Transcriptomics Profiling of <i>Acer pseudosieboldianum</i> Molecular Mechanism against Freezing Stress
Low temperature is an important environmental factor that affects the growth and development of trees and leads to the introduction of failure in the genetic improvement of trees. <i>Acer pseudosieboldianum</i> is a tree species that is well-known for its bright red autumn leaf color. Th...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| author | Zhiming Han Xiangzhu Xu Shikai Zhang Qiushuang Zhao Hanxi Li Ying Cui Xiao Li Liran Wang Su Chen Xiyang Zhao |
| author_facet | Zhiming Han Xiangzhu Xu Shikai Zhang Qiushuang Zhao Hanxi Li Ying Cui Xiao Li Liran Wang Su Chen Xiyang Zhao |
| author_sort | Zhiming Han |
| collection | DOAJ |
| description | Low temperature is an important environmental factor that affects the growth and development of trees and leads to the introduction of failure in the genetic improvement of trees. <i>Acer pseudosieboldianum</i> is a tree species that is well-known for its bright red autumn leaf color. These trees are widely used in landscaping in northeast China. However, due to their poor cold resistance, introduced <i>A. pseudosieboldianum</i> trees suffer severe freezing injury in many introduced environments. To elucidate the physiological indicators and molecular mechanisms associated with freezing damage, we analyzed the physiological indicators and transcriptome of <i>A. pseudosieboldianum</i>, using kits and RNA-Seq technology. The mechanism of <i>A. pseudosieboldianum</i> in response to freezing stress is an important scientific question. In this study, we used the shoots of four-year-old <i>A. pseudosieboldianum</i> twig seedlings, and the physiological index and the transcriptome of <i>A. pseudosieboldianum</i> under low temperature stress were investigated. The results showed that more than 20,000 genes were detected in <i>A. pseudosieboldianum</i> under low temperature (4 °C) and freezing temperatures (−10 °C, −20 °C, −30 °C, and −40 °C). There were 2505, 6021, 5125, and 3191 differential genes (DEGs) between −10 °C, −20°C, −30°C, −40 °C, and CK (4 °C), respectively. Among these differential genes, 48 genes are involved in the MAPK pathway and 533 genes are involved in the glucose metabolism pathway. In addition, the important transcription factors (MYB, AP2/ERF, and WRKY) involved in freezing stress were activated under different degrees of freezing stress. A total of 10 sets of physiological indicators of <i>A. pseudosieboldianum</i> were examined, including the activities of five enzymes and the accumulation of five hormones. All of the physiological indicators except SOD and GSH-Px reached their maximum values at −30 °C. The enzyme activity of SOD was highest at −10 °C, and that of GSH-Px was highest at −20 °C. Our study is the first to provide a more comprehensive understanding of the differential genes (DEGs) involved in <i>A. pseudosieboldianum</i> under freezing stress at different temperatures at the transcriptome level. These results may help to clarify the molecular mechanism of cold tolerance of <i>A. pseudosieboldianum</i> and provide new insights and candidate genes for the genetic improvement of the freezing tolerance of <i>A. pseudosieboldianum</i>. |
| first_indexed | 2024-03-09T17:46:57Z |
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| spelling | doaj.art-ea2b91b5b5664d16ba02db63247ec89a2023-11-24T11:05:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231467610.3390/ijms232314676Transcriptomics Profiling of <i>Acer pseudosieboldianum</i> Molecular Mechanism against Freezing StressZhiming Han0Xiangzhu Xu1Shikai Zhang2Qiushuang Zhao3Hanxi Li4Ying Cui5Xiao Li6Liran Wang7Su Chen8Xiyang Zhao9State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaCollege of Veterinary Medicine, Jilin University, Changchun 130062, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaJilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural University, Changchun 130118, ChinaJilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural University, Changchun 130118, ChinaJilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural