Potent and Specific Antibacterial Activity against <i>Escherichia coli</i> O157:H7 and Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) of G17 and G19 Peptides Encapsulated into Poly-Lactic-Co-Glycolic Acid (PLGA) Nanoparticles
Antimicrobial peptides constitute an excellent alternative against conventional antibiotics because of their potent antimicrobial spectrum, unspecific action mechanism and low capacity to produce antibiotic resistance. However, a potential use of these biological molecules as therapeutic agents is t...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/7/384 |
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| author | Nicolás Gómez-Sequeda Jennifer Ruiz Claudia Ortiz Mauricio Urquiza Rodrigo Torres |
| author_facet | Nicolás Gómez-Sequeda Jennifer Ruiz Claudia Ortiz Mauricio Urquiza Rodrigo Torres |
| author_sort | Nicolás Gómez-Sequeda |
| collection | DOAJ |
| description | Antimicrobial peptides constitute an excellent alternative against conventional antibiotics because of their potent antimicrobial spectrum, unspecific action mechanism and low capacity to produce antibiotic resistance. However, a potential use of these biological molecules as therapeutic agents is threatened by their low stability and susceptibility to proteases. In order to overcome these limitations, encapsulation in biocompatible polymers as poly-lactic-glycolic-acid (PLGA) is a promising alternative for increasing their stability and bioavailability. In this work, the effect of new synthetic antimicrobial peptides GIBIM-P5S9K (G17) and GAM019 (G19) encapsulated on PLGA and acting against methicillin resistant <i>Staphylococus aureus</i> (MRSA) and <i>Escherichia coli</i> O157:H7 was studied. PLGA encapsulation allowed us to load around 7 µg AMPs/mg PLGA with an efficiency of 90.5%, capsule sizes around 290 nm and positive charges. Encapsulation improved antimicrobial activity, decreasing MIC50 from 1.5 to 0.2 (G17NP) and 0.7 (G19NP) µM against MRSA, and from 12.5 to 3.13 µM for <i>E. coli</i> O157:H7. Peptide loaded nanoparticles could be a bacteriostatic drug with potential application to treat these bacterial <i>E. coli</i> O157:H7 and MRSA infections, with a slow and gradual release. |
| first_indexed | 2024-03-10T18:39:01Z |
| format | Article |
| id | doaj.art-ea31153912224054b5fa8d3c6a0c003d |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T18:39:01Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-ea31153912224054b5fa8d3c6a0c003d2023-11-20T06:02:08ZengMDPI AGAntibiotics2079-63822020-07-019738410.3390/antibiotics9070384Potent and Specific Antibacterial Activity against <i>Escherichia coli</i> O157:H7 and Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) of G17 and G19 Peptides Encapsulated into Poly-Lactic-Co-Glycolic Acid (PLGA) NanoparticlesNicolás Gómez-Sequeda0Jennifer Ruiz1Claudia Ortiz2Mauricio Urquiza3Rodrigo Torres4Grupo de Investigación en Bioquímica y Microbiología (GIBIM), Universidad Industrial de Santander, Bucaramanga 680002, ColombiaGrupo de Investigación en Bioquímica y Microbiología (GIBIM), Universidad Industrial de Santander, Bucaramanga 680002, ColombiaGrupo de Investigación en Bioquímica y Microbiología (GIBIM), Universidad Industrial de Santander, Bucaramanga 680002, ColombiaDepartamento de Química, Universidad Nacional de Colombia, Bogotá 111321, ColombiaDepartamento de Ciencias Básicas, Facultad de Salud, Universidad Industrial de Santander, Bucaramanga 680002, ColombiaAntimicrobial peptides constitute an excellent alternative against conventional antibiotics because of their potent antimicrobial spectrum, unspecific action mechanism and low capacity to produce antibiotic resistance. However, a potential use of these biological molecules as therapeutic agents is threatened by their low stability and susceptibility to proteases. In order to overcome these limitations, encapsulation in biocompatible polymers as poly-lactic-glycolic-acid (PLGA) is a promising alternative for increasing their stability and bioavailability. In this work, the effect of new synthetic antimicrobial peptides GIBIM-P5S9K (G17) and GAM019 (G19) encapsulated on PLGA and acting against methicillin resistant <i>Staphylococus aureus</i> (MRSA) and <i>Escherichia coli</i> O157:H7 was studied. PLGA encapsulation allowed us to load around 7 µg AMPs/mg PLGA with an efficiency of 90.5%, capsule sizes around 290 nm and positive charges. Encapsulation improved antimicrobial activity, decreasing MIC50 from 1.5 to 0.2 (G17NP) and 0.7 (G19NP) µM against MRSA, and from 12.5 to 3.13 µM for <i>E. coli</i> O157:H7. Peptide loaded nanoparticles could be a bacteriostatic drug with potential application to treat these bacterial <i>E. coli</i> O157:H7 and MRSA infections, with a slow and gradual release.https://www.mdpi.com/2079-6382/9/7/384nanoencapsulationantimicrobial peptidesMRSA<i>E. coli</i> O157:H7 |
| spellingShingle | Nicolás Gómez-Sequeda Jennifer Ruiz Claudia Ortiz Mauricio Urquiza Rodrigo Torres Potent and Specific Antibacterial Activity against <i>Escherichia coli</i> O157:H7 and Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) of G17 and G19 Peptides Encapsulated into Poly-Lactic-Co-Glycolic Acid (PLGA) Nanoparticles Antibiotics nanoencapsulation antimicrobial peptides MRSA <i>E. coli</i> O157:H7 |
| title | Potent and Specific Antibacterial Activity against <i>Escherichia coli</i> O157:H7 and Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) of G17 and G19 Peptides Encapsulated into Poly-Lactic-Co-Glycolic Acid (PLGA) Nanoparticles |
| title_full | Potent and Specific Antibacterial Activity against <i>Escherichia coli</i> O157:H7 and Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) of G17 and G19 Peptides Encapsulated into Poly-Lactic-Co-Glycolic Acid (PLGA) Nanoparticles |
| title_fullStr | Potent and Specific Antibacterial Activity against <i>Escherichia coli</i> O157:H7 and Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) of G17 and G19 Peptides Encapsulated into Poly-Lactic-Co-Glycolic Acid (PLGA) Nanoparticles |
| title_full_unstemmed | Potent and Specific Antibacterial Activity against <i>Escherichia coli</i> O157:H7 and Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) of G17 and G19 Peptides Encapsulated into Poly-Lactic-Co-Glycolic Acid (PLGA) Nanoparticles |
| title_short | Potent and Specific Antibacterial Activity against <i>Escherichia coli</i> O157:H7 and Methicillin Resistant <i>Staphylococcus aureus</i> (MRSA) of G17 and G19 Peptides Encapsulated into Poly-Lactic-Co-Glycolic Acid (PLGA) Nanoparticles |
| title_sort | potent and specific antibacterial activity against i escherichia coli i o157 h7 and methicillin resistant i staphylococcus aureus i mrsa of g17 and g19 peptides encapsulated into poly lactic co glycolic acid plga nanoparticles |
| topic | nanoencapsulation antimicrobial peptides MRSA <i>E. coli</i> O157:H7 |
| url | https://www.mdpi.com/2079-6382/9/7/384 |
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