Novel QTL for Lateral Root Density and Length Improve Phosphorus Uptake in Rice (Oryza sativa L.)
Abstract The rice root system consists of two types of lateral roots, indeterminate larger L-types capable of further branching, and determinate, short, unbranched S-types. L-type laterals correspond to the typical lateral roots of cereals whereas S-type laterals are unique to rice. Both types contr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-08-01
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| Series: | Rice |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12284-023-00654-z |
| _version_ | 1797556474799456256 |
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| author | Lam Thi Dinh Yoshiaki Ueda Daniel Gonzalez Juan Pariasca Tanaka Hideki Takanashi Matthias Wissuwa |
| author_facet | Lam Thi Dinh Yoshiaki Ueda Daniel Gonzalez Juan Pariasca Tanaka Hideki Takanashi Matthias Wissuwa |
| author_sort | Lam Thi Dinh |
| collection | DOAJ |
| description | Abstract The rice root system consists of two types of lateral roots, indeterminate larger L-types capable of further branching, and determinate, short, unbranched S-types. L-type laterals correspond to the typical lateral roots of cereals whereas S-type laterals are unique to rice. Both types contribute to nutrient and water uptake and genotypic variation for density and length of these laterals could be exploited in rice improvement to enhance adaptations to nutrient and water-limited environments. Our objectives were to determine how best to screen for lateral root density and length and to identify markers linked to genotypic variation for these traits. Using different growing media showed that screening in nutrient solution exposed genotypic variation for S-type and L-type density, but only the lateral roots of soil-grown plants varied for their lengths. A QTL mapping population developed from parents contrasting for lateral root traits was grown in a low-P field, roots were sampled, scanned and density and length of lateral roots measured. One QTL each was detected for L-type density (LDC), S-type density on crown root (SDC), S-type density on L-type (SDL), S-type length on L-type (SLL), and crown root number (RNO). The QTL for LDC on chromosome 5 had a major effect, accounting for 46% of the phenotypic variation. This strong positive effect was confirmed in additional field experiments, showing that lines with the donor parent allele at qLDC5 had 50% higher LDC. Investigating the contribution of lateral root traits to P uptake using stepwise regressions indicated LDC and RNO were most influential, followed by SDL. Simulating effects of root trait differences conferred by the main QTL in a P uptake model confirmed that qLDC5 was most effective in improving P uptake followed by qRNO9 for RNO and qSDL9 for S-type lateral density on L-type laterals. Pyramiding qLDC5 with qRNO9 and qSDL9 would be possible given that trade-offs between traits were not detected. Phenotypic selection for the RNO trait during variety development would be feasible, however, the costs of doing so reliably for lateral root density traits is prohibitive and markers identified here therefore provide the first opportunity to incorporate such traits into a breeding program. |
| first_indexed | 2024-03-10T17:02:23Z |
| format | Article |
| id | doaj.art-ea1ea0e240f04ec48294eaeb1a662e5d |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-03-10T17:02:23Z |
| publishDate | 2023-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-ea1ea0e240f04ec48294eaeb1a662e5d2023-11-20T10:55:55ZengSpringerOpenRice1939-84251939-84332023-08-0116111510.1186/s12284-023-00654-zNovel QTL for Lateral Root Density and Length Improve Phosphorus Uptake in Rice (Oryza sativa L.)Lam Thi Dinh0Yoshiaki Ueda1Daniel Gonzalez2Juan Pariasca Tanaka3Hideki Takanashi4Matthias Wissuwa5Crop, Livestock and Environment Division, Japan International Research Center for Agricultural Sciences (JIRCAS)Crop, Livestock and Environment Division, Japan International Research Center for Agricultural Sciences (JIRCAS)Crop, Livestock and Environment Division, Japan International Research Center for Agricultural Sciences (JIRCAS)Crop, Livestock and Environment Division, Japan International Research Center for Agricultural Sciences (JIRCAS)Graduate School of Agricultural and Life Sciences, The University of TokyoCrop, Livestock and Environment Division, Japan International Research Center for Agricultural Sciences (JIRCAS)Abstract The rice root system consists of two types of lateral roots, indeterminate larger L-types capable of further branching, and determinate, short, unbranched S-types. L-type laterals correspond to the typical lateral roots of cereals whereas S-type laterals are unique to rice. Both types contribute to nutrient and water uptake and genotypic variation for density and length of these laterals could be exploited in rice improvement to enhance adaptations to nutrient and water-limited environments. Our objectives were to determine how best to screen for lateral root density and length and to identify markers linked to genotypic variation for these traits. Using different growing media showed that screening in nutrient solution exposed genotypic variation for S-type and L-type density, but only the lateral roots of soil-grown plants varied for their lengths. A QTL mapping population developed from parents contrasting for lateral root traits was grown in a low-P field, roots were sampled, scanned and density and length of lateral roots measured. One QTL each was detected for L-type density (LDC), S-type density on crown root (SDC), S-type density on L-type (SDL), S-type length on L-type (SLL), and crown root number (RNO). The QTL for LDC on chromosome 5 had a major effect, accounting for 46% of the phenotypic variation. This strong positive effect was confirmed in additional field experiments, showing that lines with the donor parent allele at qLDC5 had 50% higher LDC. Investigating the contribution of lateral root traits to P uptake using stepwise regressions indicated LDC and RNO were most influential, followed by SDL. Simulating effects of root trait differences conferred by the main QTL in a P uptake model confirmed that qLDC5 was most effective in improving P uptake followed by qRNO9 for RNO and qSDL9 for S-type lateral density on L-type laterals. Pyramiding qLDC5 with qRNO9 and qSDL9 would be possible given that trade-offs between traits were not detected. Phenotypic selection for the RNO trait during variety development would be feasible, however, the costs of doing so reliably for lateral root density traits is prohibitive and markers identified here therefore provide the first opportunity to incorporate such traits into a breeding program.https://doi.org/10.1186/s12284-023-00654-zL-type lateral rootsS-type lateral rootsCrown rootP uptake simulationRoot system architecture |
| spellingShingle | Lam Thi Dinh Yoshiaki Ueda Daniel Gonzalez Juan Pariasca Tanaka Hideki Takanashi Matthias Wissuwa Novel QTL for Lateral Root Density and Length Improve Phosphorus Uptake in Rice (Oryza sativa L.) Rice L-type lateral roots S-type lateral roots Crown root P uptake simulation Root system architecture |
| title | Novel QTL for Lateral Root Density and Length Improve Phosphorus Uptake in Rice (Oryza sativa L.) |
| title_full | Novel QTL for Lateral Root Density and Length Improve Phosphorus Uptake in Rice (Oryza sativa L.) |
| title_fullStr | Novel QTL for Lateral Root Density and Length Improve Phosphorus Uptake in Rice (Oryza sativa L.) |
| title_full_unstemmed | Novel QTL for Lateral Root Density and Length Improve Phosphorus Uptake in Rice (Oryza sativa L.) |
| title_short | Novel QTL for Lateral Root Density and Length Improve Phosphorus Uptake in Rice (Oryza sativa L.) |
| title_sort | novel qtl for lateral root density and length improve phosphorus uptake in rice oryza sativa l |
| topic | L-type lateral roots S-type lateral roots Crown root P uptake simulation Root system architecture |
| url | https://doi.org/10.1186/s12284-023-00654-z |
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