Association of nine pathobionts with periodontitis in four South American and European countries
ABSTRACTAim Our aim was to compare the prevalence and load of nine pathobionts in subgingival samples of healthy individuals and periodontitis patients from four different countries.Methods Five hundred and seven subgingival biofilm samples were collected from healthy subjects and periodontitis pati...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2023.2188630 |
| _version_ | 1797449996595888128 |
|---|---|
| author | Gerard Àlvarez Alexandre Arredondo Sergio Isabal Wim Teughels Isabelle Laleman María José Contreras Lorena Isbej Enrique Huapaya Gerardo Mendoza Carolina Mor José Nart Vanessa Blanc Rubén León |
| author_facet | Gerard Àlvarez Alexandre Arredondo Sergio Isabal Wim Teughels Isabelle Laleman María José Contreras Lorena Isbej Enrique Huapaya Gerardo Mendoza Carolina Mor José Nart Vanessa Blanc Rubén León |
| author_sort | Gerard Àlvarez |
| collection | DOAJ |
| description | ABSTRACTAim Our aim was to compare the prevalence and load of nine pathobionts in subgingival samples of healthy individuals and periodontitis patients from four different countries.Methods Five hundred and seven subgingival biofilm samples were collected from healthy subjects and periodontitis patients in Belgium, Chile, Peru and Spain. The prevalence and load of Eubacterium brachy, Filifactor alocis, Fretibacterium fastidiosum, Porphyromonas endodontalis, Porphyromonas gingivalis, Selenomonas sputigena, Treponema denticola, Tannerella forsythia and Treponema socranskii were measured by quantitative PCR.Results The association with periodontitis of all species, except for T. socranskii, was confirmed in all countries but Peru, where only P. endodontalis, P. gingivalis and T. denticola were found to be significantly associated. Moreover, most species showed higher loads at greater CAL and PPD, but not where there was BOP. Through Principal Component Analysis, samples showed clearly different distributions by diagnosis, despite observing a smaller separation in Peruvian samples.Conclusions Unlike prevalence, relative load was found to be a reliable variable to discriminate the association of the species with periodontitis. Based on this, F. alocis, P. endodontalis, P. gingivalis, T. denticola and T. forsythia may be biomarkers of disease in Belgium, Chile and Spain, due to their significantly higher abundance in periodontitis patients. |
| first_indexed | 2024-03-09T14:33:11Z |
| format | Article |
| id | doaj.art-ea1037b15d624c82ab7add927e49fffe |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-03-09T14:33:11Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-ea1037b15d624c82ab7add927e49fffe2023-11-27T17:25:00ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972023-12-0115110.1080/20002297.2023.2188630Association of nine pathobionts with periodontitis in four South American and European countriesGerard Àlvarez0Alexandre Arredondo1Sergio Isabal2Wim Teughels3Isabelle Laleman4María José Contreras5Lorena Isbej6Enrique Huapaya7Gerardo Mendoza8Carolina Mor9José Nart10Vanessa Blanc11Rubén León12Department of Microbiology, DENTAID Research Center, Cerdanyola del Vallès, SpainDepartment of Microbiology, DENTAID Research Center, Cerdanyola del Vallès, SpainDepartment of Microbiology, DENTAID Research Center, Cerdanyola del Vallès, SpainDepartment of Oral Health Sciences, KU Leuven, Belgium & Dentistry, University Hospitals Leuven, Leuven, BelgiumDepartment of Oral Health Sciences, KU Leuven, Belgium & Dentistry, University Hospitals Leuven, Leuven, BelgiumSchool of Dentistry, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, ChileSchool of Dentistry, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, ChileDepartment of Periodontology, School of Dentistry, Universidad Científica del Sur, Lima, PeruDepartment of Periodontology, School of Dentistry, Universidad Científica del Sur, Lima, PeruDepartment of Periodontology, Universitat Internacional de Catalunya, Barcelona, SpainDepartment of Periodontology, Universitat Internacional de Catalunya, Barcelona, SpainDepartment of Microbiology, DENTAID Research Center, Cerdanyola del Vallès, SpainDepartment of Microbiology, DENTAID Research Center, Cerdanyola del Vallès, SpainABSTRACTAim Our aim was to compare the prevalence and load of nine pathobionts in subgingival samples of healthy individuals and periodontitis patients from four different countries.Methods Five hundred and seven subgingival biofilm samples were collected from healthy subjects and periodontitis patients in Belgium, Chile, Peru and Spain. The prevalence and load of Eubacterium brachy, Filifactor alocis, Fretibacterium fastidiosum, Porphyromonas endodontalis, Porphyromonas gingivalis, Selenomonas sputigena, Treponema denticola, Tannerella forsythia and Treponema socranskii were measured by quantitative PCR.Results The association with periodontitis of all species, except for T. socranskii, was confirmed in all countries but Peru, where only P. endodontalis, P. gingivalis and T. denticola were found to be significantly associated. Moreover, most species showed higher loads at greater CAL and PPD, but not where there was BOP. Through Principal Component Analysis, samples showed clearly different distributions by diagnosis, despite observing a smaller separation in Peruvian samples.Conclusions Unlike prevalence, relative load was found to be a reliable variable to discriminate the association of the species with periodontitis. Based on this, F. alocis, P. endodontalis, P. gingivalis, T. denticola and T. forsythia may be biomarkers of disease in Belgium, Chile and Spain, due to their significantly higher abundance in periodontitis patients.https://www.tandfonline.com/doi/10.1080/20002297.2023.2188630Bacterial loadbiomarkerpathobiontprevalencequantitative PCRspecies specificity |
| spellingShingle | Gerard Àlvarez Alexandre Arredondo Sergio Isabal Wim Teughels Isabelle Laleman María José Contreras Lorena Isbej Enrique Huapaya Gerardo Mendoza Carolina Mor José Nart Vanessa Blanc Rubén León Association of nine pathobionts with periodontitis in four South American and European countries Journal of Oral Microbiology Bacterial load biomarker pathobiont prevalence quantitative PCR species specificity |
| title | Association of nine pathobionts with periodontitis in four South American and European countries |
| title_full | Association of nine pathobionts with periodontitis in four South American and European countries |
| title_fullStr | Association of nine pathobionts with periodontitis in four South American and European countries |
| title_full_unstemmed | Association of nine pathobionts with periodontitis in four South American and European countries |
| title_short | Association of nine pathobionts with periodontitis in four South American and European countries |
| title_sort | association of nine pathobionts with periodontitis in four south american and european countries |
| topic | Bacterial load biomarker pathobiont prevalence quantitative PCR species specificity |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2023.2188630 |
| work_keys_str_mv | AT gerardalvarez associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT alexandrearredondo associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT sergioisabal associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT wimteughels associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT isabellelaleman associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT mariajosecontreras associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT lorenaisbej associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT enriquehuapaya associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT gerardomendoza associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT carolinamor associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT josenart associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT vanessablanc associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries AT rubenleon associationofninepathobiontswithperiodontitisinfoursouthamericanandeuropeancountries |