, Gut Dysbiosis, and Progression to Severe Dengue Disease
Despite a well-known association between gut barrier defect (leaky gut) and several diseases, data on translocation of pathogen molecules, including bacterial DNA (blood bacteriome), lipopolysaccharide (LPS), and serum (1→3)-β-D-glucan (BG), from the gut to the blood circulation (gut translocation)...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.890817/full |
| _version_ | 1811337422905016320 |
|---|---|
| author | Wiwat Chancharoenthana Wiwat Chancharoenthana Supitcha Kamolratanakul Supitcha Kamolratanakul Wassawon Ariyanon Wassawon Ariyanon Vipa Thanachartwet Weerapong Phumratanaprapin Polrat Wilairatana Asada Leelahavanichkul Asada Leelahavanichkul |
| author_facet | Wiwat Chancharoenthana Wiwat Chancharoenthana Supitcha Kamolratanakul Supitcha Kamolratanakul Wassawon Ariyanon Wassawon Ariyanon Vipa Thanachartwet Weerapong Phumratanaprapin Polrat Wilairatana Asada Leelahavanichkul Asada Leelahavanichkul |
| author_sort | Wiwat Chancharoenthana |
| collection | DOAJ |
| description | Despite a well-known association between gut barrier defect (leaky gut) and several diseases, data on translocation of pathogen molecules, including bacterial DNA (blood bacteriome), lipopolysaccharide (LPS), and serum (1→3)-β-D-glucan (BG), from the gut to the blood circulation (gut translocation) in dengue are still less studied. Perhaps, dengue infection might induce gut translocation of several pathogenic molecules that affect the disease severity. At the enrollment, there were 31 dengue cases in febrile and critical phases at 4.1 ± 0.3 days and 6.4 ± 1.1 days of illness, respectively, with the leaky gut as indicated by positive lactulose-to-mannitol excretion ratio. With blood bacteriome, the patients with critical phase (more severe dengue; n = 23) demonstrated more predominant abundance in Bacteroidetes and Escherichia spp. with the lower Bifidobacteria when compared with the healthy control (n = 5). Meanwhile, most of the blood bacteriome results in dengue with febrile stage (n = 8) were comparable to the control, except for the lower Bifidobacteria in dengue cases. Additionally, endotoxemia at the enrollment was demonstrated in five (62.5%) and 19 (82.6%) patients with febrile and critical phases, respectively, while serum BG was detectable in two (25%) and 20 (87%) patients with febrile and critical phases, respectively. There were higher peripheral blood non-classical monocytes and natural killer cells (NK cells) at the enrollment in patients with febrile phage than in the cases with critical stage. Then, non-classical monocytes (CD14-CD16+) and NK cells (CD56+CD16-) increased at 4 and 7 days of illness in the cases with critical and febrile stages, respectively, the elevation of LPS and/or BG in serum on day 7 was also associated with the increase in monocytes, NK cells, and cytotoxic T cells. In summary, enhanced Proteobacteria (pathogenic bacteria from blood bacteriomes) along with increased endotoxemia and serum BG (leaky gut syndrome) might be collaborated with the impaired microbial control (lower non-classical monocytes and NK cells) in the critical cases and causing more severe disease of dengue infection. |
| first_indexed | 2024-04-13T17:54:41Z |
| format | Article |
| id | doaj.art-ea0b4092f9574acd83f92ab2defdf851 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-13T17:54:41Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-ea0b4092f9574acd83f92ab2defdf8512022-12-22T02:36:33ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-06-011210.3389/fcimb.2022.890817890817Abnormal Blood Bacteriome, Gut Dysbiosis, and Progression to Severe Dengue DiseaseWiwat Chancharoenthana0Wiwat Chancharoenthana1Supitcha Kamolratanakul2Supitcha Kamolratanakul3Wassawon Ariyanon4Wassawon Ariyanon5Vipa Thanachartwet6Weerapong Phumratanaprapin7Polrat Wilairatana8Asada Leelahavanichkul9Asada Leelahavanichkul10Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandTropical Immunology and Translational Research Unit, Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandDepartment of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandTropical Immunology and Translational Research Unit, Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandCardiometabolic Centre, Department of Medicine, Bangkok Nursing Hospital, Bangkok, ThailandDepartment of Medicine, Banphaeo General Hospital, Samutsakhon, ThailandDepartment of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandDepartment of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandDepartment of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, ThailandImmunology Unit, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, ThailandCenter of Excellence on Translational Research in