A high performance thin layer chromatographic method for the estimation of colchicine in different formulations
Background: Colchicine is a main alkaloid present in bitter and sweet variety of colchicum (Colchicum luteum Baker), which have been reported to possess anti-rheumatic, anti-gout, and anticancer potential. Colchicum is an important ingredient of several Unani and Herbal formulations. Quantification...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2015;volume=7;issue=4;spage=260;epage=263;aulast=Fahim |
| _version_ | 1818557101271678976 |
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| author | Mohd Fahim Mhaveer Singh Y T Kamal Hayat M Mukhtar Sayeed Ahmad |
| author_facet | Mohd Fahim Mhaveer Singh Y T Kamal Hayat M Mukhtar Sayeed Ahmad |
| author_sort | Mohd Fahim |
| collection | DOAJ |
| description | Background: Colchicine is a main alkaloid present in bitter and sweet variety of colchicum (Colchicum luteum Baker), which have been reported to possess anti-rheumatic, anti-gout, and anticancer potential. Colchicum is an important ingredient of several Unani and Herbal formulations. Quantification of colchicine will play a great role in quality control of these formulations. Hence, a high-performance thin layer chromatographic (TLC) method has been developed for the analysis of colchicine in Unani formulations of various dosage forms such as hubb (tablet) and capsules. Materials and Methods: The samples were applied on aluminum TLC plates precoated with silica gel 60-F254and developed using mobile phase toluene-dichloromethane-methanol in equal proportions. Quantification was done by densitometric scanning at 350 nm, which showed a linear response in the range of 50–500 ng/spot. The developed method was validated as per the International Conference on Harmonization guidelines for linearity, precision, accuracy, specificity, robustness, limit of detection, and limit of quantification. Results and Conclusion: The developed method was applied for quantitative estimation of colchicine in different Unani and Herbal formulations. The method was found simple, selective, accurate with a wide range of linearity, hence suitable for the quality control of different formulations and varieties of colchicum with respect to colchicine content. |
| first_indexed | 2024-12-13T23:55:36Z |
| format | Article |
| id | doaj.art-ea2a7f1695274508b3aafaa392d7479a |
| institution | Directory Open Access Journal |
| issn | 0975-7406 0976-4879 |
| language | English |
| last_indexed | 2024-12-13T23:55:36Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj.art-ea2a7f1695274508b3aafaa392d7479a2022-12-21T23:26:34ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0975-74060976-48792015-01-017426026310.4103/0975-7406.168021A high performance thin layer chromatographic method for the estimation of colchicine in different formulationsMohd FahimMhaveer SinghY T KamalHayat M MukhtarSayeed AhmadBackground: Colchicine is a main alkaloid present in bitter and sweet variety of colchicum (Colchicum luteum Baker), which have been reported to possess anti-rheumatic, anti-gout, and anticancer potential. Colchicum is an important ingredient of several Unani and Herbal formulations. Quantification of colchicine will play a great role in quality control of these formulations. Hence, a high-performance thin layer chromatographic (TLC) method has been developed for the analysis of colchicine in Unani formulations of various dosage forms such as hubb (tablet) and capsules. Materials and Methods: The samples were applied on aluminum TLC plates precoated with silica gel 60-F254and developed using mobile phase toluene-dichloromethane-methanol in equal proportions. Quantification was done by densitometric scanning at 350 nm, which showed a linear response in the range of 50–500 ng/spot. The developed method was validated as per the International Conference on Harmonization guidelines for linearity, precision, accuracy, specificity, robustness, limit of detection, and limit of quantification. Results and Conclusion: The developed method was applied for quantitative estimation of colchicine in different Unani and Herbal formulations. The method was found simple, selective, accurate with a wide range of linearity, hence suitable for the quality control of different formulations and varieties of colchicum with respect to colchicine content.http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2015;volume=7;issue=4;spage=260;epage=263;aulast=FahimColchicineColchicum luteumestimationhigh-performance thin layer chromatographicvalidation |
| spellingShingle | Mohd Fahim Mhaveer Singh Y T Kamal Hayat M Mukhtar Sayeed Ahmad A high performance thin layer chromatographic method for the estimation of colchicine in different formulations Journal of Pharmacy and Bioallied Sciences Colchicine Colchicum luteum estimation high-performance thin layer chromatographic validation |
| title | A high performance thin layer chromatographic method for the estimation of colchicine in different formulations |
| title_full | A high performance thin layer chromatographic method for the estimation of colchicine in different formulations |
| title_fullStr | A high performance thin layer chromatographic method for the estimation of colchicine in different formulations |
| title_full_unstemmed | A high performance thin layer chromatographic method for the estimation of colchicine in different formulations |
| title_short | A high performance thin layer chromatographic method for the estimation of colchicine in different formulations |
| title_sort | high performance thin layer chromatographic method for the estimation of colchicine in different formulations |
| topic | Colchicine Colchicum luteum estimation high-performance thin layer chromatographic validation |
| url | http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2015;volume=7;issue=4;spage=260;epage=263;aulast=Fahim |
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