Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations
A high performance liquid chromatographic (HPLC) method with diode array detection (DAD) was established for simultaneous determination of seven main bioactive components in San-ao decoction and its series of formulae (San-ao decoction, Wu-ao decoction, Qi-ao decoction and Jia-wei San-ao decoction)....
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MDPI AG
2012-11-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/11/12925 |
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| author | Anwei Ding Xinshen Fan Erxing Shang Yuping Tang Chenxue Jiang Xiaoyun Shu |
| author_facet | Anwei Ding Xinshen Fan Erxing Shang Yuping Tang Chenxue Jiang Xiaoyun Shu |
| author_sort | Anwei Ding |
| collection | DOAJ |
| description | A high performance liquid chromatographic (HPLC) method with diode array detection (DAD) was established for simultaneous determination of seven main bioactive components in San-ao decoction and its series of formulae (San-ao decoction, Wu-ao decoction, Qi-ao decoction and Jia-wei San-ao decoction). Seven compounds were analyzed simultaneously with a XTerra C18 column (4.6 mm × 250 mm, 5 µm) using a linear gradient elution of a mobile phase containing acetonitrile (A) and a buffer solution (0.02 mol/L potassium dihydrogen phosphate and adjusted to pH 3 using phosphoric acid) (B); the flow rate was 1.0 mL/min. The sample was detected with DAD at 210, 254 and 360 nm and the column was maintained at 30 °C. All the compounds showed good linearity (r2 > 0.9984) in the tested concentration range. The precisions were evaluated by intra-day and inter-day tests, and relative standard deviation (R.S.D.) values within the range of 0.83%–2.53% and 0.64%–2.77% were reported, respectively. The recoveries of the quantified compounds were observed to cover a range from 95.34% and 104.82% with R.S.D. values less than 2.72%. The validated method was successfully applied for the simultaneous determination of seven main bioactive components including ephedrine (1), amygdalin (2), liquiritin (3), benzoic acid (4), isoliquiritin (5), formononetin (6) and glycyrrhizic acid (7) in San-ao decoction and its series of formulae. The results also showed a wide variation in the content of the identified active compounds in these samples, which could also be helpful to illustrate the drug interactions after some herbs combined in different formulations. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T09:22:02Z |
| publishDate | 2012-11-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj.art-ea147c9fcef640d2854470cb067d2cd42022-12-22T01:13:15ZengMDPI AGMolecules1420-30492012-11-011711129251293710.3390/molecules171112925Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of FormulationsAnwei DingXinshen FanErxing ShangYuping TangChenxue JiangXiaoyun ShuA high performance liquid chromatographic (HPLC) method with diode array detection (DAD) was established for simultaneous determination of seven main bioactive components in San-ao decoction and its series of formulae (San-ao decoction, Wu-ao decoction, Qi-ao decoction and Jia-wei San-ao decoction). Seven compounds were analyzed simultaneously with a XTerra C18 column (4.6 mm × 250 mm, 5 µm) using a linear gradient elution of a mobile phase containing acetonitrile (A) and a buffer solution (0.02 mol/L potassium dihydrogen phosphate and adjusted to pH 3 using phosphoric acid) (B); the flow rate was 1.0 mL/min. The sample was detected with DAD at 210, 254 and 360 nm and the column was maintained at 30 °C. All the compounds showed good linearity (r2 > 0.9984) in the tested concentration range. The precisions were evaluated by intra-day and inter-day tests, and relative standard deviation (R.S.D.) values within the range of 0.83%–2.53% and 0.64%–2.77% were reported, respectively. The recoveries of the quantified compounds were observed to cover a range from 95.34% and 104.82% with R.S.D. values less than 2.72%. The validated method was successfully applied for the simultaneous determination of seven main bioactive components including ephedrine (1), amygdalin (2), liquiritin (3), benzoic acid (4), isoliquiritin (5), formononetin (6) and glycyrrhizic acid (7) in San-ao decoction and its series of formulae. The results also showed a wide variation in the content of the identified active compounds in these samples, which could also be helpful to illustrate the drug interactions after some herbs combined in different formulations.http://www.mdpi.com/1420-3049/17/11/12925San-ao decoctionformulationsEphedrae HerbaArmeniacae Semen AmarumGlycyrrhizae Radix et RhizomaHPLC-DAD |
| spellingShingle | Anwei Ding Xinshen Fan Erxing Shang Yuping Tang Chenxue Jiang Xiaoyun Shu Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations Molecules San-ao decoction formulations Ephedrae Herba Armeniacae Semen Amarum Glycyrrhizae Radix et Rhizoma HPLC-DAD |
| title | Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations |
| title_full | Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations |
| title_fullStr | Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations |
| title_full_unstemmed | Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations |
| title_short | Comparative Analysis of the Main Bioactive Components of San-ao Decoction and Its Series of Formulations |
| title_sort | comparative analysis of the main bioactive components of san ao decoction and its series of formulations |
| topic | San-ao decoction formulations Ephedrae Herba Armeniacae Semen Amarum Glycyrrhizae Radix et Rhizoma HPLC-DAD |
| url | http://www.mdpi.com/1420-3049/17/11/12925 |
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