Cranberry Arabino-Xyloglucan and Pectic Oligosaccharides Induce <i>Lactobacillus</i> Growth and Short-Chain Fatty Acid Production
Numerous health benefits have been reported from the consumption of cranberry-derived products, and recent studies have identified bioactive polysaccharides and oligosaccharides from cranberry pomace. This study aimed to further characterize xyloglucan and pectic oligosaccharide structures from pect...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/7/1346 |
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| author | Arland T. Hotchkiss John A. Renye Andre K. White Alberto Nunez Giselle K. P. Guron Hoa Chau Stefanie Simon Carlos Poveda Gemma Walton Robert Rastall Christina Khoo |
| author_facet | Arland T. Hotchkiss John A. Renye Andre K. White Alberto Nunez Giselle K. P. Guron Hoa Chau Stefanie Simon Carlos Poveda Gemma Walton Robert Rastall Christina Khoo |
| author_sort | Arland T. Hotchkiss |
| collection | DOAJ |
| description | Numerous health benefits have been reported from the consumption of cranberry-derived products, and recent studies have identified bioactive polysaccharides and oligosaccharides from cranberry pomace. This study aimed to further characterize xyloglucan and pectic oligosaccharide structures from pectinase-treated cranberry pomace and measure the growth and short-chain fatty acid production of 86 <i>Lactobacillus</i> strains using a cranberry oligosaccharide fraction as the carbon source. In addition to arabino-xyloglucan structures, cranberry oligosaccharides included pectic rhamnogalacturonan I which was methyl-esterified, acetylated and contained arabino-galacto-oligosaccharide side chains and a 4,5-unsaturated function at the non-reducing end. When grown on cranberry oligosaccharides, ten <i>Lactobacillus</i> strains reached a final culture density (ΔOD) ≥ 0.50 after 24 h incubation at 32 °C, which was comparable to <i>L. plantarum</i> ATCC BAA 793. All strains produced lactic, acetic, and propionic acids, and all but three strains produced butyric acid. This study demonstrated that the ability to metabolize cranberry oligosaccharides is <i>Lactobacillus</i> strain specific, with some strains having the potential to be probiotics, and for the first time showed these ten strains were capable of growth on this carbon source. The novel cranberry pectic and arabino-xyloglucan oligosaccharide structures reported here combined with the <i>Lactobacillus</i> strains that can metabolize cranberry oligosaccharides and produce short-chain fatty acids, have excellent potential as health-promoting synbiotics. |
| first_indexed | 2024-03-09T10:14:53Z |
| format | Article |
| id | doaj.art-ea1a051ee3a84bc5be28ba8eae504fd6 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T10:14:53Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ea1a051ee3a84bc5be28ba8eae504fd62023-12-01T22:28:20ZengMDPI AGMicroorganisms2076-26072022-07-01107134610.3390/microorganisms10071346Cranberry Arabino-Xyloglucan and Pectic Oligosaccharides Induce <i>Lactobacillus</i> Growth and Short-Chain Fatty Acid ProductionArland T. Hotchkiss0John A. Renye1Andre K. White2Alberto Nunez3Giselle K. P. Guron4Hoa Chau5Stefanie Simon6Carlos Poveda7Gemma Walton8Robert Rastall9Christina Khoo10Dairy & Functional Foods Research Unit, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADairy & Functional Foods Research Unit, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADairy & Functional Foods Research Unit, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADairy & Functional Foods Research Unit, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADairy & Functional Foods Research Unit, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADairy & Functional Foods Research Unit, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USASustainable Biofuels and Co-Products Research Unit, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA 19038, USADepartment of Food and Nutritional Sciences, The University of Reading, Harry Nursten Building, Pepper Lane, Whiteknights, Reading RG6 6DZ, UKDepartment of Food and Nutritional Sciences, The University of Reading, Harry Nursten Building, Pepper Lane, Whiteknights, Reading RG6 6DZ, UKDepartment of Food and Nutritional Sciences, The University of Reading, Harry Nursten Building, Pepper Lane, Whiteknights, Reading RG6 6DZ, UKOcean Spray Cranberries, Inc., One Ocean Spray Drive, Lakeville-Middleboro, MA 02349, USANumerous health benefits have been reported from the consumption of cranberry-derived products, and recent studies have identified bioactive polysaccharides and oligosaccharides from cranberry pomace. This study aimed to further characterize xyloglucan and pectic oligosaccharide structures from pectinase-treated cranberry pomace and measure the growth and short-chain fatty acid production of 86 <i>Lactobacillus</i> strains using a cranberry oligosaccharide fraction as the carbon source. In addition to arabino-xyloglucan structures, cranberry oligosaccharides included pectic rhamnogalacturonan I which was methyl-esterified, acetylated and contained arabino-galacto-oligosaccharide side chains and a 4,5-unsaturated function at the non-reducing end. When grown on cranberry oligosaccharides, ten <i>Lactobacillus</i> strains reached a final culture density (ΔOD) ≥ 0.50 after 24 h incubation at 32 °C, which was comparable to <i>L. plantarum</i> ATCC BAA 793. All strains produced lactic, acetic, and propionic acids, and all but three strains produced butyric acid. This study demonstrated that the ability to metabolize cranberry oligosaccharides is <i>Lactobacillus</i> strain specific, with some strains having the potential to be probiotics, and for the first time showed these ten strains were capable of growth on this carbon source. The novel cranberry pectic and arabino-xyloglucan oligosaccharide structures reported here combined with the <i>Lactobacillus</i> strains that can metabolize cranberry oligosaccharides and produce short-chain fatty acids, have excellent potential as health-promoting synbiotics.https://www.mdpi.com/2076-2607/10/7/1346cranberryxyloglucanrhamnogalacturonanarabinogalactanprebiotic<i>Lactobacillus</i> |
| spellingShingle | Arland T. Hotchkiss John A. Renye Andre K. White Alberto Nunez Giselle K. P. Guron Hoa Chau Stefanie Simon Carlos Poveda Gemma Walton Robert Rastall Christina Khoo Cranberry Arabino-Xyloglucan and Pectic Oligosaccharides Induce <i>Lactobacillus</i> Growth and Short-Chain Fatty Acid Production Microorganisms cranberry xyloglucan rhamnogalacturonan arabinogalactan prebiotic <i>Lactobacillus</i> |
| title | Cranberry Arabino-Xyloglucan and Pectic Oligosaccharides Induce <i>Lactobacillus</i> Growth and Short-Chain Fatty Acid Production |
| title_full | Cranberry Arabino-Xyloglucan and Pectic Oligosaccharides Induce <i>Lactobacillus</i> Growth and Short-Chain Fatty Acid Production |
| title_fullStr | Cranberry Arabino-Xyloglucan and Pectic Oligosaccharides Induce <i>Lactobacillus</i> Growth and Short-Chain Fatty Acid Production |
| title_full_unstemmed | Cranberry Arabino-Xyloglucan and Pectic Oligosaccharides Induce <i>Lactobacillus</i> Growth and Short-Chain Fatty Acid Production |
| title_short | Cranberry Arabino-Xyloglucan and Pectic Oligosaccharides Induce <i>Lactobacillus</i> Growth and Short-Chain Fatty Acid Production |
| title_sort | cranberry arabino xyloglucan and pectic oligosaccharides induce i lactobacillus i growth and short chain fatty acid production |
| topic | cranberry xyloglucan rhamnogalacturonan arabinogalactan prebiotic <i>Lactobacillus</i> |
| url | https://www.mdpi.com/2076-2607/10/7/1346 |
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