Inflammation and Immunology (CETRII), Department of Microbiology, Chulalongkorn University, Bangkok, ThailandDespite a well-known association between gut barrier defect (leaky gut) and several diseases, data on translocation of pathogen molecules, including bacterial DNA (blood bacteriome), lipopolysaccharide (LPS), and serum (1→3)-β-D-glucan (BG), from the gut to the blood circulation (gut translocation) in dengue are still less studied. Perhaps, dengue infection might induce gut translocation of several pathogenic molecules that affect the disease severity. At the enrollment, there were 31 dengue cases in febrile and critical phases at 4.1 ± 0.3 days and 6.4 ± 1.1 days of illness, respectively, with the leaky gut as indicated by positive lactulose-to-mannitol excretion ratio. With blood bacteriome, the patients with critical phase (more severe dengue; n = 23) demonstrated more predominant abundance in Bacteroidetes and Escherichia spp. with the lower Bifidobacteria when compared with the healthy control (n = 5). Meanwhile, most of the blood bacteriome results in dengue with febrile stage (n = 8) were comparable to the control, except for the lower Bifidobacteria in dengue cases. Additionally, endotoxemia at the enrollment was demonstrated in five (62.5%) and 19 (82.6%) patients with febrile and critical phases, respectively, while serum BG was detectable in two (25%) and 20 (87%) patients with febrile and critical phases, respectively. There were higher peripheral blood non-classical monocytes and natural killer cells (NK cells) at the enrollment in patients with febrile phage than in the cases with critical stage. Then, non-classical monocytes (CD14-CD16+) and NK cells (CD56+CD16-) increased at 4 and 7 days of illness in the cases with critical and febrile stages, respectively, the elevation of LPS and/or BG in serum on day 7 was also associated with the increase in monocytes, NK cells, and cytotoxic T cells. In summary, enhanced Proteobacteria (pathogenic bacteria from blood bacteriomes) along with increased endotoxemia and serum BG (leaky gut syndrome) might be collaborated with the impaired microbial control (lower non-classical monocytes and NK cells) in the critical cases and causing more severe disease of dengue infection.https://www.frontiersin.org/articles/10.3389/fcimb.2022.890817/fulldengue infectionimmunityleaky gut syndromelipopolysaccaridebeta-D-glucanmicrobiome & dysbiosis |
| spellingShingle | Wiwat Chancharoenthana Wiwat Chancharoenthana Supitcha Kamolratanakul Supitcha Kamolratanakul Wassawon Ariyanon Wassawon Ariyanon Vipa Thanachartwet Weerapong Phumratanaprapin Polrat Wilairatana Asada Leelahavanichkul Asada Leelahavanichkul Abnormal Blood Bacteriome, Gut Dysbiosis, and Progression to Severe Dengue Disease Frontiers in Cellular and Infection Microbiology dengue infection immunity leaky gut syndrome lipopolysaccaride beta-D-glucan microbiome & dysbiosis |
| title | Abnormal Blood Bacteriome, Gut Dysbiosis, and Progression to Severe Dengue Disease |
| title_full | Abnormal Blood Bacteriome, Gut Dysbiosis, and Progression to Severe Dengue Disease |
| title_fullStr | Abnormal Blood Bacteriome, Gut Dysbiosis, and Progression to Severe Dengue Disease |
| title_full_unstemmed | Abnormal Blood Bacteriome, Gut Dysbiosis, and Progression to Severe Dengue Disease |
| title_short | Abnormal Blood Bacteriome, Gut Dysbiosis, and Progression to Severe Dengue Disease |
| title_sort | abnormal blood bacteriome gut dysbiosis and progression to severe dengue disease |
| topic | dengue infection immunity leaky gut syndrome lipopolysaccaride beta-D-glucan microbiome & dysbiosis |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.890817/full |
| work_keys_str_mv | AT wiwatchancharoenthana abnormalbloodbacteriomegutdysbiosisandprogressiontoseveredenguedisease AT wiwatchancharoenthana abnormalbloodbacteriomegutdysbiosisandprogressiontoseveredenguedisease AT supitchakamolratanakul abnormalbloodbacteriomegutdysbiosisandprogressiontoseveredenguedisease AT supitchakamolratanakul abnormalbloodbacteriomegutdysbiosisandprogressiontoseveredenguedisease AT wassawonariyanon abnormalbloodbacteriomegutdysbiosisandprogressiontoseveredenguedisease AT wassawonariyanon abnormalbloodbacteriomegutdysbiosisandprogressiontoseveredenguedisease AT vipathanachartwet abnormalbloodbacteriomegutdysbiosisandprogressiontoseveredenguedisease AT weerapongphumratanaprapin abnormalbloodbacteriomegutdysbiosisandprogressiontoseveredenguedisease AT polratwilairatana abnormalbloodbacteriomegutdysbiosisandprogressiontoseveredenguedisease AT asadaleelahavanichkul abnormalbloodbacteriomegutdysbiosisandprogressiontoseveredenguedisease AT asadaleelahavanichkul abnormalbloodbacteriomegutdysbiosisandprogressiontoseveredenguedisease |