University, Changchun 130118, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaJilin Provincial Key Laboratory of Tree and Grass Genetics and Breeding, College of Forestry and Grassland Science, Jilin Agricultural University, Changchun 130118, ChinaLow temperature is an important environmental factor that affects the growth and development of trees and leads to the introduction of failure in the genetic improvement of trees. <i>Acer pseudosieboldianum</i> is a tree species that is well-known for its bright red autumn leaf color. These trees are widely used in landscaping in northeast China. However, due to their poor cold resistance, introduced <i>A. pseudosieboldianum</i> trees suffer severe freezing injury in many introduced environments. To elucidate the physiological indicators and molecular mechanisms associated with freezing damage, we analyzed the physiological indicators and transcriptome of <i>A. pseudosieboldianum</i>, using kits and RNA-Seq technology. The mechanism of <i>A. pseudosieboldianum</i> in response to freezing stress is an important scientific question. In this study, we used the shoots of four-year-old <i>A. pseudosieboldianum</i> twig seedlings, and the physiological index and the transcriptome of <i>A. pseudosieboldianum</i> under low temperature stress were investigated. The results showed that more than 20,000 genes were detected in <i>A. pseudosieboldianum</i> under low temperature (4 °C) and freezing temperatures (−10 °C, −20 °C, −30 °C, and −40 °C). There were 2505, 6021, 5125, and 3191 differential genes (DEGs) between −10 °C, −20°C, −30°C, −40 °C, and CK (4 °C), respectively. Among these differential genes, 48 genes are involved in the MAPK pathway and 533 genes are involved in the glucose metabolism pathway. In addition, the important transcription factors (MYB, AP2/ERF, and WRKY) involved in freezing stress were activated under different degrees of freezing stress. A total of 10 sets of physiological indicators of <i>A. pseudosieboldianum</i> were examined, including the activities of five enzymes and the accumulation of five hormones. All of the physiological indicators except SOD and GSH-Px reached their maximum values at −30 °C. The enzyme activity of SOD was highest at −10 °C, and that of GSH-Px was highest at −20 °C. Our study is the first to provide a more comprehensive understanding of the differential genes (DEGs) involved in <i>A. pseudosieboldianum</i> under freezing stress at different temperatures at the transcriptome level. These results may help to clarify the molecular mechanism of cold tolerance of <i>A. pseudosieboldianum</i> and provide new insights and candidate genes for the genetic improvement of the freezing tolerance of <i>A. pseudosieboldianum</i>.https://www.mdpi.com/1422-0067/23/23/14676<i>Acer pseudosieboldianum</i>freezing stressRNA-seqDEGs |
| spellingShingle | Zhiming Han Xiangzhu Xu Shikai Zhang Qiushuang Zhao Hanxi Li Ying Cui Xiao Li Liran Wang Su Chen Xiyang Zhao Transcriptomics Profiling of <i>Acer pseudosieboldianum</i> Molecular Mechanism against Freezing Stress International Journal of Molecular Sciences <i>Acer pseudosieboldianum</i> freezing stress RNA-seq DEGs |
| title | Transcriptomics Profiling of <i>Acer pseudosieboldianum</i> Molecular Mechanism against Freezing Stress |
| title_full | Transcriptomics Profiling of <i>Acer pseudosieboldianum</i> Molecular Mechanism against Freezing Stress |
| title_fullStr | Transcriptomics Profiling of <i>Acer pseudosieboldianum</i> Molecular Mechanism against Freezing Stress |
| title_full_unstemmed | Transcriptomics Profiling of <i>Acer pseudosieboldianum</i> Molecular Mechanism against Freezing Stress |
| title_short | Transcriptomics Profiling of <i>Acer pseudosieboldianum</i> Molecular Mechanism against Freezing Stress |
| title_sort | transcriptomics profiling of i acer pseudosieboldianum i molecular mechanism against freezing stress |
| topic | <i>Acer pseudosieboldianum</i> freezing stress RNA-seq DEGs |
| url | https://www.mdpi.com/1422-0067/23/23/14676 |
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