Towards the Physiological Understanding of <i>Yarrowia lipolytica</i> Growth and Lipase Production Using Waste Cooking Oils
The yeast <i>Yarrowia lipolytica</i> is an industrially relevant microorganism, which is able to convert low-value wastes into different high-value, bio-based products, such as enzymes, lipids, and other important metabolites. Waste cooking oil (WCO) represents one of the main streams ge...
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2022-07-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/15/14/5217 |
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| author | Mattia Colacicco Cosetta Ciliberti Gennaro Agrimi Antonino Biundo Isabella Pisano |
| author_facet | Mattia Colacicco Cosetta Ciliberti Gennaro Agrimi Antonino Biundo Isabella Pisano |
| author_sort | Mattia Colacicco |
| collection | DOAJ |
| description | The yeast <i>Yarrowia lipolytica</i> is an industrially relevant microorganism, which is able to convert low-value wastes into different high-value, bio-based products, such as enzymes, lipids, and other important metabolites. Waste cooking oil (WCO) represents one of the main streams generated in the food supply chain, especially from the domestic sector. The need to avoid its incorrect disposal makes this waste a resource for developing bioprocesses in the perspective of a circular bioeconomy. To this end, the strain <i>Y. lipolytica</i> W29 was used as a platform for the simultaneous production of intracellular lipids and extracellular lipases. Three different minimal media conditions with different pH controls were utilized in a small-scale (50 mL final volume) screening strategy, and the best condition was tested for an up-scaling procedure in higher volumes (800 mL) by selecting the best-performing possibility. The tested media were constituted by YNB media with high nitrogen restriction (1 g L<sup>−1</sup> (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>) and different carbon sources (3% <i>w v</i><sup>−1</sup> glucose and 10% <i>v v</i><sup>−1</sup> WCO) with different levels of pH controls. Lipase production and SCO content were analyzed. A direct correlation was found between decreasing FFA availability in the media and increasing SCO levels and lipase activity. The simultaneous production of extracellular lipase (1.164 ± 0.025 U mL<sup>−1</sup>) and intracellular single-cell oil accumulation by <i>Y. lipolytica</i> W29 growing on WCO demonstrates the potential and the industrial relevance of this biorefinery model. |
| first_indexed | 2024-03-09T03:27:54Z |
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| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-09T03:27:54Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-ea0d9fb0e99b4f688b08054f2f60e6212023-12-03T14:59:45ZengMDPI AGEnergies1996-10732022-07-011514521710.3390/en15145217Towards the Physiological Understanding of <i>Yarrowia lipolytica</i> Growth and Lipase Production Using Waste Cooking OilsMattia Colacicco0Cosetta Ciliberti1Gennaro Agrimi2Antonino Biundo3Isabella Pisano4Department of Bioscience, Biotechnology and Biopharmaceutics, University of Bari, Via Edoardo Orabona, 4, 70125 Bari, ItalyDepartment of Bioscience, Biotechnology and Biopharmaceutics, University of Bari, Via Edoardo Orabona, 4, 70125 Bari, ItalyDepartment of Bioscience, Biotechnology and Biopharmaceutics, University of Bari, Via Edoardo Orabona, 4, 70125 Bari, ItalyDepartment of Bioscience, Biotechnology and Biopharmaceutics, University of Bari, Via Edoardo Orabona, 4, 70125 Bari, ItalyDepartment of Bioscience, Biotechnology and Biopharmaceutics, University of Bari, Via Edoardo Orabona, 4, 70125 Bari, ItalyThe yeast <i>Yarrowia lipolytica</i> is an industrially relevant microorganism, which is able to convert low-value wastes into different high-value, bio-based products, such as enzymes, lipids, and other important metabolites. Waste cooking oil (WCO) represents one of the main streams generated in the food supply chain, especially from the domestic sector. The need to avoid its incorrect disposal makes this waste a resource for developing bioprocesses in the perspective of a circular bioeconomy. To this end, the strain <i>Y. lipolytica</i> W29 was used as a platform for the simultaneous production of intracellular lipids and extracellular lipases. Three different minimal media conditions with different pH controls were utilized in a small-scale (50 mL final volume) screening strategy, and the best condition was tested for an up-scaling procedure in higher volumes (800 mL) by selecting the best-performing possibility. The tested media were constituted by YNB media with high nitrogen restriction (1 g L<sup>−1</sup> (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>) and different carbon sources (3% <i>w v</i><sup>−1</sup> glucose and 10% <i>v v</i><sup>−1</sup> WCO) with different levels of pH controls. Lipase production and SCO content were analyzed. A direct correlation was found between decreasing FFA availability in the media and increasing SCO levels and lipase activity. The simultaneous production of extracellular lipase (1.164 ± 0.025 U mL<sup>−1</sup>) and intracellular single-cell oil accumulation by <i>Y. lipolytica</i> W29 growing on WCO demonstrates the potential and the industrial relevance of this biorefinery model.https://www.mdpi.com/1996-1073/15/14/5217<i>Yarrowia lipolytica</i>single-cell oilslipasewaste cooking oilcircular bioeconomybiorefinery |
| spellingShingle | Mattia Colacicco Cosetta Ciliberti Gennaro Agrimi Antonino Biundo Isabella Pisano Towards the Physiological Understanding of <i>Yarrowia lipolytica</i> Growth and Lipase Production Using Waste Cooking Oils Energies <i>Yarrowia lipolytica</i> single-cell oils lipase waste cooking oil circular bioeconomy biorefinery |
| title | Towards the Physiological Understanding of <i>Yarrowia lipolytica</i> Growth and Lipase Production Using Waste Cooking Oils |
| title_full | Towards the Physiological Understanding of <i>Yarrowia lipolytica</i> Growth and Lipase Production Using Waste Cooking Oils |
| title_fullStr | Towards the Physiological Understanding of <i>Yarrowia lipolytica</i> Growth and Lipase Production Using Waste Cooking Oils |
| title_full_unstemmed | Towards the Physiological Understanding of <i>Yarrowia lipolytica</i> Growth and Lipase Production Using Waste Cooking Oils |
| title_short | Towards the Physiological Understanding of <i>Yarrowia lipolytica</i> Growth and Lipase Production Using Waste Cooking Oils |
| title_sort | towards the physiological understanding of i yarrowia lipolytica i growth and lipase production using waste cooking oils |
| topic | <i>Yarrowia lipolytica</i> single-cell oils lipase waste cooking oil circular bioeconomy biorefinery |
| url | https://www.mdpi.com/1996-1073/15/14/5217 |
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