Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial waste
Lipase is one of the most used industrial enzymes, which can be produced by Aspergillus niger. Large scale production of Aspergillus niger lipase is more profitable by using solid state fermentation method and utilizing agro-industrial waste as the substrate. Optimization of Aspergillus niger lipase...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-02-01
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| Series: | Energy Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352484719306584 |
| _version_ | 1828813103678095360 |
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| author | Dwini Normayulisa Putri Andy Khootama Meka Saima Perdani Tania Surya Utami Heri Hermansyah |
| author_facet | Dwini Normayulisa Putri Andy Khootama Meka Saima Perdani Tania Surya Utami Heri Hermansyah |
| author_sort | Dwini Normayulisa Putri |
| collection | DOAJ |
| description | Lipase is one of the most used industrial enzymes, which can be produced by Aspergillus niger. Large scale production of Aspergillus niger lipase is more profitable by using solid state fermentation method and utilizing agro-industrial waste as the substrate. Optimization of Aspergillus niger lipase production has been performed in this study. Optimization was performed on solid state fermentation of rice bran and Jathropa seed cake for 5 days with variations of inducer and extractant. Fermentation cake produced was extracted, filtered using muslin cloth, and centrifuged. The supernatant was spray-dried and assayed using olive oil hydrolysis titrimetry. Inducer optimization results showed that 1% of olive oil was the best inducer, yielding dry lipase extract with highest activity unit (176 U/ml enzyme). Extractant optimization results showed that 1% of NaCl – 0.5% of Tween 80 was the best extractant, yielding dry lipase extract with highest activity unit (282 U/ml enzyme). Keywords: Agro-industrial waste, Aspergillus niger, Lipase, Solid-state fermentation |
| first_indexed | 2024-12-12T09:54:25Z |
| format | Article |
| id | doaj.art-ea15307860984d17a8745aa2ed0485df |
| institution | Directory Open Access Journal |
| issn | 2352-4847 |
| language | English |
| last_indexed | 2024-12-12T09:54:25Z |
| publishDate | 2020-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Energy Reports |
| spelling | doaj.art-ea15307860984d17a8745aa2ed0485df2022-12-22T00:28:11ZengElsevierEnergy Reports2352-48472020-02-016331335Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial wasteDwini Normayulisa Putri0Andy Khootama1Meka Saima Perdani2Tania Surya Utami3Heri Hermansyah4Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, IndonesiaDepartment of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, IndonesiaCorresponding author.; Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, IndonesiaLipase is one of the most used industrial enzymes, which can be produced by Aspergillus niger. Large scale production of Aspergillus niger lipase is more profitable by using solid state fermentation method and utilizing agro-industrial waste as the substrate. Optimization of Aspergillus niger lipase production has been performed in this study. Optimization was performed on solid state fermentation of rice bran and Jathropa seed cake for 5 days with variations of inducer and extractant. Fermentation cake produced was extracted, filtered using muslin cloth, and centrifuged. The supernatant was spray-dried and assayed using olive oil hydrolysis titrimetry. Inducer optimization results showed that 1% of olive oil was the best inducer, yielding dry lipase extract with highest activity unit (176 U/ml enzyme). Extractant optimization results showed that 1% of NaCl – 0.5% of Tween 80 was the best extractant, yielding dry lipase extract with highest activity unit (282 U/ml enzyme). Keywords: Agro-industrial waste, Aspergillus niger, Lipase, Solid-state fermentationhttp://www.sciencedirect.com/science/article/pii/S2352484719306584 |
| spellingShingle | Dwini Normayulisa Putri Andy Khootama Meka Saima Perdani Tania Surya Utami Heri Hermansyah Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial waste Energy Reports |
| title | Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial waste |
| title_full | Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial waste |
| title_fullStr | Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial waste |
| title_full_unstemmed | Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial waste |
| title_short | Optimization of Aspergillus niger lipase production by solid state fermentation of agro-industrial waste |
| title_sort | optimization of aspergillus niger lipase production by solid state fermentation of agro industrial waste |
| url | http://www.sciencedirect.com/science/article/pii/S2352484719306584 |
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