Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial Activity
Cefotaxime (CTX) is a third-generation cephalosporin antibiotic with broad-spectrum activity against Gram negative and Gram positive bacteria. However, like other third-generation cephalosporin antibiotics, its efficacy is declining due to the increased prevalence of multidrug-resistant (MDR) pathog...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Polymers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4360/14/4/771 |
| _version_ | 1797476985911377920 |
|---|---|
| author | Turki Al Hagbani Syed Mohd Danish Rizvi Talib Hussain Khalid Mehmood Zeeshan Rafi Afrasim Moin Amr Selim Abu Lila Farhan Alshammari El-Sayed Khafagy Mohamed Rahamathulla Marwa H. Abdallah |
| author_facet | Turki Al Hagbani Syed Mohd Danish Rizvi Talib Hussain Khalid Mehmood Zeeshan Rafi Afrasim Moin Amr Selim Abu Lila Farhan Alshammari El-Sayed Khafagy Mohamed Rahamathulla Marwa H. Abdallah |
| author_sort | Turki Al Hagbani |
| collection | DOAJ |
| description | Cefotaxime (CTX) is a third-generation cephalosporin antibiotic with broad-spectrum activity against Gram negative and Gram positive bacteria. However, like other third-generation cephalosporin antibiotics, its efficacy is declining due to the increased prevalence of multidrug-resistant (MDR) pathogens. Recent advances in nanotechnology have been projected as a practical approach to combat MDR microorganisms. Therefore, in the current study, gold nanoparticles (AuNPs) were prepared using cefotaxime sodium, which acted as a reducing and capping agent, besides having well-established antibacterial activity. The synthesized cefotaxime-loaded gold nanoparticles (C-AuNPs) were characterized by UV-Visible spectroscopy, FTIR, TEM and DLS. In addition, the in vitro antibacterial activity of C-AuNPs was assessed against both Gram-positive and Gram-negative bacteria. UV-Visible spectroscopy verified the formation of C-AuNPs, while TEM and DLS verified their nano-size. In addition, CTX loading onto AuNPs was confirmed by FTIR. Furthermore, the colloidal stability of the synthesized C-AuNPs was ascribed to the higher net negative surface charge of C-AuNPs. Most importantly, the synthesized C-AuNPs showed superior antibacterial activity and lower minimum inhibitory concentration (MIC) values against Gram-negative (<i>Escherichia coli</i>, <i>Klebsiella oxytoca</i>, <i>Pseudomonas aeruginosa</i>) and gram-positive (<i>Staphylococcus aureus</i>) bacteria, compared with pure CTX. Collectively, CTX was successfully adopted, as reducing and capping agent, to synthesize stable, nano-sized spherical C-AuNPs. Furthermore, loading CTX onto AuNPs could efficiently restore and/or boost the antibacterial activity of CTX against resistant Gram-negative and Gram-positive bacteria. |
| first_indexed | 2024-03-09T21:11:34Z |
| format | Article |
| id | doaj.art-ea221dd453af4ff79215081482ac32a9 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T21:11:34Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-ea221dd453af4ff79215081482ac32a92023-11-23T21:45:39ZengMDPI AGPolymers2073-43602022-02-0114477110.3390/polym14040771Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial ActivityTurki Al Hagbani0Syed Mohd Danish Rizvi1Talib Hussain2Khalid Mehmood3Zeeshan Rafi4Afrasim Moin5Amr Selim Abu Lila6Farhan Alshammari7El-Sayed Khafagy8Mohamed Rahamathulla9Marwa H. Abdallah10Department of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmacy, Abbottabad University of Science and Technology, Havelian 22500, PakistanNanomedicine and Nanotechnology Lab-6 (IIRC), Department of Biosciences, Integral University Lucknow, Lucknow 226026, IndiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj 11942, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, King Khalid University (KKU), Abha 61421, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Ha’il, Ha’il 81442, Saudi ArabiaCefotaxime (CTX) is a third-generation cephalosporin antibiotic with broad-spectrum activity against Gram negative and Gram positive