Fluorescent diagnostics of benign breast diseases and breast cancer
In the study the spectra of fluorescence excitation and autofluorescence of histological sections with different pathological processes in breast were measured. Additionally, element composition of the tissues was investigated by scanning electron microscopy. It has been shown that spectra of fluore...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Samara National Research University
2017-12-01
|
| Series: | Journal of Biomedical Photonics & Engineering |
| Subjects: | |
| Online Access: | http://jbpe.ssau.ru/index.php/JBPE/article/view/3271 |
| _version_ | 1828233413439520768 |
|---|---|
| author | Valeriya S. Maryakhina Yulia S. Korneva Igor V. Chekurov Oksana A. Shisterova |
| author_facet | Valeriya S. Maryakhina Yulia S. Korneva Igor V. Chekurov Oksana A. Shisterova |
| author_sort | Valeriya S. Maryakhina |
| collection | DOAJ |
| description | In the study the spectra of fluorescence excitation and autofluorescence of histological sections with different pathological processes in breast were measured. Additionally, element composition of the tissues was investigated by scanning electron microscopy. It has been shown that spectra of fluorescence excitation of benign breast diseases (fibroadenoma and fibrocystic breast disease) have low maximum at 232 nm and high maximum at 260 nm. Also, fluorescence spectra of histological samples change during tumor growth and have two maxima (335 and 420 nm). The registered spectra are result of emission of such fluorophores as fatty acids, tryptophan, NADH, vitamin B6, collagen and bilirubin. The obtained spectral data correlate well with data of element analysis. Selenium status of the tissues was investigated by scanning electronic microscope. High concentration if selenium was detected only in fibroadenoma. In breast cancer samples and fibrocystic breast disease low selenium content was detected. It may confirm higher risk for malignant transformation of fibrocystic breast disease than fibroadenoma. Our results may be helpful for cancer diagnostics and for prognosis prediction. |
| first_indexed | 2024-04-12T19:39:14Z |
| format | Article |
| id | doaj.art-ea0ee26974044a04abce13baeb61a599 |
| institution | Directory Open Access Journal |
| issn | 2411-2844 |
| language | English |
| last_indexed | 2024-04-12T19:39:14Z |
| publishDate | 2017-12-01 |
| publisher | Samara National Research University |
| record_format | Article |
| series | Journal of Biomedical Photonics & Engineering |
| spelling | doaj.art-ea0ee26974044a04abce13baeb61a5992022-12-22T03:19:08ZengSamara National Research UniversityJournal of Biomedical Photonics & Engineering2411-28442017-12-013410.18287/JBPE17.03.0403062957Fluorescent diagnostics of benign breast diseases and breast cancerValeriya S. Maryakhina0Yulia S. Korneva1Igor V. Chekurov2Oksana A. Shisterova3Kumertau branch of Orenburg State University, Kumertau, RussiaSmolensk State Medical University, Russia; Smolensk Regional Institute of Pathology, RussiaOrenburg State Agriculture University, RussiaSmolensk Oncological Dispensary, RussiaIn the study the spectra of fluorescence excitation and autofluorescence of histological sections with different pathological processes in breast were measured. Additionally, element composition of the tissues was investigated by scanning electron microscopy. It has been shown that spectra of fluorescence excitation of benign breast diseases (fibroadenoma and fibrocystic breast disease) have low maximum at 232 nm and high maximum at 260 nm. Also, fluorescence spectra of histological samples change during tumor growth and have two maxima (335 and 420 nm). The registered spectra are result of emission of such fluorophores as fatty acids, tryptophan, NADH, vitamin B6, collagen and bilirubin. The obtained spectral data correlate well with data of element analysis. Selenium status of the tissues was investigated by scanning electronic microscope. High concentration if selenium was detected only in fibroadenoma. In breast cancer samples and fibrocystic breast disease low selenium content was detected. It may confirm higher risk for malignant transformation of fibrocystic breast disease than fibroadenoma. Our results may be helpful for cancer diagnostics and for prognosis prediction.http://jbpe.ssau.ru/index.php/JBPE/article/view/3271breast cancerbenign breast diseasefluorescenceoptical biomedical diagnostics |
| spellingShingle | Valeriya S. Maryakhina Yulia S. Korneva Igor V. Chekurov Oksana A. Shisterova Fluorescent diagnostics of benign breast diseases and breast cancer Journal of Biomedical Photonics & Engineering breast cancer benign breast disease fluorescence optical biomedical diagnostics |
| title | Fluorescent diagnostics of benign breast diseases and breast cancer |
| title_full | Fluorescent diagnostics of benign breast diseases and breast cancer |
| title_fullStr | Fluorescent diagnostics of benign breast diseases and breast cancer |
| title_full_unstemmed | Fluorescent diagnostics of benign breast diseases and breast cancer |
| title_short | Fluorescent diagnostics of benign breast diseases and breast cancer |
| title_sort | fluorescent diagnostics of benign breast diseases and breast cancer |
| topic | breast cancer benign breast disease fluorescence optical biomedical diagnostics |
| url | http://jbpe.ssau.ru/index.php/JBPE/article/view/3271 |
| work_keys_str_mv | AT valeriyasmaryakhina fluorescentdiagnosticsofbenignbreastdiseasesandbreastcancer AT yuliaskorneva fluorescentdiagnosticsofbenignbreastdiseasesandbreastcancer AT igorvchekurov fluorescentdiagnosticsofbenignbreastdiseasesandbreastcancer AT oksanaashisterova fluorescentdiagnosticsofbenignbreastdiseasesandbreastcancer |