Computational Design of gRNAs Targeting Genetic Variants Across HIV-1 Subtypes for CRISPR-Mediated Antiviral Therapy

Computational Design of gRNAs Targeting Genetic Variants Across HIV-1 Subtypes for CRISPR-Mediated Antiviral Therapy

Clustered regularly interspaced short palindromic repeats (CRISPR)-based HIV-1 genome editing has shown promising outcomes in in vitro and in vivo viral infection models. However, existing HIV-1 sequence variants have been shown to reduce CRISPR-mediated efficiency and induce viral escape. Two metri...

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Bibliographic Details
Main Authors: Cheng-Han Chung, Alexander G. Allen, Andrew Atkins, Robert W. Link, Michael R. Nonnemacher, Will Dampier, Brian Wigdahl
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
human immunodeficiency virus type 1 (HIV-1)
CRISPR/Cas9
gene therapy
genetic variation
HIV-1 subtypes
bioinformatics
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2021.593077/full

Internet

https://www.frontiersin.org/articles/10.3389/fcimb.2021.593077/full

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