Human peripheral blood mononuclear cells as an in vitro model for dengue virus infection = Descripción de un modelo de infección in vitro con virus dengue empleando células mononucleares humanas de sangre periférica
To date, there are no appropriate animal models for the study of the pathophysiology and clinical manifestations of the disease caused by dengue virus infection; therefore, experimental models are required for that purpose. The objective of the present work was to establish a model of in vitro infec...
| Main Authors: | , , |
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| Format: | Article |
| Language: | Spanish |
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Universidad de Antioquia
2014-07-01
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| Series: | Iatreia |
| Subjects: | |
| Online Access: | http://aprendeenlinea.udea.edu.co/revistas/index.php/iatreia/article/view/16221/16762 |
| _version_ | 1818912763681964032 |
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| author | Delgado Tiria, Felix Giovanni Pérez Acosta, Adriana Marisol Castellanos, Jaime E. |
| author_facet | Delgado Tiria, Felix Giovanni Pérez Acosta, Adriana Marisol Castellanos, Jaime E. |
| author_sort | Delgado Tiria, Felix Giovanni |
| collection | DOAJ |
| description | To date, there are no appropriate animal models for the study of the pathophysiology and clinical manifestations of the disease caused by dengue virus infection; therefore, experimental models are required for that purpose. The objective of the present work was to establish a model of in vitro infection with DENV-2. To this end, human peripheral blood mononuclear cells (PBMC) were obtained using a Ficoll gradient, and infected with DENV-2 using a low multiplicity of infection. The cell populations infected and responsible for the production of cytokines were identified using a multiparametric analysis by flow cytometry. As a result, PBMC were permissive to infection that was detected 24 hours after virus inoculation. Additionally, at this same time, CD14+ cells, but not CD3+ or CD19+ cells, were preferentially infected and responsible for the production of TNF-α and IL-6. In conclusion, we established a model of in vitro infection using unfractionated PBMC, in which CD14+ cells were identified as the primary target cells for infection with DENV-2, and the production of proinflammatory cytokines. |
| first_indexed | 2024-12-19T23:19:46Z |
| format | Article |
| id | doaj.art-ea0ba34b679b4442b0d2bdd7f7ab95aa |
| institution | Directory Open Access Journal |
| issn | 0121-0793 2011-7965 |
| language | Spanish |
| last_indexed | 2024-12-19T23:19:46Z |
| publishDate | 2014-07-01 |
| publisher | Universidad de Antioquia |
| record_format | Article |
| series | Iatreia |
| spelling | doaj.art-ea0ba34b679b4442b0d2bdd7f7ab95aa2022-12-21T20:02:00ZspaUniversidad de AntioquiaIatreia0121-07932011-79652014-07-01273267276Human peripheral blood mononuclear cells as an in vitro model for dengue virus infection = Descripción de un modelo de infección in vitro con virus dengue empleando células mononucleares humanas de sangre periféricaDelgado Tiria, Felix Giovanni 0Pérez Acosta, Adriana Marisol 1Castellanos, Jaime E. 2Universidad del BosqueUniversidad del BosqueUniversidad del BosqueTo date, there are no appropriate animal models for the study of the pathophysiology and clinical manifestations of the disease caused by dengue virus infection; therefore, experimental models are required for that purpose. The objective of the present work was to establish a model of in vitro infection with DENV-2. To this end, human peripheral blood mononuclear cells (PBMC) were obtained using a Ficoll gradient, and infected with DENV-2 using a low multiplicity of infection. The cell populations infected and responsible for the production of cytokines were identified using a multiparametric analysis by flow cytometry. As a result, PBMC were permissive to infection that was detected 24 hours after virus inoculation. Additionally, at this same time, CD14+ cells, but not CD3+ or CD19+ cells, were preferentially infected and responsible for the production of TNF-α and IL-6. In conclusion, we established a model of in vitro infection using unfractionated PBMC, in which CD14+ cells were identified as the primary target cells for infection with DENV-2, and the production of proinflammatory cytokines.http://aprendeenlinea.udea.edu.co/revistas/index.php/iatreia/article/view/16221/16762TNF-αDengue VirusFlow CytometryIL-6; Mononuclear CellsCélulas MononuclearesCitometría de FlujoIL-6Virus Dengue |
| spellingShingle | Delgado Tiria, Felix Giovanni Pérez Acosta, Adriana Marisol Castellanos, Jaime E. Human peripheral blood mononuclear cells as an in vitro model for dengue virus infection = Descripción de un modelo de infección in vitro con virus dengue empleando células mononucleares humanas de sangre periférica Iatreia TNF-α Dengue Virus Flow Cytometry IL-6; Mononuclear Cells Células Mononucleares Citometría de Flujo IL-6 Virus Dengue |
| title | Human peripheral blood mononuclear cells as an in vitro model for dengue virus infection = Descripción de un modelo de infección in vitro con virus dengue empleando células mononucleares humanas de sangre periférica |
| title_full | Human peripheral blood mononuclear cells as an in vitro model for dengue virus infection = Descripción de un modelo de infección in vitro con virus dengue empleando células mononucleares humanas de sangre periférica |
| title_fullStr | Human peripheral blood mononuclear cells as an in vitro model for dengue virus infection = Descripción de un modelo de infección in vitro con virus dengue empleando células mononucleares humanas de sangre periférica |
| title_full_unstemmed | Human peripheral blood mononuclear cells as an in vitro model for dengue virus infection = Descripción de un modelo de infección in vitro con virus dengue empleando células mononucleares humanas de sangre periférica |
| title_short | Human peripheral blood mononuclear cells as an in vitro model for dengue virus infection = Descripción de un modelo de infección in vitro con virus dengue empleando células mononucleares humanas de sangre periférica |
| title_sort | human peripheral blood mononuclear cells as an in vitro model for dengue virus infection descripcion de un modelo de infeccion in vitro con virus dengue empleando celulas mononucleares humanas de sangre periferica |
| topic | TNF-α Dengue Virus Flow Cytometry IL-6; Mononuclear Cells Células Mononucleares Citometría de Flujo IL-6 Virus Dengue |
| url | http://aprendeenlinea.udea.edu.co/revistas/index.php/iatreia/article/view/16221/16762 |
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