Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics
In this review, the experimental set-up and functional characteristics of single-wavelength and broad-band femtosecond upconversion spectrophotofluorometers developed in our laboratory are described. We discuss applications of this technique to biophysical problems, such as ultrafast fluorescence qu...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/1/211 |
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| author | Simin Cao Haoyang Li Zenan Zhao Sanjun Zhang Jinquan Chen Jianhua Xu Jay R. Knutson Ludwig Brand |
| author_facet | Simin Cao Haoyang Li Zenan Zhao Sanjun Zhang Jinquan Chen Jianhua Xu Jay R. Knutson Ludwig Brand |
| author_sort | Simin Cao |
| collection | DOAJ |
| description | In this review, the experimental set-up and functional characteristics of single-wavelength and broad-band femtosecond upconversion spectrophotofluorometers developed in our laboratory are described. We discuss applications of this technique to biophysical problems, such as ultrafast fluorescence quenching and solvation dynamics of tryptophan, peptides, proteins, reduced nicotinamide adenine dinucleotide (NADH), and nucleic acids. In the tryptophan dynamics field, especially for proteins, two types of solvation dynamics on different time scales have been well explored: ~1 ps for bulk water, and tens of picoseconds for “biological water”, a term that combines effects of water and macromolecule dynamics. In addition, some proteins also show quasi-static self-quenching (QSSQ) phenomena. Interestingly, in our more recent work, we also find that similar mixtures of quenching and solvation dynamics occur for the metabolic cofactor NADH. In this review, we add a brief overview of the emerging development of fluorescent RNA aptamers and their potential application to live cell imaging, while noting how ultrafast measurement may speed their optimization. |
| first_indexed | 2024-03-10T13:31:29Z |
| format | Article |
| id | doaj.art-ea349024c0e940a185b3fcf29a449da0 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T13:31:29Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ea349024c0e940a185b3fcf29a449da02023-11-21T07:58:23ZengMDPI AGMolecules1420-30492021-01-0126121110.3390/molecules26010211Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in BiophysicsSimin Cao0Haoyang Li1Zenan Zhao2Sanjun Zhang3Jinquan Chen4Jianhua Xu5Jay R. Knutson6Ludwig Brand7State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, ChinaState Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, ChinaState Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, ChinaState Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, ChinaState Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, ChinaState Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200062, ChinaLaboratory for Advanced Microscopy and Biophotonics, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USADepartment of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USAIn this review, the experimental set-up and functional characteristics of single-wavelength and broad-band femtosecond upconversion spectrophotofluorometers developed in our laboratory are described. We discuss applications of this technique to biophysical problems, such as ultrafast fluorescence quenching and solvation dynamics of tryptophan, peptides, proteins, reduced nicotinamide adenine dinucleotide (NADH), and nucleic acids. In the tryptophan dynamics field, especially for proteins, two types of solvation dynamics on different time scales have been well explored: ~1 ps for bulk water, and tens of picoseconds for “biological water”, a term that combines effects of water and macromolecule dynamics. In addition, some proteins also show quasi-static self-quenching (QSSQ) phenomena. Interestingly, in our more recent work, we also find that similar mixtures of quenching and solvation dynamics occur for the metabolic cofactor NADH. In this review, we add a brief overview of the emerging development of fluorescent RNA aptamers and their potential application to live cell imaging, while noting how ultrafast measurement may speed their optimization.https://www.mdpi.com/1420-3049/26/1/211tryptophanNADHfluorescent RNA aptamerupconversionquasi-static self-quenchingsolvation dynamics |
| spellingShingle | Simin Cao Haoyang Li Zenan Zhao Sanjun Zhang Jinquan Chen Jianhua Xu Jay R. Knutson Ludwig Brand Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics Molecules tryptophan NADH fluorescent RNA aptamer upconversion quasi-static self-quenching solvation dynamics |
| title | Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics |
| title_full | Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics |
| title_fullStr | Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics |
| title_full_unstemmed | Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics |
| title_short | Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics |
| title_sort | ultrafast fluorescence spectroscopy via upconversion and its applications in biophysics |
| topic | tryptophan NADH fluorescent RNA aptamer upconversion quasi-static self-quenching solvation dynamics |
| url | https://www.mdpi.com/1420-3049/26/1/211 |
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