Lipid Peroxidation as the Mechanism Underlying Polycyclic Aromatic Hydrocarbons and Sunlight Synergistic Toxicity in Dermal Fibroblasts
Light and atmospheric pollution are both independently implicated in cancer induction and premature aging. Evidence has been growing more recently on the toxic synergy between light and pollutants. Polycyclic aromatic hydrocarbons (PAHs) originate from the incomplete combustion of organic matter. So...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/3/1905 |
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| author | Eloïse Larnac Alicia Montoni Valérie Haydont Laurent Marrot Patrick J. Rochette |
| author_facet | Eloïse Larnac Alicia Montoni Valérie Haydont Laurent Marrot Patrick J. Rochette |
| author_sort | Eloïse Larnac |
| collection | DOAJ |
| description | Light and atmospheric pollution are both independently implicated in cancer induction and premature aging. Evidence has been growing more recently on the toxic synergy between light and pollutants. Polycyclic aromatic hydrocarbons (PAHs) originate from the incomplete combustion of organic matter. Some PAHs, such as the Benzo[a]pyrene (BaP), absorb ultraviolet A (UVA) wavelengths and can act as exogenous chromophores, leading to synergistic toxicity through DNA damage and cytotoxicity concomitant to ROS formation. In this study, we shed light on the mechanism underlying the toxic synergy between PAHs and UVA. Using dermal fibroblasts co-exposed to UVA and BaP, we have demonstrated that the photosensitization reaction causes mortality, which is most likely caused by ROS accumulation. We have shown that these ROS are concentrated in the lipids, which causes an important induction of lipid peroxidation and malondialdehyde, by-products of lipid peroxidation. We have also shown the accumulation of bulky DNA damage, most likely generated by these by-products of lipid peroxidation. To our knowledge, this study represents the first one depicting the molecular effects of photo-pollution on dermal skin. |
| first_indexed | 2024-03-08T03:55:07Z |
| format | Article |
| id | doaj.art-ea375a4f96cf41b59041501626656bca |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T03:55:07Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ea375a4f96cf41b59041501626656bca2024-02-09T15:15:05ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01253190510.3390/ijms25031905Lipid Peroxidation as the Mechanism Underlying Polycyclic Aromatic Hydrocarbons and Sunlight Synergistic Toxicity in Dermal FibroblastsEloïse Larnac0Alicia Montoni1Valérie Haydont2Laurent Marrot3Patrick J. Rochette4Centre de Recherche du CHU de Québec, Université Laval, Axe Médecine Régénératrice, Hôpital du Saint-Sacrement, Québec, QC G1S 4L8, CanadaCentre de Recherche du CHU de Québec, Université Laval, Axe Médecine Régénératrice, Hôpital du Saint-Sacrement, Québec, QC G1S 4L8, CanadaAdvanced Research, L’OREAL Research & Innovation, 93600 Aulnay-Sous-Bois, FranceAdvanced Research, L’OREAL Research & Innovation, 93600 Aulnay-Sous-Bois, FranceCentre de Recherche du CHU de Québec, Université Laval, Axe Médecine Régénératrice, Hôpital du Saint-Sacrement, Québec, QC G1S 4L8, CanadaLight and atmospheric pollution are both independently implicated in cancer induction and premature aging. Evidence has been growing more recently on the toxic synergy between light and pollutants. Polycyclic aromatic hydrocarbons (PAHs) originate from the incomplete combustion of organic matter. Some PAHs, such as the Benzo[a]pyrene (BaP), absorb ultraviolet A (UVA) wavelengths and can act as exogenous chromophores, leading to synergistic toxicity through DNA damage and cytotoxicity concomitant to ROS formation. In this study, we shed light on the mechanism underlying the toxic synergy between PAHs and UVA. Using dermal fibroblasts co-exposed to UVA and BaP, we have demonstrated that the photosensitization reaction causes mortality, which is most likely caused by ROS accumulation. We have shown that these ROS are concentrated in the lipids, which causes an important induction of lipid peroxidation and malondialdehyde, by-products of lipid peroxidation. We have also shown the accumulation of bulky DNA damage, most likely generated by these by-products of lipid peroxidation. To our knowledge, this study represents the first one depicting the molecular effects of photo-pollution on dermal skin.https://www.mdpi.com/1422-0067/25/3/1905ultraviolet A (UVA)Benzo[a]pyrene (BaP)lipid peroxidationreactive oxygen species (ROS)skin dermal fibroblasts |
| spellingShingle | Eloïse Larnac Alicia Montoni Valérie Haydont Laurent Marrot Patrick J. Rochette Lipid Peroxidation as the Mechanism Underlying Polycyclic Aromatic Hydrocarbons and Sunlight Synergistic Toxicity in Dermal Fibroblasts International Journal of Molecular Sciences ultraviolet A (UVA) Benzo[a]pyrene (BaP) lipid peroxidation reactive oxygen species (ROS) skin dermal fibroblasts |
| title | Lipid Peroxidation as the Mechanism Underlying Polycyclic Aromatic Hydrocarbons and Sunlight Synergistic Toxicity in Dermal Fibroblasts |
| title_full | Lipid Peroxidation as the Mechanism Underlying Polycyclic Aromatic Hydrocarbons and Sunlight Synergistic Toxicity in Dermal Fibroblasts |
| title_fullStr | Lipid Peroxidation as the Mechanism Underlying Polycyclic Aromatic Hydrocarbons and Sunlight Synergistic Toxicity in Dermal Fibroblasts |
| title_full_unstemmed | Lipid Peroxidation as the Mechanism Underlying Polycyclic Aromatic Hydrocarbons and Sunlight Synergistic Toxicity in Dermal Fibroblasts |
| title_short | Lipid Peroxidation as the Mechanism Underlying Polycyclic Aromatic Hydrocarbons and Sunlight Synergistic Toxicity in Dermal Fibroblasts |
| title_sort | lipid peroxidation as the mechanism underlying polycyclic aromatic hydrocarbons and sunlight synergistic toxicity in dermal fibroblasts |
| topic | ultraviolet A (UVA) Benzo[a]pyrene (BaP) lipid peroxidation reactive oxygen species (ROS) skin dermal fibroblasts |
| url | https://www.mdpi.com/1422-0067/25/3/1905 |
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