Mitochondrial Markers for the Detection of Duck Breeds Using Polymerase Chain Reaction
Species identification of the components of various duck breeds has revealed that the lowest identifiable number of components depends on the breed. The results (shown on the agarose gel) of a species-specific PCR reaction for Rouen ducks were less intense than the results for the same amount of com...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/6/857 |
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| author | Małgorzata Natonek-Wiśniewska Piotr Krzyścin Dominika Rubiś |
| author_facet | Małgorzata Natonek-Wiśniewska Piotr Krzyścin Dominika Rubiś |
| author_sort | Małgorzata Natonek-Wiśniewska |
| collection | DOAJ |
| description | Species identification of the components of various duck breeds has revealed that the lowest identifiable number of components depends on the breed. The results (shown on the agarose gel) of a species-specific PCR reaction for Rouen ducks were less intense than the results for the same amount of components from other popular duck breeds, suggesting differences in the Rouen duck genome. Therefore, the present study aimed to identify part of the Rouen duck’s gene sequences and to develop two new primer pairs. The first pair enables breed-independent identification of duck DNA, and the second distinguishes Rouen ducks from Chinese and Indian Runner ducks. The sequencing reaction yielded sequences of 1386 bp in length, and the identified sequence differs by around 7% from the sequences of Chinese duck species. The detected sequence contributes to improving species identification methods for duck DNA. On its basis, two primers for the identification of duck DNA were designed. The first allows for DNA amplification with the same sensitivity regardless of duck breed. The second primer’s pair is breed specific, and it distinguishes Rouen ducks from Chinese and Indian Runner ducks. Both methods are very sensitive (0.05%). |
| first_indexed | 2024-03-10T10:45:22Z |
| format | Article |
| id | doaj.art-ea2287f726424d46a321b8c2d12e767d |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T10:45:22Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-ea2287f726424d46a321b8c2d12e767d2023-11-21T22:38:35ZengMDPI AGGenes2073-44252021-06-0112685710.3390/genes12060857Mitochondrial Markers for the Detection of Duck Breeds Using Polymerase Chain ReactionMałgorzata Natonek-Wiśniewska0Piotr Krzyścin1Dominika Rubiś2Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandDepartment of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandDepartment of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandSpecies identification of the components of various duck breeds has revealed that the lowest identifiable number of components depends on the breed. The results (shown on the agarose gel) of a species-specific PCR reaction for Rouen ducks were less intense than the results for the same amount of components from other popular duck breeds, suggesting differences in the Rouen duck genome. Therefore, the present study aimed to identify part of the Rouen duck’s gene sequences and to develop two new primer pairs. The first pair enables breed-independent identification of duck DNA, and the second distinguishes Rouen ducks from Chinese and Indian Runner ducks. The sequencing reaction yielded sequences of 1386 bp in length, and the identified sequence differs by around 7% from the sequences of Chinese duck species. The detected sequence contributes to improving species identification methods for duck DNA. On its basis, two primers for the identification of duck DNA were designed. The first allows for DNA amplification with the same sensitivity regardless of duck breed. The second primer’s pair is breed specific, and it distinguishes Rouen ducks from Chinese and Indian Runner ducks. Both methods are very sensitive (0.05%).https://www.mdpi.com/2073-4425/12/6/85712S rRNA16S rRNARouenMH078252identification of duck DNAmtDNA of duck |
| spellingShingle | Małgorzata Natonek-Wiśniewska Piotr Krzyścin Dominika Rubiś Mitochondrial Markers for the Detection of Duck Breeds Using Polymerase Chain Reaction Genes 12S rRNA 16S rRNA Rouen MH078252 identification of duck DNA mtDNA of duck |
| title | Mitochondrial Markers for the Detection of Duck Breeds Using Polymerase Chain Reaction |
| title_full | Mitochondrial Markers for the Detection of Duck Breeds Using Polymerase Chain Reaction |
| title_fullStr | Mitochondrial Markers for the Detection of Duck Breeds Using Polymerase Chain Reaction |
| title_full_unstemmed | Mitochondrial Markers for the Detection of Duck Breeds Using Polymerase Chain Reaction |
| title_short | Mitochondrial Markers for the Detection of Duck Breeds Using Polymerase Chain Reaction |
| title_sort | mitochondrial markers for the detection of duck breeds using polymerase chain reaction |
| topic | 12S rRNA 16S rRNA Rouen MH078252 identification of duck DNA mtDNA of duck |
| url | https://www.mdpi.com/2073-4425/12/6/857 |
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