Malacological survey in a bottle of water: A comparative study between manual sampling and environmental DNA metabarcoding approaches
To assess the effect of anthropogenic activities on ecosystems, it is of prime importance to develop new tools enabling a rapid characterization of ecological communities. Freshwater ecosystems are particularly impacted and threatened by human activities and need thorough attention to preserve their...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-01-01
|
| Series: | Global Ecology and Conservation |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2351989420309707 |
| _version_ | 1818920229448712192 |
|---|---|
| author | Stephen Mulero Eve Toulza Anaïs Loisier Meryl Zimmerman Jean-François Allienne Joséphine Foata Yann Quilichini Jean-Pierre Pointier Olivier Rey Jérôme Boissier |
| author_facet | Stephen Mulero Eve Toulza Anaïs Loisier Meryl Zimmerman Jean-François Allienne Joséphine Foata Yann Quilichini Jean-Pierre Pointier Olivier Rey Jérôme Boissier |
| author_sort | Stephen Mulero |
| collection | DOAJ |
| description | To assess the effect of anthropogenic activities on ecosystems, it is of prime importance to develop new tools enabling a rapid characterization of ecological communities. Freshwater ecosystems are particularly impacted and threatened by human activities and need thorough attention to preserve their biodiversity and the ecological services they provide. Studying such ecosystems is generally difficult because the associated organisms are hard to sample and to monitor. We present a ready-to-use environmental metabarcoding protocol to characterize and monitor the freshwater gastropods communities from water samples. The efficiency of this new tool was compared to a classical malacological survey at 19 sampled sites from 10 distinct rivers distributed over Corsica Island (France). From a single water sample, our eDNA monitoring tool provided a faithful characterization of the local malacofauna compared to the results obtained from the classical malacological survey, with 97.1% of species detection confirmed by both methods. The present tool successfully detected the 11 freshwater snail species previously reported in Corsica by malacological survey but was limited at the genus level for some species. Moreover, our malacological survey allowed an update of the local distribution of a wide diversity of freshwater snails including invasive species (i.e. Potamopyrgus antipodarum and Physa acuta) as well as snail hosts of pathogens of medical and veterinary importance (i.e. Bulinus truncatus and Galba truncatula). These results strengthened a previous hypothesis of an eventual competitive interaction between B. truncatus and P. antipodarum that could limit the endemization of the uro-genital bilharziasis in Corsica. |
| first_indexed | 2024-12-20T01:18:26Z |
| format | Article |
| id | doaj.art-ea2b977aad4a40d6a3ff3eeb62824b10 |
| institution | Directory Open Access Journal |
| issn | 2351-9894 |
| language | English |
| last_indexed | 2024-12-20T01:18:26Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Global Ecology and Conservation |
| spelling | doaj.art-ea2b977aad4a40d6a3ff3eeb62824b102022-12-21T19:58:31ZengElsevierGlobal Ecology and Conservation2351-98942021-01-0125e01428Malacological survey in a bottle of water: A comparative study between manual sampling and environmental DNA metabarcoding approachesStephen Mulero0Eve Toulza1Anaïs Loisier2Meryl Zimmerman3Jean-François Allienne4Joséphine Foata5Yann Quilichini6Jean-Pierre Pointier7Olivier Rey8Jérôme Boissier9University of Perpignan, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, France; Corresponding author. Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, 66860, Perpignan cedex 9, France.University of Perpignan, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, FranceUniversity of Perpignan, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, FranceUniversity of Perpignan, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, FranceUniversity of Perpignan, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, FranceUMR SPE 6134, CNRS – Università di Corsica Pascal Paoli, 20250, Corte, Corsica, FranceUMR SPE 6134, CNRS – Università di Corsica Pascal Paoli, 20250, Corte, Corsica, FrancePSL University, USR3278 CRIOBE EPHE-CNRS-UPVD, F-66860, Perpignan, FranceUniversity of Perpignan, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, FranceUniversity of Perpignan, IHPE UMR 5244, CNRS, IFREMER, Univ. Montpellier, F-66860, Perpignan, FranceTo assess the effect of anthropogenic activities on ecosystems, it is of prime importance to develop