bacteria. However, like other third-generation cephalosporin antibiotics, its efficacy is declining due to the increased prevalence of multidrug-resistant (MDR) pathogens. Recent advances in nanotechnology have been projected as a practical approach to combat MDR microorganisms. Therefore, in the current study, gold nanoparticles (AuNPs) were prepared using cefotaxime sodium, which acted as a reducing and capping agent, besides having well-established antibacterial activity. The synthesized cefotaxime-loaded gold nanoparticles (C-AuNPs) were characterized by UV-Visible spectroscopy, FTIR, TEM and DLS. In addition, the in vitro antibacterial activity of C-AuNPs was assessed against both Gram-positive and Gram-negative bacteria. UV-Visible spectroscopy verified the formation of C-AuNPs, while TEM and DLS verified their nano-size. In addition, CTX loading onto AuNPs was confirmed by FTIR. Furthermore, the colloidal stability of the synthesized C-AuNPs was ascribed to the higher net negative surface charge of C-AuNPs. Most importantly, the synthesized C-AuNPs showed superior antibacterial activity and lower minimum inhibitory concentration (MIC) values against Gram-negative (<i>Escherichia coli</i>, <i>Klebsiella oxytoca</i>, <i>Pseudomonas aeruginosa</i>) and gram-positive (<i>Staphylococcus aureus</i>) bacteria, compared with pure CTX. Collectively, CTX was successfully adopted, as reducing and capping agent, to synthesize stable, nano-sized spherical C-AuNPs. Furthermore, loading CTX onto AuNPs could efficiently restore and/or boost the antibacterial activity of CTX against resistant Gram-negative and Gram-positive bacteria.https://www.mdpi.com/2073-4360/14/4/771bacterial resistancecefotaxime sodiumcephalosporingold nanoparticlesMIC<sub>50</sub> |
| spellingShingle | Turki Al Hagbani Syed Mohd Danish Rizvi Talib Hussain Khalid Mehmood Zeeshan Rafi Afrasim Moin Amr Selim Abu Lila Farhan Alshammari El-Sayed Khafagy Mohamed Rahamathulla Marwa H. Abdallah Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial Activity Polymers bacterial resistance cefotaxime sodium cephalosporin gold nanoparticles MIC<sub>50</sub> |
| title | Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial Activity |
| title_full | Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial Activity |
| title_fullStr | Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial Activity |
| title_full_unstemmed | Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial Activity |
| title_short | Cefotaxime Mediated Synthesis of Gold Nanoparticles: Characterization and Antibacterial Activity |
| title_sort | cefotaxime mediated synthesis of gold nanoparticles characterization and antibacterial activity |
| topic | bacterial resistance cefotaxime sodium cephalosporin gold nanoparticles MIC<sub>50</sub> |
| url | https://www.mdpi.com/2073-4360/14/4/771 |
| work_keys_str_mv | AT turkialhagbani cefotaximemediatedsynthesisofgoldnanoparticlescharacterizationandantibacterialactivity AT syedmohddanishrizvi cefotaximemediatedsynthesisofgoldnanoparticlescharacterizationandantibacterialactivity AT talibhussain cefotaximemediatedsynthesisofgoldnanoparticlescharacterizationandantibacterialactivity AT khalidmehmood cefotaximemediatedsynthesisofgoldnanoparticlescharacterizationandantibacterialactivity AT zeeshanrafi cefotaximemediatedsynthesisofgoldnanoparticlescharacterizationandantibacterialactivity AT afrasimmoin cefotaximemediatedsynthesisofgoldnanoparticlescharacterizationandantibacterialactivity AT amrselimabulila cefotaximemediatedsynthesisofgoldnanoparticlescharacterizationandantibacterialactivity AT farhanalshammari cefotaximemediatedsynthesisofgoldnanoparticlescharacterizationandantibacterialactivity AT elsayedkhafagy cefotaximemediatedsynthesisofgoldnanoparticlescharacterizationandantibacterialactivity AT mohamedrahamathulla cefotaximemediatedsynthesisofgoldnanoparticlescharacterizationandantibacterialactivity AT marwahabdallah cefotaximemediatedsynthesisofgoldnanoparticlescharacterizationandantibacterialactivity |