Abnormal expression of TBX4 during anorectal development in rat embryos with ethylenethiourea-induced anorectal malformations
Abstract Background To assess the expression of T-box transcription factor 4 (TBX4) during the anorectal development in normal and ethylenethiourea (ETU)-induced anorectal malformations (ARM) rat embryos. Methods Anorectal malformations was induced by ETU on the 10th gestational day (E10) in rat emb...
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BMC
2019-05-01
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| Series: | Biological Research |
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| Online Access: | http://link.springer.com/article/10.1186/s40659-019-0235-6 |
| _version_ | 1818450942739087360 |
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| author | Meng Li Hailan Zhang Huiying Liu Hongzhong Tian Xiaobing Tang Yuzuo Bai Weilin Wang |
| author_facet | Meng Li Hailan Zhang Huiying Liu Hongzhong Tian Xiaobing Tang Yuzuo Bai Weilin Wang |
| author_sort | Meng Li |
| collection | DOAJ |
| description | Abstract Background To assess the expression of T-box transcription factor 4 (TBX4) during the anorectal development in normal and ethylenethiourea (ETU)-induced anorectal malformations (ARM) rat embryos. Methods Anorectal malformations was induced by ETU on the 10th gestational day (E10) in rat embryos. Spatio-temporal expression of TBX4 was evaluated in normal (n = 490) and ETU-induced ARM rat embryos (n = 455) from E13 to E16 by immunohistochemical staining, Western blot analysis and real-time RT-PCR. Results In the normal embryos, immunohistochemical staining revealed that TBX4 expression was detected in the epithelium of hindgut and urorectal septum (URS) on E13. TBX4-immunopositive cells were increased significantly in the epithelium of hindgut and URS, the future anal orifice part of cloacal membrane on E14. On E15, abundant stained cells were observed in the rectum, URS and dorsal cloacal membrane and the expression of positive cells reached its peak. On E16, only sporadic positive cells were distributed in the epithelium of the distal rectum. In the ARM embryos, the hindgut/rectum, URS and dorsal cloacal membrane were faint for TBX4 immunohistochemical staining. In the normal group, TBX4 protein and mRNA expression showed time-dependent changes in the hindgut/rectum from E13 to E16 on Western blot and real-time RT-PCR. On E13 and E15, the expression level of TBX4 mRNA in the ARM group was significantly lower than that in the normal group (P < 0.05). On E15, the expression level of TBX4 protein in the ARM group was significantly lower than that in the normal group (P < 0.05). Conclusions The expression of TBX4 was downregulated in ETU-induced ARM embryos, which may play important roles in the pathogenesis of anorectal development. |
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| institution | Directory Open Access Journal |
| issn | 0717-6287 |
| language | English |
| last_indexed | 2024-12-14T20:59:19Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
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| series | Biological Research |
| spelling | doaj.art-ea1438432b634275ae29fac0dcde00372022-12-21T22:47:37ZengBMCBiological Research0717-62872019-05-015211910.1186/s40659-019-0235-6Abnormal expression of TBX4 during anorectal development in rat embryos with ethylenethiourea-induced anorectal malformationsMeng Li0Hailan Zhang1Huiying Liu2Hongzhong Tian3Xiaobing Tang4Yuzuo Bai5Weilin Wang6Department of Pediatric Surgery, Shengjing Hospital of China Medical UniversityDepartment of Pediatric Surgery, Shengjing Hospital of China Medical UniversityDepartment of Pediatric Surgery, Shengjing Hospital of China Medical UniversityDepartment of Pediatric Surgery, Shengjing Hospital of China Medical UniversityDepartment of Pediatric Surgery, Shengjing Hospital of China Medical UniversityDepartment of Pediatric Surgery, Shengjing Hospital of China Medical UniversityDepartment of Pediatric Surgery, Shengjing Hospital of China Medical UniversityAbstract Background To assess the expression of T-box transcription factor 4 (TBX4) during the anorectal development in normal and ethylenethiourea (ETU)-induced anorectal malformations (ARM) rat embryos. Methods Anorectal malformations was induced by ETU on the 10th gestational day (E10) in rat embryos. Spatio-temporal expression of TBX4 was evaluated in normal (n = 490) and ETU-induced ARM rat embryos (n = 455) from E13 to E16 by immunohistochemical staining, Western blot analysis and real-time RT-PCR. Results In the normal embryos, immunohistochemical staining revealed that TBX4 expression was detected in the epithelium of hindgut and urorectal septum (URS) on E13. TBX4-immunopositive cells were increased significantly in the epithelium of hindgut and URS, the future anal orifice part of cloacal membrane on E14. On E15, abundant stained cells were observed in the rectum, URS and dorsal cloacal membrane and the expression of positive cells reached its peak. On E16, only sporadic positive cells were distributed in the epithelium of the distal rectum. In the ARM embryos, the hindgut/rectum, URS and dorsal cloacal membrane were faint for TBX4 immunohistochemical staining. In the normal group, TBX4 protein and mRNA expression showed time-dependent changes in the hindgut/rectum from E13 to E16 on Western blot and real-time RT-PCR. On E13 and E15, the expression level of TBX4 mRNA in the ARM group was significantly lower than that in the normal group (P < 0.05). On E15, the expression level of TBX4 protein in the ARM group was significantly lower than that in the normal group (P < 0.05). Conclusions The expression of TBX4 was downregulated in ETU-induced ARM embryos, which may play important roles in the pathogenesis of anorectal development.http://link.springer.com/article/10.1186/s40659-019-0235-6Anorectal malformationsTBX4Embryogenesis |
| spellingShingle | Meng Li Hailan Zhang Huiying Liu Hongzhong Tian Xiaobing Tang Yuzuo Bai Weilin Wang Abnormal expression of TBX4 during anorectal development in rat embryos with ethylenethiourea-induced anorectal malformations Biological Research Anorectal malformations TBX4 Embryogenesis |
| title | Abnormal expression of TBX4 during anorectal development in rat embryos with ethylenethiourea-induced anorectal malformations |
| title_full | Abnormal expression of TBX4 during anorectal development in rat embryos with ethylenethiourea-induced anorectal malformations |
| title_fullStr | Abnormal expression of TBX4 during anorectal development in rat embryos with ethylenethiourea-induced anorectal malformations |
| title_full_unstemmed | Abnormal expression of TBX4 during anorectal development in rat embryos with ethylenethiourea-induced anorectal malformations |
| title_short | Abnormal expression of TBX4 during anorectal development in rat embryos with ethylenethiourea-induced anorectal malformations |
| title_sort | abnormal expression of tbx4 during anorectal development in rat embryos with ethylenethiourea induced anorectal malformations |
| topic | Anorectal malformations TBX4 Embryogenesis |
| url | http://link.springer.com/article/10.1186/s40659-019-0235-6 |
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