Fabrication of highly porous biodegradable biomimetic nanocomposite as advanced bone tissue scaffold
Development of bioinspired or biomimetic materials is currently a challenge in the field of tissue regeneration. In-situ 3D biomimetic microporous nanocomposite scaffold has been developed using a simple lyophilization post hydrothermal reaction for bone healing applications. The fabricated 3D porou...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-02-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535216301654 |
| _version_ | 1811331431987675136 |
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| author | Abdalla Abdal-hay Khalil Abdelrazek Khalil Abdel Salam Hamdy Fawzi F. Al-Jassir |
| author_facet | Abdalla Abdal-hay Khalil Abdelrazek Khalil Abdel Salam Hamdy Fawzi F. Al-Jassir |
| author_sort | Abdalla Abdal-hay |
| collection | DOAJ |
| description | Development of bioinspired or biomimetic materials is currently a challenge in the field of tissue regeneration. In-situ 3D biomimetic microporous nanocomposite scaffold has been developed using a simple lyophilization post hydrothermal reaction for bone healing applications. The fabricated 3D porous scaffold possesses advantages of good bonelike apatite particles distribution, thermal properties and high porous interconnected network structure. High dispersion bonelike apatite nanoparticles (NPs) rapidly nucleated and deposited from surrounding biological minerals within chitosan (CTS) matrices using hydrothermal technique. After that, freeze-drying method was applied on the composite solution to form the desired porous 3D architecture. Interestingly, the porosity and pore size of composite scaffold were not significantly affected by the particles size and particles content within the CTS matrix. Our results demonstrated that the compression modulus of porous composite scaffold is twice higher than that of plain CTS scaffold, indicating a maximization of the chemical interaction between polymer matrix and apatite NPs. Cytocompatibility test for MC3T3-E1 pre-osteoblasts cell line using MTT-indirect assay test showed that the fabricated 3D microporous nanocomposite scaffold possesses higher cell proliferation and growth than that of pure CTS scaffold. Collectively, our results suggest that the newly developed highly porous apatite/CTS nanocomposite scaffold as an alternative of hydroxyapatite/CTS scaffold may serve as an excellent porous 3D platform for bone tissue regeneration. |
| first_indexed | 2024-04-13T16:20:24Z |
| format | Article |
| id | doaj.art-ea2dd5b1bc0244669a70ac4063012405 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-13T16:20:24Z |
| publishDate | 2017-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-ea2dd5b1bc0244669a70ac40630124052022-12-22T02:39:57ZengElsevierArabian Journal of Chemistry1878-53522017-02-0110224025210.1016/j.arabjc.2016.09.021Fabrication of highly porous biodegradable biomimetic nanocomposite as advanced bone tissue scaffoldAbdalla Abdal-hay0Khalil Abdelrazek Khalil1Abdel Salam Hamdy2Fawzi F. Al-Jassir3Dept. of Engineering Materials and Mechanical Design, Faculty of Engineering, South Valley University, Qena, EgyptDepartment of Mechanical Engineering, College of Engineering, University of Sharjah, P.O. Box 27272, Sharjah, United Arab EmiratesDept. of Manufacturing and Industrial Engineering, College of Engineering and Computer Science, University of Texas Rio Grande Valley, 1201 West University Dr., Edinburg, TX 78541-2999, USAFRCSC, College of Medicine, King Saud University, Riyadh 11461 Saudi ArabiaDevelopment of bioinspired or biomimetic materials is currently a challenge in the field of tissue regeneration. In-situ 3D biomimetic microporous nanocomposite scaffold has been developed using a simple lyophilization post hydrothermal reaction for bone healing applications. The fabricated 3D porous scaffold possesses advantages of good bonelike apatite particles distribution, thermal properties and high porous interconnected network structure. High dispersion bonelike apatite nanoparticles (NPs) rapidly nucleated and deposited from surrounding biological minerals within chitosan (CTS) matrices using hydrothermal technique. After that, freeze-drying method was applied on the composite solution to form the desired porous 3D architecture. Interestingly, the porosity and pore size of composite scaffold were not significantly affected by the particles size and particles content within the CTS matrix. Our results demonstrated that the compression modulus of porous composite scaffold is twice higher than that of plain CTS scaffold, indicating a maximization of the chemical interaction between polymer matrix and apatite NPs. Cytocompatibility test for MC3T3-E1 pre-osteoblasts cell line using MTT-indirect assay test showed that the fabricated 3D microporous nanocomposite scaffold possesses higher cell proliferation and growth than that of pure CTS scaffold. Collectively, our results suggest that the newly developed highly porous apatite/CTS nanocomposite scaffold as an alternative of hydroxyapatite/CTS scaffold may serve as an excellent porous 3D platform for bone tissue regeneration.http://www.sciencedirect.com/science/article/pii/S1878535216301654Tissue engineeringNanocomposite scaffoldBioactive materialsBioinspired materials |
| spellingShingle | Abdalla Abdal-hay Khalil Abdelrazek Khalil Abdel Salam Hamdy Fawzi F. Al-Jassir Fabrication of highly porous biodegradable biomimetic nanocomposite as advanced bone tissue scaffold Arabian Journal of Chemistry Tissue engineering Nanocomposite scaffold Bioactive materials Bioinspired materials |
| title | Fabrication of highly porous biodegradable biomimetic nanocomposite as advanced bone tissue scaffold |
| title_full | Fabrication of highly porous biodegradable biomimetic nanocomposite as advanced bone tissue scaffold |
| title_fullStr | Fabrication of highly porous biodegradable biomimetic nanocomposite as advanced bone tissue scaffold |
| title_full_unstemmed | Fabrication of highly porous biodegradable biomimetic nanocomposite as advanced bone tissue scaffold |
| title_short | Fabrication of highly porous biodegradable biomimetic nanocomposite as advanced bone tissue scaffold |
| title_sort | fabrication of highly porous biodegradable biomimetic nanocomposite as advanced bone tissue scaffold |
| topic | Tissue engineering Nanocomposite scaffold Bioactive materials Bioinspired materials |
| url | http://www.sciencedirect.com/science/article/pii/S1878535216301654 |
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