new tools enabling a rapid characterization of ecological communities. Freshwater ecosystems are particularly impacted and threatened by human activities and need thorough attention to preserve their biodiversity and the ecological services they provide. Studying such ecosystems is generally difficult because the associated organisms are hard to sample and to monitor. We present a ready-to-use environmental metabarcoding protocol to characterize and monitor the freshwater gastropods communities from water samples. The efficiency of this new tool was compared to a classical malacological survey at 19 sampled sites from 10 distinct rivers distributed over Corsica Island (France). From a single water sample, our eDNA monitoring tool provided a faithful characterization of the local malacofauna compared to the results obtained from the classical malacological survey, with 97.1% of species detection confirmed by both methods. The present tool successfully detected the 11 freshwater snail species previously reported in Corsica by malacological survey but was limited at the genus level for some species. Moreover, our malacological survey allowed an update of the local distribution of a wide diversity of freshwater snails including invasive species (i.e. Potamopyrgus antipodarum and Physa acuta) as well as snail hosts of pathogens of medical and veterinary importance (i.e. Bulinus truncatus and Galba truncatula). These results strengthened a previous hypothesis of an eventual competitive interaction between B. truncatus and P. antipodarum that could limit the endemization of the uro-genital bilharziasis in Corsica.http://www.sciencedirect.com/science/article/pii/S2351989420309707BiomonitoringCorsicaEnvironmental DNAMalacologyMetabarcodingSnail communities |
| spellingShingle | Stephen Mulero Eve Toulza Anaïs Loisier Meryl Zimmerman Jean-François Allienne Joséphine Foata Yann Quilichini Jean-Pierre Pointier Olivier Rey Jérôme Boissier Malacological survey in a bottle of water: A comparative study between manual sampling and environmental DNA metabarcoding approaches Global Ecology and Conservation Biomonitoring Corsica Environmental DNA Malacology Metabarcoding Snail communities |
| title | Malacological survey in a bottle of water: A comparative study between manual sampling and environmental DNA metabarcoding approaches |
| title_full | Malacological survey in a bottle of water: A comparative study between manual sampling and environmental DNA metabarcoding approaches |
| title_fullStr | Malacological survey in a bottle of water: A comparative study between manual sampling and environmental DNA metabarcoding approaches |
| title_full_unstemmed | Malacological survey in a bottle of water: A comparative study between manual sampling and environmental DNA metabarcoding approaches |
| title_short | Malacological survey in a bottle of water: A comparative study between manual sampling and environmental DNA metabarcoding approaches |
| title_sort | malacological survey in a bottle of water a comparative study between manual sampling and environmental dna metabarcoding approaches |
| topic | Biomonitoring Corsica Environmental DNA Malacology Metabarcoding Snail communities |
| url | http://www.sciencedirect.com/science/article/pii/S2351989420309707 |
| work_keys_str_mv | AT stephenmulero malacologicalsurveyinabottleofwateracomparativestudybetweenmanualsamplingandenvironmentaldnametabarcodingapproaches AT evetoulza malacologicalsurveyinabottleofwateracomparativestudybetweenmanualsamplingandenvironmentaldnametabarcodingapproaches AT anaisloisier malacologicalsurveyinabottleofwateracomparativestudybetweenmanualsamplingandenvironmentaldnametabarcodingapproaches AT merylzimmerman malacologicalsurveyinabottleofwateracomparativestudybetweenmanualsamplingandenvironmentaldnametabarcodingapproaches AT jeanfrancoisallienne malacologicalsurveyinabottleofwateracomparativestudybetweenmanualsamplingandenvironmentaldnametabarcodingapproaches AT josephinefoata malacologicalsurveyinabottleofwateracomparativestudybetweenmanualsamplingandenvironmentaldnametabarcodingapproaches AT yannquilichini malacologicalsurveyinabottleofwateracomparativestudybetweenmanualsamplingandenvironmentaldnametabarcodingapproaches AT jeanpierrepointier malacologicalsurveyinabottleofwateracomparativestudybetweenmanualsamplingandenvironmentaldnametabarcodingapproaches AT olivierrey malacologicalsurveyinabottleofwateracomparativestudybetweenmanualsamplingandenvironmentaldnametabarcodingapproaches AT jeromeboissier malacologicalsurveyinabottleofwateracomparativestudybetweenmanualsamplingandenvironmentaldnametabarcodingapproaches |