Effects of feeding different levels of acetic acid, probiotic, Oxytetracycline and Neomycin on populations of Coliform and Lactobacillus in pupae of honey bee (ApismelliferaL(
Introduction: Environmental factors and diet are of the most important factors that affect the microbial population of the gastrointestinal tract and there are many antimicrobial systems in honey bees and their foods naturally. The purpose of this study was to investigate the effect of dietary aceti...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Isfahan
2015-09-01
|
| Series: | Biological Journal of Microorganism |
| Subjects: | |
| Online Access: | http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-581-1&slc_lang=en&sid=1 |
| _version_ | 1811327460053090304 |
|---|---|
| author | Abbasali Gheisari Mohammad Bejatian |
| author_facet | Abbasali Gheisari Mohammad Bejatian |
| author_sort | Abbasali Gheisari |
| collection | DOAJ |
| description | Introduction: Environmental factors and diet are of
the most important factors that affect the microbial population of the
gastrointestinal tract and there are many antimicrobial systems in honey bees
and their foods naturally. The purpose of this study was to investigate the
effect of dietary acetic acid and concentrated mixture of probiotic and
antibiotics of oxytetracycline and neomycin on population coliform bacteria and
lactobacilli changes in honey bee pupae. Materials and methods: The experimental treatments consisted
of different levels of acetic acid 5 % (10 , 20 and 30 g/l), protexin
probiotic, Oxytetracycline 20 % and Neomycin 20 % antibiotics with levels of
0.1, 0.2 and 0.3 g/l and sugar syrup 50 % and honey syrup 50 % was performed in
a completely randomized design with four replications for 20 days. After
feeding the experimental groups, one gram of 18 day old worker bees' pupae
samples of each colony was poured into 9 ml of physiological solution. After
preparing different dilutions, each sample was inoculated on MRS agar and
MacConkey agar medium. Lactobacillus and coliform colonies were counted after
finishing incubation period. Results: The results showed that the highest population of coliform bacteria
treated with sugar syrup (2.58 log CFU/g) and the lowest in treatments 0.2 and
0.3 g Neomycin, Oxytetracycline, and probiotic that were equal to zero (P
value <0.05). The highest population of Lactobacillus bacteria belonged
to 0.2 g probiotic treatment (4.05 log CFU/g) and the lowest was for 0.2 and
0.3 g Neomycin treatment which was equal to zero (P value<0.05). Discussion and conclusion: This study showed that the addition probiotic in sugar syrup for
feeding larvae resulted in increasing population of Lactobacilli in the body of
the bee pupae than the control groups. |
| first_indexed | 2024-04-13T15:07:04Z |
| format | Article |
| id | doaj.art-ea3635a3323649da83bc2cfb1b6c212a |
| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-04-13T15:07:04Z |
| publishDate | 2015-09-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-ea3635a3323649da83bc2cfb1b6c212a2022-12-22T02:42:07ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812015-09-0141493100Effects of feeding different levels of acetic acid, probiotic, Oxytetracycline and Neomycin on populations of Coliform and Lactobacillus in pupae of honey bee (ApismelliferaL(Abbasali Gheisari0Mohammad Bejatian1 Associate Professor of Poultry Nutrition, Isfahan Agricultural Research Center, Isfahan, Iran M.Sc. of Animal Science, Isfahan Agricultural Research Center, Isfahan, Iran Introduction: Environmental factors and diet are of the most important factors that affect the microbial population of the gastrointestinal tract and there are many antimicrobial systems in honey bees and their foods naturally. The purpose of this study was to investigate the effect of dietary acetic acid and concentrated mixture of probiotic and antibiotics of oxytetracycline and neomycin on population coliform bacteria and lactobacilli changes in honey bee pupae. Materials and methods: The experimental treatments consisted of different levels of acetic acid 5 % (10 , 20 and 30 g/l), protexin probiotic, Oxytetracycline 20 % and Neomycin 20 % antibiotics with levels of 0.1, 0.2 and 0.3 g/l and sugar syrup 50 % and honey syrup 50 % was performed in a completely randomized design with four replications for 20 days. After feeding the experimental groups, one gram of 18 day old worker bees' pupae samples of each colony was poured into 9 ml of physiological solution. After preparing different dilutions, each sample was inoculated on MRS agar and MacConkey agar medium. Lactobacillus and coliform colonies were counted after finishing incubation period. Results: The results showed that the highest population of coliform bacteria treated with sugar syrup (2.58 log CFU/g) and the lowest in treatments 0.2 and 0.3 g Neomycin, Oxytetracycline, and probiotic that were equal to zero (P value <0.05). The highest population of Lactobacillus bacteria belonged to 0.2 g probiotic treatment (4.05 log CFU/g) and the lowest was for 0.2 and 0.3 g Neomycin treatment which was equal to zero (P value<0.05). Discussion and conclusion: This study showed that the addition probiotic in sugar syrup for feeding larvae resulted in increasing population of Lactobacilli in the body of the bee pupae than the control groups.http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-581-1&slc_lang=en&sid=1Honey bee feeding Pupae Oxytetracycline Probiotic Acetic acid Coliform Lactobacillus |
| spellingShingle | Abbasali Gheisari Mohammad Bejatian Effects of feeding different levels of acetic acid, probiotic, Oxytetracycline and Neomycin on populations of Coliform and Lactobacillus in pupae of honey bee (ApismelliferaL( Biological Journal of Microorganism Honey bee feeding Pupae Oxytetracycline Probiotic Acetic acid Coliform Lactobacillus |
| title | Effects of feeding different levels of acetic acid, probiotic, Oxytetracycline and Neomycin on populations of Coliform and Lactobacillus in pupae of honey bee (ApismelliferaL( |
| title_full | Effects of feeding different levels of acetic acid, probiotic, Oxytetracycline and Neomycin on populations of Coliform and Lactobacillus in pupae of honey bee (ApismelliferaL( |
| title_fullStr | Effects of feeding different levels of acetic acid, probiotic, Oxytetracycline and Neomycin on populations of Coliform and Lactobacillus in pupae of honey bee (ApismelliferaL( |
| title_full_unstemmed | Effects of feeding different levels of acetic acid, probiotic, Oxytetracycline and Neomycin on populations of Coliform and Lactobacillus in pupae of honey bee (ApismelliferaL( |
| title_short | Effects of feeding different levels of acetic acid, probiotic, Oxytetracycline and Neomycin on populations of Coliform and Lactobacillus in pupae of honey bee (ApismelliferaL( |
| title_sort | effects of feeding different levels of acetic acid probiotic oxytetracycline and neomycin on populations of coliform and lactobacillus in pupae of honey bee apismelliferal |
| topic | Honey bee feeding Pupae Oxytetracycline Probiotic Acetic acid Coliform Lactobacillus |
| url | http://uijs.ui.ac.ir/bjm//browse.php?a_code=A-10-581-1&slc_lang=en&sid=1 |
| work_keys_str_mv | AT abbasaligheisari effectsoffeedingdifferentlevelsofaceticacidprobioticoxytetracyclineandneomycinonpopulationsofcoliformandlactobacillusinpupaeofhoneybeeapismelliferal AT mohammadbejatian effectsoffeedingdifferentlevelsofaceticacidprobioticoxytetracyclineandneomycinonpopulationsofcoliformandlactobacillusinpupaeofhoneybeeapismelliferal |