Structural Features of Connective Tissue Formed around Resin Implants Subcutaneously Embedded in Dairy Cows
Foreign body reactions (FBRs) are inadvertently observed in invading or artificially embedded materials, triggering inflammation and subsequent fibrotic processes to occur in situ. Here, we assessed the spatiotemporal formation of connective tissue around implanted materials to establish a technique...
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MDPI AG
2023-11-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/23/3700 |
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| author | Yuka Katayama Osamu Ichii Teppei Nakamura Keita Yanase Masaya Hiraishi Takashi Namba Yuki Otani Teppei Ikeda Erika Tsuji Natsuko Tsuzuki Ken Kobayashi Yasuhiro Kon Takanori Nishimura |
| author_facet | Yuka Katayama Osamu Ichii Teppei Nakamura Keita Yanase Masaya Hiraishi Takashi Namba Yuki Otani Teppei Ikeda Erika Tsuji Natsuko Tsuzuki Ken Kobayashi Yasuhiro Kon Takanori Nishimura |
| author_sort | Yuka Katayama |
| collection | DOAJ |
| description | Foreign body reactions (FBRs) are inadvertently observed in invading or artificially embedded materials, triggering inflammation and subsequent fibrotic processes to occur in situ. Here, we assessed the spatiotemporal formation of connective tissue around implanted materials to establish a technique using connective tissue formed by FBRs as xenografts. An acrylic resin implant, comprising a columnar inner rod and a tubular outer cylinder (OC) with several slits, was embedded in adult dairy cows. Tissues formed in the inner rod and OC groups were histologically analyzed at weeks 2, 4, 8, and 12. Edematous tissues with non-collagenous fibers formed for 2 weeks and showed increased cellularity after 4 weeks. The weight, thickness, amounts of total protein, collagen, DNA, and quantitative scores of α-smooth muscle actin-positive myofibroblasts or elastic fibers notably increased after 8 weeks, with condensed collagen fibers showing orientation. Inflammatory cells were primarily localized in tissues close to the OC, and their numbers increased, with the count of CD204+ cells peaking at 8 weeks and declining at 12 weeks. The count of Ki67+ proliferating cells slightly increased in tissues close to the OC; however, the number and lumen of CD31+ vessels increased. These results may help understand FBR-related tissue remodeling. |
| first_indexed | 2024-03-09T01:56:15Z |
| format | Article |
| id | doaj.art-ea386ac13ca24471919ddf2f046dc4c9 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T01:56:15Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-ea386ac13ca24471919ddf2f046dc4c92023-12-08T15:10:45ZengMDPI AGAnimals2076-26152023-11-011323370010.3390/ani13233700Structural Features of Connective Tissue Formed around Resin Implants Subcutaneously Embedded in Dairy CowsYuka Katayama0Osamu Ichii1Teppei Nakamura2Keita Yanase3Masaya Hiraishi4Takashi Namba5Yuki Otani6Teppei Ikeda7Erika Tsuji8Natsuko Tsuzuki9Ken Kobayashi10Yasuhiro Kon11Takanori Nishimura12Laboratory of Agrobiomedical Science, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanLaboratory of Agrobiomedical Science, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanLaboratory of Agrobiomedical Science, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanLaboratory of Agrobiomedical Science, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanLaboratory of Agrobiomedical Science, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, JapanBRAST Livestock Clinic, BRAST Inc., Sapporo 004-0033, JapanDepartment of Biological Safety Research, Chitose Laboratory, Japan Food Research Laboratories, Chitose 066-0052, JapanDepartment of Biological Safety Research, Chitose Laboratory, Japan Food Research Laboratories, Chitose 066-0052, JapanLaboratory of Cell and Tissue Biology, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanLaboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, JapanLaboratory of Agrobiomedical Science, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, JapanForeign body reactions (FBRs) are inadvertently observed in invading or artificially embedded materials, triggering inflammation and subsequent fibrotic processes to occur in situ. Here, we assessed the spatiotemporal formation of connective tissue around implanted materials to establish a technique using connective tissue formed by FBRs as xenografts. An acrylic resin implant, comprising a columnar inner rod and a tubular outer cylinder (OC) with several slits, was embedded in adult dairy cows. Tissues formed in the inner rod and OC groups were histologically analyzed at weeks 2, 4, 8, and 12. Edematous tissues with non-collagenous fibers formed for 2 weeks and showed increased cellularity after 4 weeks. The weight, thickness, amounts of total protein, collagen, DNA, and quantitative scores of α-smooth muscle actin-positive myofibroblasts or elastic fibers notably increased after 8 weeks, with condensed collagen fibers showing orientation. Inflammatory cells were primarily localized in tissues close to the OC, and their numbers increased, with the count of CD204+ cells peaking at 8 weeks and declining at 12 weeks. The count of Ki67+ proliferating cells slightly increased in tissues close to the OC; however, the number and lumen of CD31+ vessels increased. These results may help understand FBR-related tissue remodeling.https://www.mdpi.com/2076-2615/13/23/3700cowconnective tissueforeign body reactioninflammationfibrosis |
| spellingShingle | Yuka Katayama Osamu Ichii Teppei Nakamura Keita Yanase Masaya Hiraishi Takashi Namba Yuki Otani Teppei Ikeda Erika Tsuji Natsuko Tsuzuki Ken Kobayashi Yasuhiro Kon Takanori Nishimura Structural Features of Connective Tissue Formed around Resin Implants Subcutaneously Embedded in Dairy Cows Animals cow connective tissue foreign body reaction inflammation fibrosis |
| title | Structural Features of Connective Tissue Formed around Resin Implants Subcutaneously Embedded in Dairy Cows |
| title_full | Structural Features of Connective Tissue Formed around Resin Implants Subcutaneously Embedded in Dairy Cows |
| title_fullStr | Structural Features of Connective Tissue Formed around Resin Implants Subcutaneously Embedded in Dairy Cows |
| title_full_unstemmed | Structural Features of Connective Tissue Formed around Resin Implants Subcutaneously Embedded in Dairy Cows |
| title_short | Structural Features of Connective Tissue Formed around Resin Implants Subcutaneously Embedded in Dairy Cows |
| title_sort | structural features of connective tissue formed around resin implants subcutaneously embedded in dairy cows |
| topic | cow connective tissue foreign body reaction inflammation fibrosis |
| url | https://www.mdpi.com/2076-2615/13/23/3700 |
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