A Method for Assessing the Robustness of Protein Structures by Randomizing Packing Interactions
Many single-domain proteins are not only stable and water-soluble, but they also populate few to no intermediates during folding. This reduces interactions between partially folded proteins, misfolding, and aggregation, and makes the proteins tractable in biotechnological applications. Natural prote...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2022-06-01
|
Series: | Frontiers in Molecular Biosciences |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fmolb.2022.849272/full |
_version_ | 1811238688686866432 |
---|---|
author | Shilpa Yadahalli Lakshmi P. Jayanthi Shachi Gosavi |
author_facet | Shilpa Yadahalli Lakshmi P. Jayanthi Shachi Gosavi |
author_sort | Shilpa Yadahalli |
collection | DOAJ |
description | Many single-domain proteins are not only stable and water-soluble, but they also populate few to no intermediates during folding. This reduces interactions between partially folded proteins, misfolding, and aggregation, and makes the proteins tractable in biotechnological applications. Natural proteins fold thus, not necessarily only because their structures are well-suited for folding, but because their sequences optimize packing and fit their structures well. In contrast, folding experiments on the de novo designed Top7 suggest that it populates several intermediates. Additionally, in de novo protein design, where sequences are designed for natural and new non-natural structures, tens of sequences still need to be tested before success is achieved. Both these issues may be caused by the specific scaffolds used in design, i.e., some protein scaffolds may be more tolerant to packing perturbations and varied sequences. Here, we report a computational method for assessing the response of protein structures to packing perturbations. We then benchmark this method using designed proteins and find that it can identify scaffolds whose folding gets disrupted upon perturbing packing, leading to the population of intermediates. The method can also isolate regions of both natural and designed scaffolds that are sensitive to such perturbations and identify contacts which when present can rescue folding. Overall, this method can be used to identify protein scaffolds that are more amenable to whole protein design as well as to identify protein regions which are sensitive to perturbations and where further mutations should be avoided during protein engineering. |
first_indexed | 2024-04-12T12:46:37Z |
format | Article |
id | doaj.art-ea2dc114c13440969612b7f7878c4126 |
institution | Directory Open Access Journal |
issn | 2296-889X |
language | English |
last_indexed | 2024-04-12T12:46:37Z |
publishDate | 2022-06-01 |
publisher | Frontiers Media S.A. |
record_format | Article |
series | Frontiers in Molecular Biosciences |
spelling | doaj.art-ea2dc114c13440969612b7f7878c41262022-12-22T03:32:36ZengFrontiers Media S.A.Frontiers in Molecular Biosciences2296-889X2022-06-01910.3389/fmolb.2022.849272849272A Method for Assessing the Robustness of Protein Structures by Randomizing Packing InteractionsShilpa YadahalliLakshmi P. JayanthiShachi GosaviMany single-domain proteins are not only stable and water-soluble, but they also populate few to no intermediates during folding. This reduces interactions between partially folded proteins, misfolding, and aggregation, and makes the proteins tractable in biotechnological applications. Natural proteins fold thus, not necessarily only because their structures are well-suited for folding, but because their sequences optimize packing and fit their structures well. In contrast, folding experiments on the de novo designed Top7 suggest that it populates several intermediates. Additionally, in de novo protein design, where sequences are designed for natural and new non-natural structures, tens of sequences still need to be tested before success is achieved. Both these issues may be caused by the specific scaffolds used in design, i.e., some protein scaffolds may be more tolerant to packing perturbations and varied sequences. Here, we report a computational method for assessing the response of protein structures to packing perturbations. We then benchmark this method using designed proteins and find that it can identify scaffolds whose folding gets disrupted upon perturbing packing, leading to the population of intermediates. The method can also isolate regions of both natural and designed scaffolds that are sensitive to such perturbations and identify contacts which when present can rescue folding. Overall, this method can be used to identify protein scaffolds that are more amenable to whole protein design as well as to identify protein regions which are sensitive to perturbations and where further mutations should be avoided during protein engineering.https://www.frontiersin.org/articles/10.3389/fmolb.2022.849272/fullpacking perturbationsprotein scaffoldstructure-based modelsmolecular dynamics simulationssequence permutationsrobustness of protein structure |
spellingShingle | Shilpa Yadahalli Lakshmi P. Jayanthi Shachi Gosavi A Method for Assessing the Robustness of Protein Structures by Randomizing Packing Interactions Frontiers in Molecular Biosciences packing perturbations protein scaffold structure-based models molecular dynamics simulations sequence permutations robustness of protein structure |
title | A Method for Assessing the Robustness of Protein Structures by Randomizing Packing Interactions |
title_full | A Method for Assessing the Robustness of Protein Structures by Randomizing Packing Interactions |
title_fullStr | A Method for Assessing the Robustness of Protein Structures by Randomizing Packing Interactions |
title_full_unstemmed | A Method for Assessing the Robustness of Protein Structures by Randomizing Packing Interactions |
title_short | A Method for Assessing the Robustness of Protein Structures by Randomizing Packing Interactions |
title_sort | method for assessing the robustness of protein structures by randomizing packing interactions |
topic | packing perturbations protein scaffold structure-based models molecular dynamics simulations sequence permutations robustness of protein structure |
url | https://www.frontiersin.org/articles/10.3389/fmolb.2022.849272/full |
work_keys_str_mv | AT shilpayadahalli amethodforassessingtherobustnessofproteinstructuresbyrandomizingpackinginteractions AT lakshmipjayanthi amethodforassessingtherobustnessofproteinstructuresbyrandomizingpackinginteractions AT shachigosavi amethodforassessingtherobustnessofproteinstructuresbyrandomizingpackinginteractions AT shilpayadahalli methodforassessingtherobustnessofproteinstructuresbyrandomizingpackinginteractions AT lakshmipjayanthi methodforassessingtherobustnessofproteinstructuresbyrandomizingpackinginteractions AT shachigosavi methodforassessingtherobustnessofproteinstructuresbyrandomizingpackinginteractions |