Eco-virological survey of Aedes mosquito larvae in selected dengue outbreak areas in Malaysia
Background & objectivesi: Transovarial transmission of dengue virus in the Aedes vectors is now a well-documented phenomenon reported from many parts of the endemic areas in the world, which played an important role in initiating and maintaining the outbreak in human populations. This study inve...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-12-01
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| Series: | Journal of Vector Borne Diseases |
| Subjects: | |
| Online Access: | http://www.mrcindia.org/journal/issues/514327.pdf |
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| author | A. Rohani A.R. Aidil Azahary M. Malinda M.N. Zurainee H. Rozilawati W.M.A. Wan Najdah H.L. Lee |
| author_facet | A. Rohani A.R. Aidil Azahary M. Malinda M.N. Zurainee H. Rozilawati W.M.A. Wan Najdah H.L. Lee |
| author_sort | A. Rohani |
| collection | DOAJ |
| description | Background & objectivesi: Transovarial transmission of dengue virus in the Aedes vectors is now a well-documented phenomenon reported from many parts of the endemic areas in the world, which played an important role in initiating and maintaining the outbreak in human populations. This study investigated the factors affecting breeding habitats and the relationship with transovarial dengue virus in larvae of Aedes aegypti and Ae. albopictus.
Methods: Larval surveillance was conducted in dengue outbreak areas in Malaysia from 2008 until 2009. Sampling was carried out based on habitat type, water condition (substrate type), canopy coverage, temperature and pH at breeding habitats. RT-PCR was performed to detect presence of transovarial dengue virus in larvae collected in the study areas.
Results: A total of 789 breeding habitats were identified during this study and the majority of these breeding sites
were plastic containers (57.46%). Aedes albopictus dominated most of the water condition surveyed, while Ae. aegypti indicated preference toward habitats with clear water. Aedes aegypti was selective in selecting ovipositional sites compared to Ae. albopictus where shaded areas were shown to be the most preferred. From a total of 363 mosquito larvae pools, 23 (6.3%) pools were positive for dengue virus where 18 of them were from Ae. albopictus and five were from Ae. aegypti mosquito larvae pools.
Interpretation & conclusion: This study indicated the presence of transovarial transmission of dengue virus in immature Ae. aegypti and Ae. albopictus in the field. This study also showed that combination of water conditions, canopy coverage, temperature and pH of breeding habitats were the factors affecting the larval population. The study suggested that larval survey programme could serve as a tool not only to monitor the local dengue vector
distribution but also to provide objective information for taking appropriate action by the community against dengue vectors. |
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| format | Article |
| id | doaj.art-ea36f3570ae94c778ed269e278e8e665 |
| institution | Directory Open Access Journal |
| issn | 0972-9062 0972-9062 |
| language | English |
| last_indexed | 2024-12-20T08:31:26Z |
| publishDate | 2014-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
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| series | Journal of Vector Borne Diseases |
| spelling | doaj.art-ea36f3570ae94c778ed269e278e8e6652022-12-21T19:46:42ZengWolters Kluwer Medknow PublicationsJournal of Vector Borne Diseases0972-90620972-90622014-12-01514327332Eco-virological survey of Aedes mosquito larvae in selected dengue outbreak areas in MalaysiaA. RohaniA.R. Aidil AzaharyM. MalindaM.N. ZuraineeH. RozilawatiW.M.A. Wan NajdahH.L. LeeBackground & objectivesi: Transovarial transmission of dengue virus in the Aedes vectors is now a well-documented phenomenon reported from many parts of the endemic areas in the world, which played an important role in initiating and maintaining the outbreak in human populations. This study investigated the factors affecting breeding habitats and the relationship with transovarial dengue virus in larvae of Aedes aegypti and Ae. albopictus. Methods: Larval surveillance was conducted in dengue outbreak areas in Malaysia from 2008 until 2009. Sampling was carried out based on habitat type, water condition (substrate type), canopy coverage, temperature and pH at breeding habitats. RT-PCR was performed to detect presence of transovarial dengue virus in larvae collected in the study areas. Results: A total of 789 breeding habitats were identified during this study and the majority of these breeding sites were plastic containers (57.46%). Aedes albopictus dominated most of the water condition surveyed, while Ae. aegypti indicated preference toward habitats with clear water. Aedes aegypti was selective in selecting ovipositional sites compared to Ae. albopictus where shaded areas were shown to be the most preferred. From a total of 363 mosquito larvae pools, 23 (6.3%) pools were positive for dengue virus where 18 of them were from Ae. albopictus and five were from Ae. aegypti mosquito larvae pools. Interpretation & conclusion: This study indicated the presence of transovarial transmission of dengue virus in immature Ae. aegypti and Ae. albopictus in the field. This study also showed that combination of water conditions, canopy coverage, temperature and pH of breeding habitats were the factors affecting the larval population. The study suggested that larval survey programme could serve as a tool not only to monitor the local dengue vector distribution but also to provide objective information for taking appropriate action by the community against dengue vectors.http://www.mrcindia.org/journal/issues/514327.pdfAedes aegypti; Aedes albopictus; dengue; larval surveillance; virus infection rate |
| spellingShingle | A. Rohani A.R. Aidil Azahary M. Malinda M.N. Zurainee H. Rozilawati W.M.A. Wan Najdah H.L. Lee Eco-virological survey of Aedes mosquito larvae in selected dengue outbreak areas in Malaysia Journal of Vector Borne Diseases Aedes aegypti; Aedes albopictus; dengue; larval surveillance; virus infection rate |
| title | Eco-virological survey of Aedes mosquito larvae in selected dengue outbreak areas in Malaysia |
| title_full | Eco-virological survey of Aedes mosquito larvae in selected dengue outbreak areas in Malaysia |
| title_fullStr | Eco-virological survey of Aedes mosquito larvae in selected dengue outbreak areas in Malaysia |
| title_full_unstemmed | Eco-virological survey of Aedes mosquito larvae in selected dengue outbreak areas in Malaysia |
| title_short | Eco-virological survey of Aedes mosquito larvae in selected dengue outbreak areas in Malaysia |
| title_sort | eco virological survey of aedes mosquito larvae in selected dengue outbreak areas in malaysia |
| topic | Aedes aegypti; Aedes albopictus; dengue; larval surveillance; virus infection rate |
| url | http://www.mrcindia.org/journal/issues/514327.pdf |
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